Restriction map for pF1KB7711
 (Linear) (Six-base) MAPSORT of: KIBB7711.seq  Check: 5799  from: 1  to: 1263



KIBB7711.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AfeI AGC'GCT

Cuts at:      0     699    1263 
   Size:        699     564
 
AflIII A'CryG_T

Cuts at:      0    1020    1263 
   Size:       1020     243
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     415     892    1263 
   Size:        415     477     371

  Fragments arranged by size: 

                 477     415     371
 
AlwNI CAG_nnn'CTG

Cuts at:      0     114     747     750     831    1175    1263 
   Size:        114     633       3      81     344      88

  Fragments arranged by size: 

                 633     344     114      88      81       3
 
ApaLI G'TGCA_C

Cuts at:      0     282     974    1263 
   Size:        282     692     289

  Fragments arranged by size: 

                 692     289     282
 
ApoI r'AATT_y

Cuts at:      0     121     498    1263 
   Size:        121     377     765

  Fragments arranged by size: 

                 765     377     121
 
AvaI C'yCGr_G

Cuts at:      0      24     159     211     303     548     707    1263 
   Size:         24     135      52      92     245     159     556

  Fragments arranged by size: 

                 556     245     159     135      92      52      24
 
BanI G'GyrC_C

Cuts at:      0     166     192     207    1263 
   Size:        166      26      15    1056

  Fragments arranged by size: 

                1056     166      26      15
 
BanII G_rGCy'C

Cuts at:      0      77     695    1000    1012    1193    1263 
   Size:         77     618     305      12     181      70

  Fragments arranged by size: 

                 618     305     181      77      70      12
 
BbeI G_GCGC'C

Cuts at:      0     211    1263 
   Size:        211    1052
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1048    1082    1263 
   Size:       1048      34     181

  Fragments arranged by size: 

                1048     181      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37     306     403     412     679    1219    1263 
   Size:         37     269      97       9     267     540      44

  Fragments arranged by size: 

                 540     269     267      97      44      37       9
 
BlpI GC'TnA_GC

Cuts at:      0     994    1263 
   Size:        994     269
 
Bme1580I G_kGCm'C

Cuts at:      0     169     286     978    1263 
   Size:        169     117     692     285

  Fragments arranged by size: 

                 692     285     169     117
 
BmgBI CAC'GTC

Cuts at:      0     275     287     333    1029    1263 
   Size:        275      12      46     696     234

  Fragments arranged by size: 

                 696     275     234      46      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     591     939    1248    1263 
   Size:        591     348     309      15

  Fragments arranged by size: 

                 591     348     309      15
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1019    1263 
   Size:       1019     244
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88    1000    1009    1263 
   Size:         88     912       9     254

  Fragments arranged by size: 

                 912     254      88       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1114    1263 
   Size:       1114     149
 
BsaAI yAC'GTr

Cuts at:      0     357    1023    1263 
   Size:        357     666     240

  Fragments arranged by size: 

                 666     357     240
 
BsaHI Gr'CG_yC

Cuts at:      0      80     208     947    1263 
   Size:         80     128     739     316

  Fragments arranged by size: 

                 739     316     128      80
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58      85     778     904    1263 
   Size:         58      27     693     126     359

  Fragments arranged by size: 

                 693     359     126      58      27
 
BseYI C'CCAG_C

Cuts at:      0     663     694     740     785     807     865     969    1204 
   Size:        663      31      46      45      22      58     104     235

Cuts at:   1204    1263 
   Size:         59

  Fragments arranged by size: 

                 663     235     104      59      58      46      45      31
                  22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812    1263 
   Size:        812     451
 
BsiEI CG_ry'CG

Cuts at:      0     230    1050    1263 
   Size:        230     820     213

  Fragments arranged by size: 

                 820     230     213
 
BsiHKAI G_wGCw'C

Cuts at:      0     286     978    1263 
   Size:        286     692     285

  Fragments arranged by size: 

                 692     286     285
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     169     286     695     978    1000    1012    1193 
   Size:         77      92     117     409     283      22      12     181

Cuts at:   1193    1263 
   Size:         70

  Fragments arranged by size: 

                 409     283     181     117      92      77      70      22
                  12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1238    1263 
   Size:       1238      25
 
BsrBI CCG'CTC

Cuts at:      0     609     620    1166    1263 
   Size:        609      11     546      97

  Fragments arranged by size: 

                 609     546      97      11
 
BsrFI r'CCGG_y

Cuts at:      0      36     949    1263 
   Size:         36     913     314

  Fragments arranged by size: 

                 913     314      36
 
BssHII G'CGCG_C

Cuts at:      0     296    1263 
   Size:        296     967
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     114     750    1175    1263 
   Size:        114     636     425      88

  Fragments arranged by size: 

                 636     425     114      88
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     843    1263 
   Size:        843     420
 
BstYI r'GATC_y

Cuts at:      0     483    1263 
   Size:        483     780
 
BtgI C'CryG_G

Cuts at:      0     177     856    1044    1263 
   Size:        177     679     188     219

  Fragments arranged by size: 

                 679     219     188     177
 
BtsI GCAGTG_nn'

Cuts at:      0     115    1176    1263 
   Size:        115    1061      87

  Fragments arranged by size: 

                1061     115      87
 
DraIII CAC_nnn'GTG

Cuts at:      0     192    1263 
   Size:        192    1071
 
EaeI y'GGCC_r

Cuts at:      0     227     395     434     845    1047    1130    1263 
   Size:        227     168      39     411     202      83     133

  Fragments arranged by size: 

                 411     227     202     168     133      83      39
 
EagI C'GGCC_G

Cuts at:      0     227    1047    1263 
   Size:        227     820     216

  Fragments arranged by size: 

                 820     227     216
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19      55     463     496    1263 
   Size:         19      36     408      33     767

  Fragments arranged by size: 

                 767     408      36      33      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88    1000    1009    1263 
   Size:         88     912       9     254

  Fragments arranged by size: 

                 912     254      88       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1198    1263 
   Size:       1198      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144     215     315     852     984    1078    1253    1263 
   Size:        144      71     100     537     132      94     175      10

  Fragments arranged by size: 

                 537     175     144     132     100      94      71      10
            
 
EcoRI G'AATT_C

Cuts at:      0     121     498    1263 
   Size:        121     377     765

  Fragments arranged by size: 

                 765     377     121
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     594    1263 
   Size:        594     669
 
HaeII r_GCGC'y

Cuts at:      0     211     626     701    1263 
   Size:        211     415      75     562

  Fragments arranged by size: 

                 562     415     211      75
 
KasI G'GCGC_C

Cuts at:      0     207    1263 
   Size:        207    1056
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1250    1263 
   Size:       1250      13
 
MscI TGG'CCA

Cuts at:      0     397     847    1132    1263 
   Size:        397     450     285     131

  Fragments arranged by size: 

                 450     397     285     131
 
MslI CAynn'nnrTG

Cuts at:      0     391     397     541    1263 
   Size:        391       6     144     722

  Fragments arranged by size: 

                 722     391     144       6
 
MspA1I CmG'CkG

Cuts at:      0     205     720     747     831     858    1263 
   Size:        205     515      27      84      27     405

  Fragments arranged by size: 

                 515     405     205      84      27      27
 
NaeI GCC'GGC

Cuts at:      0      38     951    1263 
   Size:         38     913     312

  Fragments arranged by size: 

                 913     312      38
 
NarI GG'CG_CC

Cuts at:      0     208    1263 
   Size:        208    1055
 
NgoMIV G'CCGG_C

Cuts at:      0      36     949    1263 
   Size:         36     913     314

  Fragments arranged by size: 

                 913     314      36
 
PasI CC'CwG_GG

Cuts at:      0    1154    1263 
   Size:       1154     109
 
PmlI CAC'GTG

Cuts at:      0     357    1023    1263 
   Size:        357     666     240

  Fragments arranged by size: 

                 666     357     240
 
PpuMI rG'GwC_Cy

Cuts at:      0     215     852    1263 
   Size:        215     637     411

  Fragments arranged by size: 

                 637     411     215
 
PstI C_TGCA'G

Cuts at:      0     110     119     314    1148    1235    1263 
   Size:        110       9     195     834      87      28

  Fragments arranged by size: 

                 834     195     110      87      28       9
 
PvuII CAG'CTG

Cuts at:      0     747    1263 
   Size:        747     516
 
SacII CC_GC'GG

Cuts at:      0     859    1263 
   Size:        859     404
 
SanDI GG'GwC_CC

Cuts at:      0     215    1263 
   Size:        215    1048
 
SbfI CC_TGCA'GG

Cuts at:      0    1235    1263 
   Size:       1235      28
 
SexAI A'CCwGG_T

Cuts at:      0     188    1263 
   Size:        188    1075
 
SfcI C'TryA_G

Cuts at:      0     106     115     310    1144    1231    1263 
   Size:        106       9     195     834      87      32

  Fragments arranged by size: 

                 834     195     106      87      32       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     403     412    1263 
   Size:        403       9     851

  Fragments arranged by size: 

                 851     403       9
 
SfoI GGC'GCC

Cuts at:      0     209    1263 
   Size:        209    1054
 
SmaI CCC'GGG

Cuts at:      0     161     213     305     709    1263 
   Size:        161      52      92     404     554

  Fragments arranged by size: 

                 554     404     161      92      52
 
StyI C'CwwG_G

Cuts at:      0     140     848    1263 
   Size:        140     708     415

  Fragments arranged by size: 

                 708     415     140
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     677     961    1263 
   Size:        677     284     302

  Fragments arranged by size: 

                 677     302     284
 
XmaI C'CCGG_G

Cuts at:      0     159     211     303     707    1263 
   Size:        159      52      92     404     556

  Fragments arranged by size: 

                 556     404     159      92      52
 

 Enzymes that do cut:

     AfeI   AflIII     AlfI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EagI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     PasI     PmlI
    PpuMI     PstI    PvuII    SacII    SanDI     SbfI    SexAI     SfcI
     SfiI     SfoI     SmaI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII
     BmtI   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EarI
  EcoICRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI     SapI     ScaI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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