Restriction map for pF1KB7621
 (Linear) (Six-base) MAPSORT of: KIBB7621.seq  Check: 8038  from: 1  to: 939



KIBB7621.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     594     877     939 
   Size:        594     283      62

  Fragments arranged by size: 

                 594     283      62
 
ApaI G_GGCC'C

Cuts at:      0     440     939 
   Size:        440     499
 
AvaI C'yCGr_G

Cuts at:      0     180     939 
   Size:        180     759
 
BanI G'GyrC_C

Cuts at:      0      78     208     250     939 
   Size:         78     130      42     689

  Fragments arranged by size: 

                 689     130      78      42
 
BanII G_rGCy'C

Cuts at:      0     440     674     842     939 
   Size:        440     234     168      97

  Fragments arranged by size: 

                 440     234     168      97
 
BbeI G_GCGC'C

Cuts at:      0      82     212     254     939 
   Size:         82     130      42     685

  Fragments arranged by size: 

                 685     130      82      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     848     939 
   Size:        848      91
 
BbvCI CC'TCA_GC

Cuts at:      0     809     939 
   Size:        809     130
 
BglI GCCn_nnn'nGGC

Cuts at:      0      62     192     939 
   Size:         62     130     747

  Fragments arranged by size: 

                 747     130      62
 
Bme1580I G_kGCm'C

Cuts at:      0     440     939 
   Size:        440     499
 
BmgBI CAC'GTC

Cuts at:      0      30     939 
   Size:         30     909
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     917     939 
   Size:        917      22
 
BmtI G_CTAG'C

Cuts at:      0     658     939 
   Size:        658     281
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     622     939 
   Size:        616       6     317

  Fragments arranged by size: 

                 616     317       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     809     939 
   Size:        809     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     864     939 
   Size:        864      75
 
BsaAI yAC'GTr

Cuts at:      0       6     132     939 
   Size:          6     126     807

  Fragments arranged by size: 

                 807     126       6
 
BsaHI Gr'CG_yC

Cuts at:      0      79     209     251     939 
   Size:         79     130      42     688

  Fragments arranged by size: 

                 688     130      79      42
 
BsaWI w'CCGG_w

Cuts at:      0     638     849     939 
   Size:        638     211      90

  Fragments arranged by size: 

                 638     211      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61     280     310     595     625     939 
   Size:         31      30     219      30     285      30     314

  Fragments arranged by size: 

                 314     285     219      31      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0     666     803     939 
   Size:        666     137     136

  Fragments arranged by size: 

                 666     137     136
 
BsiEI CG_ry'CG

Cuts at:      0     140     260     305     380     939 
   Size:        140     120      45      75     559

  Fragments arranged by size: 

                 559     140     120      75      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     440     674     842     939 
   Size:        440     234     168      97

  Fragments arranged by size: 

                 440     234     168      97
 
BspEI T'CCGG_A

Cuts at:      0     638     939 
   Size:        638     301
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     675     939 
   Size:        675     264
 
BsrFI r'CCGG_y

Cuts at:      0     195     513     939 
   Size:        195     318     426

  Fragments arranged by size: 

                 426     318     195
 
BsrGI T'GTAC_A

Cuts at:      0     575     939 
   Size:        575     364
 
BssHII G'CGCG_C

Cuts at:      0     170     939 
   Size:        170     769
 
BstEII G'GTnAC_C

Cuts at:      0     124     939 
   Size:        124     815
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273     939 
   Size:        273     666
 
BtgI C'CryG_G

Cuts at:      0     254     272     311     939 
   Size:        254      18      39     628

  Fragments arranged by size: 

                 628     254      39      18
 
EaeI y'GGCC_r

Cuts at:      0     137     193     257     302     377     477     939 
   Size:        137      56      64      45      75     100     462

  Fragments arranged by size: 

                 462     137     100      75      64      56      45
 
EagI C'GGCC_G

Cuts at:      0     137     257     302     377     939 
   Size:        137     120      45      75     562

  Fragments arranged by size: 

                 562     137     120      75      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     501     773     939 
   Size:        501     272     166

  Fragments arranged by size: 

                 501     272     166
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     622     939 
   Size:        616       6     317

  Fragments arranged by size: 

                 616     317       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      22     812     939 
   Size:         22     790     127

  Fragments arranged by size: 

                 790     127      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     817     939 
   Size:        817     122
 
HaeII r_GCGC'y

Cuts at:      0      82     212     254     939 
   Size:         82     130      42     685

  Fragments arranged by size: 

                 685     130      82      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     451     483     939 
   Size:        451      32     456

  Fragments arranged by size: 

                 456     451      32
 
KasI G'GCGC_C

Cuts at:      0      78     208     250     939 
   Size:         78     130      42     689

  Fragments arranged by size: 

                 689     130      78      42
 
MspA1I CmG'CkG

Cuts at:      0     145     151     256     451     465     483     591     939 
   Size:        145       6     105     195      14      18     108     348

  Fragments arranged by size: 

                 348     195     145     108     105      18      14       6
            
 
NaeI GCC'GGC

Cuts at:      0     197     515     939 
   Size:        197     318     424

  Fragments arranged by size: 

                 424     318     197
 
NarI GG'CG_CC

Cuts at:      0      79     209     251     939 
   Size:         79     130      42     688

  Fragments arranged by size: 

                 688     130      79      42
 
NcoI C'CATG_G

Cuts at:      0     311     939 
   Size:        311     628
 
NgoMIV G'CCGG_C

Cuts at:      0     195     513     939 
   Size:        195     318     426

  Fragments arranged by size: 

                 426     318     195
 
NheI G'CTAG_C

Cuts at:      0     654     939 
   Size:        654     285
 
NotI GC'GGCC_GC

Cuts at:      0     137     257     939 
   Size:        137     120     682

  Fragments arranged by size: 

                 682     137     120
 
NspI r_CATG'y

Cuts at:      0      39     939 
   Size:         39     900
 
PasI CC'CwG_GG

Cuts at:      0     889     939 
   Size:        889      50
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     594     939 
   Size:        594     345
 
PfoI T'CCnGG_A

Cuts at:      0      17     218     939 
   Size:         17     201     721

  Fragments arranged by size: 

                 721     201      17
 
PmlI CAC'GTG

Cuts at:      0     132     939 
   Size:        132     807
 
PpuMI rG'GwC_Cy

Cuts at:      0     817     939 
   Size:        817     122
 
PspOMI G'GGCC_C

Cuts at:      0     436     939 
   Size:        436     503
 
PstI C_TGCA'G

Cuts at:      0     150     939 
   Size:        150     789
 
PvuII CAG'CTG

Cuts at:      0     145     465     939 
   Size:        145     320     474

  Fragments arranged by size: 

                 474     320     145
 
SacII CC_GC'GG

Cuts at:      0     257     939 
   Size:        257     682
 
SfcI C'TryA_G

Cuts at:      0     146     318     618     939 
   Size:        146     172     300     321

  Fragments arranged by size: 

                 321     300     172     146
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     192     939 
   Size:        192     747
 
SfoI GGC'GCC

Cuts at:      0      80     210     252     939 
   Size:         80     130      42     687

  Fragments arranged by size: 

                 687     130      80      42
 
SmaI CCC'GGG

Cuts at:      0     182     939 
   Size:        182     757
 
SmlI C'TyrA_G

Cuts at:      0     843     939 
   Size:        843      96
 
StyI C'CwwG_G

Cuts at:      0     311     939 
   Size:        311     628
 
TatI w'GTAC_w

Cuts at:      0     575     939 
   Size:        575     364
 
XmaI C'CCGG_G

Cuts at:      0     180     939 
   Size:        180     759
 

 Enzymes that do cut:

    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI Bsp1286I    BspEI
    BspMI    BsrFI    BsrGI   BssHII   BstEII    BstXI     BtgI     EaeI
     EagI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
    SacII     SfcI     SfiI     SfoI     SmaI     SmlI     StyI     TatI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BplI     BsaI    BsaBI    BseRI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI
    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NdeI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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