Restriction map for pF1KB7703
 (Linear) (Six-base) MAPSORT of: KIBB7703.seq  Check: 1789  from: 1  to: 1248



KIBB7703.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1031    1248 
   Size:       1031     217
 
AatII G_ACGT'C

Cuts at:      0     398    1248 
   Size:        398     850
 
Acc65I G'GTAC_C

Cuts at:      0     798    1248 
   Size:        798     450
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140    1248 
   Size:        140    1108
 
AfeI AGC'GCT

Cuts at:      0     571    1248 
   Size:        571     677
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1101    1248 
   Size:       1101     147
 
AleI CACnn'nnGTG

Cuts at:      0    1037    1127    1248 
   Size:       1037      90     121

  Fragments arranged by size: 

                1037     121      90
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     808     840    1248 
   Size:        808      32     408

  Fragments arranged by size: 

                 808     408      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     146     706     753    1248 
   Size:        146     560      47     495

  Fragments arranged by size: 

                 560     495     146      47
 
ApaI G_GGCC'C

Cuts at:      0     320     619     631    1248 
   Size:        320     299      12     617

  Fragments arranged by size: 

                 617     320     299      12
 
ApaLI G'TGCA_C

Cuts at:      0    1204    1248 
   Size:       1204      44
 
AvaI C'yCGr_G

Cuts at:      0     367    1248 
   Size:        367     881
 
BanI G'GyrC_C

Cuts at:      0     205     592     607     798    1248 
   Size:        205     387      15     191     450

  Fragments arranged by size: 

                 450     387     205     191      15
 
BanII G_rGCy'C

Cuts at:      0     320     619     631    1248 
   Size:        320     299      12     617

  Fragments arranged by size: 

                 617     320     299      12
 
Bme1580I G_kGCm'C

Cuts at:      0     208     320     612     619     631     700     791     854 
   Size:        208     112     292       7      12      69      91      63

Cuts at:    854    1208    1248 
   Size:        354      40

  Fragments arranged by size: 

                 354     292     208     112      91      69      63      40
                  12       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      46     646    1248 
   Size:         46     600     602

  Fragments arranged by size: 

                 602     600      46
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      51    1248 
   Size:         51    1197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     442     463     482    1098    1248 
   Size:        123     319      21      19     616     150

  Fragments arranged by size: 

                 616     319     150     123      21      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     997    1248 
   Size:        155     842     251

  Fragments arranged by size: 

                 842     251     155
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     910    1248 
   Size:        910     338
 
BsaHI Gr'CG_yC

Cuts at:      0     395    1248 
   Size:        395     853
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     808     838    1171    1201    1248 
   Size:        808      30     333      30      47

  Fragments arranged by size: 

                 808     333      47      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439     460     795     898    1248 
   Size:        439      21     335     103     350

  Fragments arranged by size: 

                 439     350     335     103      21
 
BseYI C'CCAG_C

Cuts at:      0     255     523     746     936    1248 
   Size:        255     268     223     190     312

  Fragments arranged by size: 

                 312     268     255     223     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1041    1248 
   Size:        620     421     207

  Fragments arranged by size: 

                 620     421     207
 
BsiHKAI G_wGCw'C

Cuts at:      0    1208    1248 
   Size:       1208      40
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     120    1248 
   Size:        120    1128
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     320     612     619     631     700     791     854 
   Size:        208     112     292       7      12      69      91      63

Cuts at:    854    1208    1248 
   Size:        354      40

  Fragments arranged by size: 

                 354     292     208     112      91      69      63      40
                  12       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     827    1031    1248 
   Size:        827     204     217

  Fragments arranged by size: 

                 827     217     204
 
BsrBI CCG'CTC

Cuts at:      0    1229    1248 
   Size:       1229      19
 
BsrDI GCAATG_nn'

Cuts at:      0     839     854    1248 
   Size:        839      15     394

  Fragments arranged by size: 

                 839     394      15
 
BsrFI r'CCGG_y

Cuts at:      0      83     901    1028    1248 
   Size:         83     818     127     220

  Fragments arranged by size: 

                 818     220     127      83
 
BsrGI T'GTAC_A

Cuts at:      0     992    1248 
   Size:        992     256
 
BssSI C'ACGA_G

Cuts at:      0     784    1248 
   Size:        784     464
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     607    1248 
   Size:        607     641
 
BstYI r'GATC_y

Cuts at:      0     665    1248 
   Size:        665     583
 
Bsu36I CC'TnA_GG

Cuts at:      0     479    1248 
   Size:        479     769
 
BtgI C'CryG_G

Cuts at:      0     305     400    1216    1248 
   Size:        305      95     816      32

  Fragments arranged by size: 

                 816     305      95      32
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     146    1248 
   Size:        146    1102
 
BtsI GCAGTG_nn'

Cuts at:      0     267     303    1248 
   Size:        267      36     945

  Fragments arranged by size: 

                 945     267      36
 
DraIII CAC_nnn'GTG

Cuts at:      0     589    1248 
   Size:        589     659
 
EaeI y'GGCC_r

Cuts at:      0      81    1063    1248 
   Size:         81     982     185

  Fragments arranged by size: 

                 982     185      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     428    1248 
   Size:        428     820
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     140     442     463     482    1098    1248 
   Size:        123      17     302      21      19     616     150

  Fragments arranged by size: 

                 616     302     150     123      21      19      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     324     584    1248 
   Size:        324     260     664

  Fragments arranged by size: 

                 664     324     260
 
EcoO109I rG'GnC_Cy

Cuts at:      0     316     317     474     615     627     628     703     880 
   Size:        316       1     157     141      12       1      75     177

Cuts at:    880     889    1248 
   Size:          9     359

  Fragments arranged by size: 

                 359     316     177     157     141      75      12       9
                   1       1
 
FspI TGC'GCA

Cuts at:      0     838    1248 
   Size:        838     410
 
FspAI rTGC'GCAy

Cuts at:      0     838    1248 
   Size:        838     410
 
HaeII r_GCGC'y

Cuts at:      0     573    1119    1248 
   Size:        573     546     129

  Fragments arranged by size: 

                 573     546     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1087    1119    1171    1203    1248 
   Size:       1087      32      52      32      45

  Fragments arranged by size: 

                1087      52      45      32      32
 
HincII GTy'rAC

Cuts at:      0     759    1248 
   Size:        759     489
 
KpnI G_GTAC'C

Cuts at:      0     802    1248 
   Size:        802     446
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     833     852    1248 
   Size:        833      19     396

  Fragments arranged by size: 

                 833     396      19
 
MscI TGG'CCA

Cuts at:      0    1065    1248 
   Size:       1065     183
 
MslI CAynn'nnrTG

Cuts at:      0     652     955    1037    1127    1201    1248 
   Size:        652     303      82      90      74      47

  Fragments arranged by size: 

                 652     303      90      82      74      47
 
MspA1I CmG'CkG

Cuts at:      0     275     334     523     560    1218    1248 
   Size:        275      59     189      37     658      30

  Fragments arranged by size: 

                 658     275     189      59      37      30
 
NaeI GCC'GGC

Cuts at:      0      85    1030    1248 
   Size:         85     945     218

  Fragments arranged by size: 

                 945     218      85
 
NgoMIV G'CCGG_C

Cuts at:      0      83    1028    1248 
   Size:         83     945     220

  Fragments arranged by size: 

                 945     220      83
 
NspI r_CATG'y

Cuts at:      0     102    1248 
   Size:        102    1146
 
PasI CC'CwG_GG

Cuts at:      0     612     715     885    1248 
   Size:        612     103     170     363

  Fragments arranged by size: 

                 612     363     170     103
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     589     753    1248 
   Size:        589     164     495

  Fragments arranged by size: 

                 589     495     164
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     808     840    1150    1182    1248 
   Size:        808      32     310      32      66

  Fragments arranged by size: 

                 808     310      66      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     474     880    1248 
   Size:        474     406     368

  Fragments arranged by size: 

                 474     406     368
 
PspOMI G'GGCC_C

Cuts at:      0     316     615     627    1248 
   Size:        316     299      12     621

  Fragments arranged by size: 

                 621     316     299      12
 
PstI C_TGCA'G

Cuts at:      0     511     665     985    1248 
   Size:        511     154     320     263

  Fragments arranged by size: 

                 511     320     263     154
 
PvuII CAG'CTG

Cuts at:      0     523    1248 
   Size:        523     725
 
SacII CC_GC'GG

Cuts at:      0    1219    1248 
   Size:       1219      29
 
SanDI GG'GwC_CC

Cuts at:      0     880    1248 
   Size:        880     368
 
SexAI A'CCwGG_T

Cuts at:      0     585    1248 
   Size:        585     663
 
SfcI C'TryA_G

Cuts at:      0     507     661     981    1248 
   Size:        507     154     320     267

  Fragments arranged by size: 

                 507     320     267     154
 
SmlI C'TyrA_G

Cuts at:      0     170    1012    1248 
   Size:        170     842     236

  Fragments arranged by size: 

                 842     236     170
 
StuI AGG'CCT

Cuts at:      0     200    1248 
   Size:        200    1048
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     370    1248 
   Size:         55     315     878

  Fragments arranged by size: 

                 878     315      55
 
TatI w'GTAC_w

Cuts at:      0     967     992    1248 
   Size:        967      25     256

  Fragments arranged by size: 

                 967     256      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424    1248 
   Size:        392      32     824

  Fragments arranged by size: 

                 824     392      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     520     547     619    1073    1248 
   Size:        520      27      72     454     175

  Fragments arranged by size: 

                 520     454     175      72      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     437    1048    1165    1248 
   Size:        437     611     117      83

  Fragments arranged by size: 

                 611     437     117      83
 
ZraI GAC'GTC

Cuts at:      0     396    1248 
   Size:        396     852
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FspI    FspAI
    HaeII    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NspI     PasI    PflMI     PpiI    PpuMI   PspOMI
     PstI    PvuII    SacII    SanDI    SexAI     SfcI     SmlI     StuI
    TaqII     TatI     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII   AflIII     AgeI     AlfI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI   BssHII    BstBI   BstEII    BstXI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI  HindIII     HpaI     KasI     MfeI     MluI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI
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