Restriction map for pF1KB7663
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwNI       Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstEII      BstPI       BstUI       
BstYI       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MflI        
MluI        MroI        MspA1I      MspI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NspI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          335  AAATTT               [GA]^AATT[CT]
Alw44I        725  GTGCAC               G^TGCAC
AocI           82  CCTCAGG              CC^T[ACGT]AGG
ApaLI         725  GTGCAC               G^TGCAC
ApoI          335  AAATTT               [GA]^AATT[CT]
AseI          144  ATTAAT               AT^TAAT
AsnI          144  ATTAAT               AT^TAAT
Asp700I       808  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         587  GGACC                G^G[AT]CC
BalI          897  TGGCCA               TGG^CCA
BclI          768  TGATCA               T^GATCA
BsaJI         971  CCATGG               C^C[ACGT][ACGT]GG
BsmAI           3  GTCTC                GTCTC(1/5)
BsrDI         637  GCAATG               GCAATG(2/0)
BssKI         386  CCAGG                ^CC[ACGT]GG
Bst1107I      870  GTATAC               GTA^TAC
BstBI         815  TTCGAA               TT^CGAA
BstNI         386  CCAGG                CC^[AT]GG
BstZ17I       870  GTATAC               GTA^TAC
Bsu36I         82  CCTCAGG              CC^T[ACGT]AGG
DraII         570  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          971  CCATGG               C^C[GA][CT]GG
EaeI          897  TGGCCA               [CT]^GGCC[GA]
Eco81I         82  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      570  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        386  CCAGG                ^CC[AT]GG
EcoT14I       971  CCATGG               C^C[AT][AT]GG
EcoT22I       542  ATGCAT               ATGCA^T
FbaI          768  TGATCA               T^GATCA
MfeI          644  CAATTG               C^AATTG
MluNI         897  TGGCCA               TGG^CCA
MscI          897  TGGCCA               TGG^CCA
MslI          702  CACACTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          644  CAATTG               C^AATTG
MvaI          386  CCAGG                CC^[AT]GG
MwoI          634  GCAGCAATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          971  CCATGG               C^CATGG
NsiI          542  ATGCAT               ATGCA^T
NspV          815  TTCGAA               TT^CGAA
PshBI         144  ATTAAT               AT^TAAT
ScrFI         386  CCAGG                CC^[ACGT]GG
SfuI          815  TTCGAA               TT^CGAA
SpeI          279  ACTAGT               A^CTAGT
StuI          691  AGGCCT               AGG^CCT
StyI          971  CCATGG               C^C[AT][AT]GG
XmnI          808  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          131  GTCTAC               GT^[AC][GT]AC
              870  GTATAC              
AlwI          649  GATCC                GGATC(4/5)
              389  GGATC               
AspHI         725  GTGCAC               G[AT]GC[AT]^C
             1000  GAGCAC              
BbvI          468  GCAGC                GCAGC(8/12)
              634  GCAGC               
BsgI           13  GTGCAG               GTGCAG(16/14)
              215  GTGCAG              
BsiHKAI       725  GTGCAC               G[AT]GC[AT]^C
             1000  GAGCAC              
BstXI         701  CCACACTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              779  CCAAGTATCTGG        
Cac8I         411  GCAAGC               GC[ACGT]^[ACGT]GC
              568  GCAGGC              
Cfr13I        571  GGCCC                G^G[ACGT]CC
              587  GGACC               
Fnu4HI        468  GCAGC                GC^[ACGT]GC
              634  GCAGC               
HinfI         604  GATTC                G^A[ACGT]TC
              829  GAATC               
ItaI          468  GCAGC                GC^[ACGT]GC
              634  GCAGC               
PstI          466  CTGCAG               CTGCA^G
              534  CTGCAG              
Sau96I        571  GGCCC                G^G[ACGT]CC
              587  GGACC               
SfcI          466  CTGCAG               C^T[GA][CT]AG
              534  CTGCAG              
TaqI          126  TCGA                 T^CGA
              816  TCGA                
TfiI          604  GATTC                G^A[AT]TC
              829  GAATC               
TseI          468  GCAGC                G^C[AT]GC
              634  GCAGC               
Tsp45I        422  GTCAC                ^GT[GC]AC
              521  GTGAC               
TthHB8I       126  TCGA                 T^CGA
              816  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AluI        BfaI        BmyI        Bsp1286I    BsrI        
BstF5I      DdeI        DpnII       FokI        HaeIII      
HphI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        NdeII       NlaIII      Sau3AI      
SfaNI       Tru9I       Tsp509I     TspRI       
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