Restriction map for pF1KB7703
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        ApoI        AscI        AseI        
AsnI        AspI        AvrII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        HgaI        HindIII     
HpaI        KasI        MaeI        MaeIII      MamI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NciI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        TaqI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         394  GACGTC               GACGT^C
Acc65I        798  GGTACC               G^GTACC
AcyI          394  GACGTC               G[GA]^CG[CT]C
AhdI         1096  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1204  GTGCAC               G^TGCAC
AocI          478  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       569  AGCGCT               AGC^GCT
ApaLI        1204  GTGCAC               G^TGCAC
Asp718I       798  GGTACC               G^GTACC
AspEI        1096  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1204  GTGCAC               G[AT]GC[AT]^C
AvaI          367  CCCGAG               C^[CT]CG[GA]G
AviII         836  TGCGCA               TGC^GCA
BalI         1063  TGGCCA               TGG^CCA
BsaHI         394  GACGTC               G[GA]^CG[CT]C
BsaI          904  GGTCTC               GGTCTC(1/5)
BsiHKAI      1204  GTGCAC               G[AT]GC[AT]^C
BsmBI         114  CGTCTC               CGTCTC(1/5)
BsoBI         367  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      992  TGTACA               T^GTACA
BsrBI        1227  CCGCTC               CCGCTC(-3/-3)
BsrGI         992  TGTACA               T^GTACA
BssSI         784  CTCGTG               CACGAG(-5/-1)
BstAPI        601  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         665  AGATCC               [GA]^GATC[CT]
Bsu36I        478  CCTGAGG              CC^T[ACGT]AGG
DraIII        584  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1096  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          433  GAAGAG               CTCTTC(1/4)
Eco47III      569  AGCGCT               AGC^GCT
Eco81I        478  CCTGAGG              CC^T[ACGT]AGG
FspI          836  TGCGCA               TGC^GCA
Hin1I         394  GACGTC               G[GA]^CG[CT]C
HincII        757  GTCAAC               GT[CT]^[GA]AC
HindII        757  GTCAAC               GT[CT]^[GA]AC
KpnI          798  GGTACC               GGTAC^C
Ksp632I       433  GAAGAG               CTCTTC(1/4)
KspI         1216  CCGCGG               CCGC^GG
MaeII         395  ACGT                 A^CGT
MflI          665  AGATCC               [GA]^GATC[CT]
MluNI        1063  TGGCCA               TGG^CCA
MscI         1063  TGGCCA               TGG^CCA
NspI           98  ACATGC               [GA]CATG^[CT]
PvuII         521  CAGCTG               CAG^CTG
SacII        1216  CCGCGG               CCGC^GG
SexAI         585  ACCAGGT              A^CC[AT]GGT
SspBI         992  TGTACA               T^GTACA
StuI          198  AGGCCT               AGG^CCT
TfiI          691  GAATC                G^A[AT]TC
XhoII         665  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BsgI         1056  CTGCAC               GTGCAG(16/14)
              599  GTGCAG              
BsmAI         115  GTCTC                GTCTC(1/5)
              905  GTCTC               
BspMI         818  ACCTGC               ACCTGC(4/8)
             1022  ACCTGC              
BsrDI         840  CATTGC               GCAATG(2/0)
              855  CATTGC              
EaeI           81  TGGCCG               [CT]^GGCC[GA]
             1063  TGGCCA              
EcoNI         320  CCTGGCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              580  CCTCCACCAGG         
HaeII         569  AGCGCT               [GA]GCGC^[CT]
             1115  GGCGCT              
NaeI           83  GCCGGC               GCC^GGC
             1028  GCCGGC              
NgoMIV         83  GCCGGC               G^CCGGC
             1028  GCCGGC              
PflMI         583  CCACCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              747  CCAGCTCCTGG         
PleI          166  GACTC                GAGTC(4/5)
              464  GAGTC               
PpuMI         473  AGGTCCC              [GA]G^G[AT]CC[CT]
              879  GGGACCC             
SmlI          170  CTCAAG               C^T[CT][GA]AG
             1012  CTCAAG              
Van91I        583  CCACCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              747  CCAGCTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        Asp700I     AvaII       BanI        
BanII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TseI        TspRI       XcmI        XmnI        
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