Restriction map for pF1KB7711
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AocI        ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        Eam1105I    EarI        
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HinfI       HpaI        KpnI        Ksp632I     MaeI        
MaeIII      MamI        MfeI        MluI        MroI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        ScaI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AfaI          600  GTAC                 GT^AC
AflIII       1020  ACACGT               A^C[GA][CT]GT
Aor51HI       697  AGCGCT               AGC^GCT
BbeI          207  GGCGCC               GGCGC^C
BcgI         1059  CGACTGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          993  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      993  GCTGAGC              GC^T[ACGT]AGC
BsaI         1120  GAGACC               GGTCTC(1/5)
BsgI          791  GTGCAG               GTGCAG(16/14)
BspMI        1229  ACCTGC               ACCTGC(4/8)
BssHII        296  GCGCGC               G^CGCGC
BstXI         836  CCAAGCGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         483  AGATCC               [GA]^GATC[CT]
CelII         993  GCTGAGC              GC^T[ACGT]AGC
DraIII        187  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      697  AGCGCT               AGC^GCT
EcoNI        1194  CCTCTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI          207  GGCGCC               G^GCGCC
KspI          856  CCGCGG               CCGC^GG
MboII         941  TCTTC                GAAGA(8/7)
MflI          483  AGATCC               [GA]^GATC[CT]
NarI          207  GGCGCC               GG^CGCC
PvuII         745  CAGCTG               CAG^CTG
RsaI          600  GTAC                 GT^AC
SacII         856  CCGCGG               CCGC^GG
SbfI         1230  CCTGCAGG             CCTGCA^GG
SexAI         188  ACCAGGT              A^CC[AT]GGT
SfaNI        1210  GATGC                GCATC(5/9)
SfoI          207  GGCGCC               GGC^GCC
Sse8387I     1230  CCTGCAGG             CCTGCA^GG
XhoII         483  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          121  GAATTC               [GA]^AATT[CT]
              498  GAATTC              
Alw44I        282  GTGCAC               G^TGCAC
              974  GTGCAC              
AlwI          484  GATCC                GGATC(4/5)
              449  GGATC               
ApaLI         282  GTGCAC               G^TGCAC
              974  GTGCAC              
ApoI          121  GAATTC               [GA]^AATT[CT]
              498  GAATTC              
AspHI         282  GTGCAC               G[AT]GC[AT]^C
              974  GTGCAC              
BbrPI         355  CACGTG               CAC^GTG
             1021  CACGTG              
BsaAI         355  CACGTG               [CT]AC^GT[GA]
             1021  CACGTG              
BsiEI         227  CGGCCG               CG[GA][CT]^CG
             1047  CGGCCG              
BsiHKAI       282  GTGCAC               G[AT]GC[AT]^C
              974  GTGCAC              
BsmAI        1120  GAGAC                GTCTC(1/5)
              519  GTCTC               
BsrFI          36  GCCGGC               [GA]^CCGG[CT]
              949  GCCGGC              
Cfr10I         36  GCCGGC               [GA]^CCGG[CT]
              949  GCCGGC              
EagI          227  CGGCCG               C^GGCCG
             1047  CGGCCG              
EclXI         227  CGGCCG               C^GGCCG
             1047  CGGCCG              
Eco52I        227  CGGCCG               C^GGCCG
             1047  CGGCCG              
EcoRI         121  GAATTC               G^AATTC
              498  GAATTC              
EcoT14I       140  CCAAGG               C^C[AT][AT]GG
              848  CCAAGG              
HgaI           79  GACGC                GACGC(5/10)
              946  GACGC               
NaeI           36  GCCGGC               GCC^GGC
              949  GCCGGC              
NgoMIV         36  GCCGGC               G^CCGGC
              949  GCCGGC              
NlaIII        393  CATG                 CATG^
              399  CATG                
PmaCI         355  CACGTG               CAC^GTG
             1021  CACGTG              
PmlI          355  CACGTG               CAC^GTG
             1021  CACGTG              
PpuMI         214  GGGTCCC              [GA]G^G[AT]CC[CT]
              851  AGGTCCC             
SfiI          396  GGCCATGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              405  GGCCAGCAAGGCC       
StyI          140  CCAAGG               C^C[AT][AT]GG
              848  CCAAGG              
Tsp509I       122  AATT                 ^AATT
              499  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeII       MboI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       PstI        Sau3AI      Sau96I      ScrFI       
SfcI        SmaI        TaqI        TseI        TspRI       
TthHB8I     XmaCI       XmaI        
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