Restriction map for pF1KB7620
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AhdI          843  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         989  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         843  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI          78  GAGCAC               G[AT]GC[AT]^C
AvaI          439  CCCGGG               C^[CT]CG[GA]G
BbsI         1031  GTCTTC               GAAGAC(2/6)
BfaI          740  CTAG                 C^TAG
BlpI          468  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      468  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1031  GTCTTC               GAAGAC(2/6)
BsaWI         507  TCCGGT               [AT]^CCGG[AT]
BsiHKAI        78  GAGCAC               G[AT]GC[AT]^C
BsoBI         439  CCCGGG               C^[CT]CG[GA]G
BsrBI         111  CCGCTC               CCGCTC(-3/-3)
CelII         468  GCTCAGC              GC^T[ACGT]AGC
EaeI         1071  TGGCCG               [CT]^GGCC[GA]
Eam1105I      843  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MaeI          740  CTAG                 C^TAG
NspI           14  ACATGC               [GA]CATG^[CT]
PstI          868  CTGCAG               CTGCA^G
ScaI          699  AGTACT               AGT^ACT
SmaI          439  CCCGGG               CCC^GGG
SmlI          160  CTCAAG               C^T[CT][GA]AG
StuI          815  AGGCCT               AGG^CCT
TaqI         1035  TCGA                 T^CGA
TthHB8I      1035  TCGA                 T^CGA
XmaCI         439  CCCGGG               C^CCGGG
XmaI          439  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          591  GTAC                 GT^AC
              700  GTAC                
ApaI          667  GGGCCC               GGGCC^C
              733  GGGCCC              
BglI          247  GCCCAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              726  GCCCCAAGGGC         
BsaI           61  GAGACC               GGTCTC(1/5)
               68  GAGACC              
BstXI         774  CCAGCCCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1014  CCAGCCTGTTGG        
BstYI         688  GGATCT               [GA]^GATC[CT]
              959  GGATCT              
DsaI          141  CCATGG               C^C[GA][CT]GG
              408  CCATGG              
FauI          128  CCCGC                CCCGC(4/6)
              749  CCCGC               
HaeII         173  AGCGCC               [GA]GCGC^[CT]
              227  AGCGCC              
MflI          688  GGATCT               [GA]^GATC[CT]
              959  GGATCT              
MslI          205  CACAAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              973  CACCCCTGTG          
NcoI          141  CCATGG               C^CATGG
              408  CCATGG              
PleI           43  GACTC                GAGTC(4/5)
              504  GACTC               
PpuMI         362  AGGTCCC              [GA]G^G[AT]CC[CT]
              527  GGGTCCC             
RsaI          591  GTAC                 GT^AC
              700  GTAC                
SfcI          805  CTACAG               C^T[GA][CT]AG
              868  CTGCAG              
TfiI          907  GATTC                G^A[AT]TC
             1010  GATTC               
XhoII         688  GGATCT               [GA]^GATC[CT]
              959  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BssSI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PvuII       Sau3AI      Sau96I      
ScrFI       StyI        TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com