Restriction map for pF1KB7621
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BseRI       BsgI        BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BssSI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MamI        
MboI        MfeI        MflI        MluI        MluNI       
MscI        MslI        MunI        NdeI        NdeII       
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        638  TCCGGA               T^CCGGA
ApaI          436  GGGCCC               GGGCC^C
AvaI          180  CCCGGG               C^[CT]CG[GA]G
BbrPI         130  CACGTG               CAC^GTG
BbsI          855  GTCTTC               GAAGAC(2/6)
BbvCI         808  CCTCAGC              CCTCAGC(-5/-2)
BpuAI         855  GTCTTC               GAAGAC(2/6)
BseAI         638  TCCGGA               T^CCGGA
BsmAI         468  GTCTC                GTCTC(1/5)
BsoBI         180  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      575  TGTACA               T^GTACA
BspEI         638  TCCGGA               T^CCGGA
BspMI         684  GCAGGT               ACCTGC(4/8)
BsrGI         575  TGTACA               T^GTACA
BssHII        170  GCGCGC               G^CGCGC
BstEII        124  GGTTACC              G^GT[ACGT]ACC
BstPI         124  GGTTACC              G^GT[ACGT]ACC
BstXI         266  CCAACGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         816  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      816  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       124  GGTTACC              G^GT[ACGT]ACC
EcoT14I       311  CCATGG               C^C[AT][AT]GG
HinfI         846  GAGTC                G^A[ACGT]TC
KspI          254  CCGCGG               CCGC^GG
MroI          638  TCCGGA               T^CCGGA
MseI          689  TTAA                 T^TAA
NcoI          311  CCATGG               C^CATGG
NheI          654  GCTAGC               G^CTAGC
NspI           35  GCATGT               [GA]CATG^[CT]
PflMI         588  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          846  GAGTC                GAGTC(4/5)
PmaCI         130  CACGTG               CAC^GTG
PmlI          130  CACGTG               CAC^GTG
PpuMI         816  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          146  CTGCAG               CTGCA^G
SacII         254  CCGCGG               CCGC^GG
SfiI          185  GGCCATCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          180  CCCGGG               CCC^GGG
SmlI          843  CTTGAG               C^T[CT][GA]AG
SspBI         575  TGTACA               T^GTACA
StyI          311  CCATGG               C^C[AT][AT]GG
TaqI          567  TCGA                 T^CGA
Tru9I         689  TTAA                 T^TAA
Tsp45I        931  GTCAC                ^GT[GC]AC
TspRI         872  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       567  TCGA                 T^CGA
Van91I        588  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         180  CCCGGG               C^CCGGG
XmaI          180  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         589  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              872  CAGTGTCTG           
AvaII         582  GGACC                G^G[AT]CC
              817  GGTCC               
BfaI           44  CTAG                 C^TAG
              655  CTAG                
BglI           56  GCCACTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              186  GCCATCCTGGC         
BpmI          595  CTGGAG               CTGGAG(16/14)
              601  CTGGAG              
BsaAI           4  TACGTG               [CT]AC^GT[GA]
              130  CACGTG              
BsaWI         638  TCCGGA               [AT]^CCGG[AT]
              849  TCCGGT              
BsrFI         195  GCCGGC               [GA]^CCGG[CT]
              513  GCCGGC              
BsrI          227  ACTGG                ACTGG(1/-1)
              907  ACTGG               
Cfr10I        195  GCCGGC               [GA]^CCGG[CT]
              513  GCCGGC              
EcoNI          18  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              808  CCTCAGCCAGG         
MaeI           44  CTAG                 C^TAG
              655  CTAG                
MaeIII        125  GTTAC                ^GT[ACGT]AC
              931  GTCAC               
MboII         738  GAAGA                GAAGA(8/7)
              856  TCTTC               
NaeI          195  GCCGGC               GCC^GGC
              513  GCCGGC              
NgoMIV        195  GCCGGC               G^CCGGC
              513  GCCGGC              
NlaIII         36  CATG                 CATG^
              312  CATG                
NotI          136  GCGGCCGC             GC^GGCCGC
              256  GCGGCCGC            
PvuII         143  CAGCTG               CAG^CTG
              463  CAGCTG              
SfaNI         504  GATGC                GCATC(5/9)
              357  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
BanI        BanII       BbeI        BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiEI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DsaI        EaeI        EagI        EclXI       
Eco52I      EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        KasI        
MaeII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NlaIV       
RsaI        Sau96I      ScrFI       SfcI        SfoI        
TseI        
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