Restriction map for pF1KB7719
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MunI        NarI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     Van91I      XbaI        
XhoI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII        848  ACATGT               A^C[GA][CT]GT
Asp700I       270  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          694  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1119  CCCGGG               C^[CT]CG[GA]G
AviII        1169  TGCGCA               TGC^GCA
BalI         1001  TGGCCA               TGG^CCA
BanII         931  GAGCCC               G[GA]GC[CT]^C
BcgI          217  CGACGCCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1274  AGATCT               A^GATCT
BsaWI          50  ACCGGA               [AT]^CCGG[AT]
BsmBI        1014  CGTCTC               CGTCTC(1/5)
BsoBI        1119  CCCGGG               C^[CT]CG[GA]G
BspLU11I      848  ACATGT               A^CATGT
BsrFI         236  GCCGGC               [GA]^CCGG[CT]
BstAPI        987  GCATGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         398  CCATACTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        236  GCCGGC               [GA]^CCGG[CT]
DsaI          839  CCATGG               C^C[GA][CT]GG
EarI          576  CTCTTC               CTCTTC(1/4)
EcoNI        1176  CCTCAATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1169  TGCGCA               TGC^GCA
Ksp632I       576  CTCTTC               CTCTTC(1/4)
MluNI        1001  TGGCCA               TGG^CCA
MscI         1001  TGGCCA               TGG^CCA
NaeI          236  GCCGGC               GCC^GGC
NcoI          839  CCATGG               C^CATGG
NgoMIV        236  GCCGGC               G^CCGGC
NheI         1158  GCTAGC               G^CTAGC
NlaIV         491  GGAGCC               GG[ACGT]^[ACGT]CC
PflFI         694  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1087  CTGCAG               CTGCA^G
SexAI         695  ACCTGGT              A^CC[AT]GGT
SfcI         1087  CTGCAG               C^T[GA][CT]AG
SmaI         1119  CCCGGG               CCC^GGG
TaqI         1256  TCGA                 T^CGA
Tth111I       694  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1256  TCGA                 T^CGA
XcmI          714  CCAAAAGGTCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1119  CCCGGG               C^CCGGG
XmaI         1119  CCCGGG               C^CCGGG
XmnI          270  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         886  CGCG                 CG^CG
             1047  CGCG                
AhdI          844  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1069  GACCGCCTGTC         
AspEI         844  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1069  GACCGCCTGTC         
AspHI        1186  GAGCAC               G[AT]GC[AT]^C
             1252  GTGCTC              
BfaI          655  CTAG                 C^TAG
             1159  CTAG                
BsaI           83  GAGACC               GGTCTC(1/5)
              755  GAGACC              
BsiHKAI      1186  GAGCAC               G[AT]GC[AT]^C
             1252  GTGCTC              
BstUI         886  CGCG                 CG^CG
             1047  CGCG                
EaeI           14  CGGCCA               [CT]^GGCC[GA]
             1001  TGGCCA              
Eam1105I      844  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1069  GACCGCCTGTC         
EcoT14I       206  CCAAGG               C^C[AT][AT]GG
              839  CCATGG              
FauI         1113  CCCGC                CCCGC(4/6)
              309  GCGGG               
MaeI          655  CTAG                 C^TAG
             1159  CTAG                
MslI          103  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              258  CATCGGCATG          
MspA1I       1101  CCGCTG               C[AC]G^C[GT]G
             1203  CCGCTG              
MvnI          886  CGCG                 CG^CG
             1047  CGCG                
NspI          848  ACATGT               [GA]CATG^[CT]
              987  GCATGT              
StuI          230  AGGCCT               AGG^CCT
              341  AGGCCT              
StyI          206  CCAAGG               C^C[AT][AT]GG
              839  CCATGG              
TfiI          631  GATTC                G^A[AT]TC
              749  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        Tsp45I      TspRI       
XhoII       
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