Restriction map for pF1KB7736
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsoBI       BspDI       BspEI       BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       ClaI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HaeII       HindIII     KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        MwoI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          336  GTCTAC               GT^[AC][GT]AC
AcyI         1159  GACGCC               G[GA]^CG[CT]C
AflIII        248  ACATGT               A^C[GA][CT]GT
BalI          473  TGGCCA               TGG^CCA
BanI           16  GGCACC               G^G[CT][GA]CC
BbsI         1192  GAAGAC               GAAGAC(2/6)
BbvCI         181  GCTGAGG              CCTCAGC(-5/-2)
BciVI         960  GGATAC               GTATCC(6/5)
BfaI          761  CTAG                 C^TAG
BmyI           15  GGGCAC               G[AGT]GC[ACT]^C
BpmI         1075  CTGGAG               CTGGAG(16/14)
BpuAI        1192  GAAGAC               GAAGAC(2/6)
BsaHI        1159  GACGCC               G[GA]^CG[CT]C
BsaI          136  GGTCTC               GGTCTC(1/5)
BsgI          415  CTGCAC               GTGCAG(16/14)
BsmBI         686  GAGACG               CGTCTC(1/5)
BsmFI         626  GGGAC                GGGAC(10/14)
BsmI         1112  GAATGC               GAATGC(1/-1)
Bsp1286I       15  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      512  TGTACA               T^GTACA
BspHI         578  TCATGA               T^CATGA
BspLU11I      248  ACATGT               A^CATGT
BspMI         875  ACCTGC               ACCTGC(4/8)
BsrBI         679  CCGCTC               CCGCTC(-3/-3)
BsrFI         726  GCCGGT               [GA]^CCGG[CT]
BsrGI         512  TGTACA               T^GTACA
BstYI         968  AGATCC               [GA]^GATC[CT]
Cfr10I        726  GCCGGT               [GA]^CCGG[CT]
CpoI          569  CGGACCG              CG^G[AT]CCG
EaeI          473  TGGCCA               [CT]^GGCC[GA]
FauI          678  CCCGC                CCCGC(4/6)
Hin1I        1159  GACGCC               G[GA]^CG[CT]C
HincII        670  GTTAAC               GT[CT]^[GA]AC
HindII        670  GTTAAC               GT[CT]^[GA]AC
HpaI          670  GTTAAC               GTT^AAC
MaeI          761  CTAG                 C^TAG
MflI          968  AGATCC               [GA]^GATC[CT]
MluNI         473  TGGCCA               TGG^CCA
MscI          473  TGGCCA               TGG^CCA
MspA1I        947  CAGCTG               C[AC]G^C[GT]G
NcoI          534  CCATGG               C^CATGG
NdeI          938  CATATG               CA^TATG
NspI          248  ACATGT               [GA]CATG^[CT]
PflMI         616  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         626  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         947  CAGCTG               CAG^CTG
RcaI          578  TCATGA               T^CATGA
RsrII         569  CGGACCG              CG^G[AT]CCG
ScaI          608  AGTACT               AGT^ACT
SspBI         512  TGTACA               T^GTACA
StuI          554  AGGCCT               AGG^CCT
TspRI         778  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        616  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         968  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AclI          283  AACGTT               AA^CGTT
              919  AACGTT              
AcsI          220  AAATTC               [GA]^AATT[CT]
              856  GAATTT              
AlwI          969  GATCC                GGATC(4/5)
              804  GGATC               
AlwNI         617  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1090  CAGCGACTG           
AocI         1153  CCTGAGG              CC^T[ACGT]AGG
             1168  CCTGAGG             
ApoI          220  AAATTC               [GA]^AATT[CT]
              856  GAATTT              
AspI          571  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              582  GACTGTGTC           
BstXI         737  CCAAAGTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              845  CCAAGTGTGTGG        
Bsu36I       1153  CCTGAGG              CC^T[ACGT]AGG
             1168  CCTGAGG             
DraII         410  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              626  GGGACCT             
DsaI          534  CCATGG               C^C[GA][CT]GG
              566  CCACGG              
Eco81I       1153  CCTGAGG              CC^T[ACGT]AGG
             1168  CCTGAGG             
EcoO109I      410  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              626  GGGACCT             
EcoRV         463  GATATC               GAT^ATC
              637  GATATC              
EcoT14I       534  CCATGG               C^C[AT][AT]GG
             1394  CCAAGG              
HgaI            9  GACGC                GACGC(5/10)
             1159  GACGC               
PflFI         571  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              582  GACTGTGTC           
Psp1406I      283  AACGTT               AA^CGTT
              919  AACGTT              
StyI          534  CCATGG               C^C[AT][AT]GG
             1394  CCAAGG              
TaqI          308  TCGA                 T^CGA
             1216  TCGA                
Tsp45I        539  GTGAC                ^GT[GC]AC
             1375  GTCAC               
Tth111I       571  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              582  GACTGTGTC           
TthHB8I       308  TCGA                 T^CGA
             1216  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BcnI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        Tru9I       TseI        
Tsp509I     
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