Restriction map for pF1KB7631
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AgeI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvrII       BalI        BamHI       BbvCI       
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaBI       BsaI        
BseAI       BseRI       BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SalI        ScaI        SfiI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI           31  GTAGAC               GT^[AC][GT]AC
AflIII        593  ACGCGT               A^C[GA][CT]GT
AhdI          748  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI            5  AGCT                 AG^CT
Alw44I        205  GTGCAC               G^TGCAC
AlwI          444  GATCC                GGATC(4/5)
AlwNI         481  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       398  AGCGCT               AGC^GCT
ApaI          543  GGGCCC               GGGCC^C
ApaLI         205  GTGCAC               G^TGCAC
AspEI         748  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         449  TGCGCA               TGC^GCA
BbsI          130  GAAGAC               GAAGAC(2/6)
BcgI          783  CGAGAGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          745  CTAG                 C^TAG
BpuAI         130  GAAGAC               GAAGAC(2/6)
BsaWI         900  ACCGGA               [AT]^CCGG[AT]
BsgI          402  CTGCAC               GTGCAG(16/14)
BsiEI         324  CGACCG               CG[GA][CT]^CG
BsmAI         388  GAGAC                GTCTC(1/5)
BspMI         116  ACCTGC               ACCTGC(4/8)
BsrI          894  CCAGT                ACTGG(1/-1)
BstBI         127  TTCGAA               TT^CGAA
BstF5I        459  CATCC                GGATG(2/0)
BstXI         758  CCAGCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         443  AGATCC               [GA]^GATC[CT]
DpnII         444  GATC                 ^GATC
DraII         473  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      748  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      398  AGCGCT               AGC^GCT
EcoO109I      473  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       434  CCAAGG               C^C[AT][AT]GG
FokI          459  CATCC                GGATG(9/13)
FspI          449  TGCGCA               TGC^GCA
HphI          898  TCACC                GGTGA(8/7)
MaeI          745  CTAG                 C^TAG
MaeIII        897  GTCAC                ^GT[ACGT]AC
MboI          444  GATC                 ^GATC
MflI          443  AGATCC               [GA]^GATC[CT]
MluI          593  ACGCGT               A^CGCGT
NdeII         444  GATC                 ^GATC
NspI          237  GCATGT               [GA]CATG^[CT]
NspV          127  TTCGAA               TT^CGAA
PstI          478  CTGCAG               CTGCA^G
SapI          178  GAAGAGC              GCTCTTC(1/4)
Sau3AI        444  GATC                 ^GATC
SbfI          477  CCTGCAGG             CCTGCA^GG
SexAI         950  ACCAGGT              A^CC[AT]GGT
SfuI          127  TTCGAA               TT^CGAA
SmlI          394  CTCAAG               C^T[CT][GA]AG
Sse8387I      477  CCTGCAGG             CCTGCA^GG
StuI          380  AGGCCT               AGG^CCT
StyI          434  CCAAGG               C^C[AT][AT]GG
Tsp45I        897  GTCAC                ^GT[GC]AC
TspRI         916  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         443  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII         114  GGACC                G^G[AT]CC
              138  GGACC               
BanII         543  GGGCCC               G[GA]GC[CT]^C
              786  GAGCCC              
BbrPI         154  CACGTG               CAC^GTG
              224  CACGTG              
BsaAI         154  CACGTG               [CT]AC^GT[GA]
              224  CACGTG              
BstAPI        293  GCAAGGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              480  GCAGGCTCTGC         
DdeI          364  CTGAG                C^T[ACGT]AG
              827  CTGAG               
DsaI          197  CCGCGG               C^C[GA][CT]GG
              561  CCGCGG              
EarI          178  GAAGAG               CTCTTC(1/4)
              723  GAAGAG              
Ksp632I       178  GAAGAG               CTCTTC(1/4)
              723  GAAGAG              
KspI          197  CCGCGG               CCGC^GG
              561  CCGCGG              
PmaCI         154  CACGTG               CAC^GTG
              224  CACGTG              
PmlI          154  CACGTG               CAC^GTG
              224  CACGTG              
SacII         197  CCGCGG               CCGC^GG
              561  CCGCGG              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AcyI        AspHI       AvaI        
BanI        BbeI        BbvI        BcnI        BglI        
BmyI        BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        BsoBI       Bsp1286I    BsrBI       BssHII      
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        KasI        
MaeII       MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NlaIII      NlaIV       PleI        Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        TaqI        
TseI        TthHB8I     XmaCI       XmaI        
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