Restriction map for pF1KB7620
 (Linear) (Six-base) MAPSORT of: KIBB7620.seq  Check: 8815  from: 1  to: 1101



KIBB7620.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     688    1101 
   Size:        688     413
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     784     905    1101 
   Size:        321     463     121     196

  Fragments arranged by size: 

                 463     321     196     121
 
AhdI GACnn_n'nnGTC

Cuts at:      0     848    1101 
   Size:        848     253
 
AleI CACnn'nnGTG

Cuts at:      0     209     977    1101 
   Size:        209     768     124

  Fragments arranged by size: 

                 768     209     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     994    1101 
   Size:        994     107
 
ApaI G_GGCC'C

Cuts at:      0     671     737    1101 
   Size:        671      66     364

  Fragments arranged by size: 

                 671     364      66
 
AvaI C'yCGr_G

Cuts at:      0     439    1101 
   Size:        439     662
 
BanI G'GyrC_C

Cuts at:      0     426     510     640     784    1101 
   Size:        426      84     130     144     317

  Fragments arranged by size: 

                 426     317     144     130      84
 
BanII G_rGCy'C

Cuts at:      0     671     728     737    1101 
   Size:        671      57       9     364

  Fragments arranged by size: 

                 671     364      57       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1024    1101 
   Size:       1024      77
 
BglI GCCn_nnn'nGGC

Cuts at:      0     253     732    1101 
   Size:        253     479     369

  Fragments arranged by size: 

                 479     369     253
 
BlpI GC'TnA_GC

Cuts at:      0     469    1101 
   Size:        469     632
 
Bme1580I G_kGCm'C

Cuts at:      0     671     737     944    1101 
   Size:        671      66     207     157

  Fragments arranged by size: 

                 671     207     157      66
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       2     691    1101 
   Size:          2     689     410

  Fragments arranged by size: 

                 689     410       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     533     634     740     757     842    1019    1101 
   Size:        244     289     101     106      17      85     177      82

  Fragments arranged by size: 

                 289     244     177     106     101      85      82      17
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1096    1101 
   Size:       1096       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145    1101 
   Size:        145     956
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55      62    1101 
   Size:         55       7    1039

  Fragments arranged by size: 

                1039      55       7
 
BsaWI w'CCGG_w

Cuts at:      0     507    1101 
   Size:        507     594
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     315     701     911    1101 
   Size:        315     386     210     190

  Fragments arranged by size: 

                 386     315     210     190
 
BseYI C'CCAG_C

Cuts at:      0     392    1039    1044    1101 
   Size:        392     647       5      57

  Fragments arranged by size: 

                 647     392      57       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      82    1101 
   Size:         82    1019
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     671     728     737     944    1101 
   Size:         82     589      57       9     207     157

  Fragments arranged by size: 

                 589     207     157      82      57       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     130     609     688    1101 
   Size:        130     479      79     413

  Fragments arranged by size: 

                 479     413     130      79
 
BsrBI CCG'CTC

Cuts at:      0     113    1101 
   Size:        113     988
 
BssSI C'ACGA_G

Cuts at:      0     828     892     923    1101 
   Size:        828      64      31     178

  Fragments arranged by size: 

                 828     178      64      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     781    1021    1101 
   Size:        781     240      80

  Fragments arranged by size: 

                 781     240      80
 
BstYI r'GATC_y

Cuts at:      0     688     959    1101 
   Size:        688     271     142

  Fragments arranged by size: 

                 688     271     142
 
BtgI C'CryG_G

Cuts at:      0     141     408    1101 
   Size:        141     267     693

  Fragments arranged by size: 

                 693     267     141
 
BtsI GCAGTG_nn'

Cuts at:      0      30    1101 
   Size:         30    1071
 
EaeI y'GGCC_r

Cuts at:      0    1071    1101 
   Size:       1071      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     190     803     999    1101 
   Size:        190     613     196     102

  Fragments arranged by size: 

                 613     196     190     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     321     533     634     740     757     784     842 
   Size:        244      77     212     101     106      17      27      58

Cuts at:    842     905    1019    1101 
   Size:         63     114      82

  Fragments arranged by size: 

                 244     212     114     106     101      82      77      63
                  58      27      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     187     261     998    1101 
   Size:        187      74     737     103

  Fragments arranged by size: 

                 737     187     103      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0     363     528     668     733     734    1101 
   Size:        363     165     140      65       1     367

  Fragments arranged by size: 

                 367     363     165     140      65       1
 
HaeII r_GCGC'y

Cuts at:      0     177     231    1101 
   Size:        177      54     870

  Fragments arranged by size: 

                 870     177      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     696     728     834     866    1101 
   Size:        696      32     106      32     235

  Fragments arranged by size: 

                 696     235     106      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     142    1101 
   Size:        142     959
 
MslI CAynn'nnrTG

Cuts at:      0     209     977    1101 
   Size:        209     768     124

  Fragments arranged by size: 

                 768     209     124
 
MspA1I CmG'CkG

Cuts at:      0     131     654     867     883    1101 
   Size:        131     523     213      16     218

  Fragments arranged by size: 

                 523     218     213     131      16
 
NcoI C'CATG_G

Cuts at:      0     141     408    1101 
   Size:        141     267     693

  Fragments arranged by size: 

                 693     267     141
 
NspI r_CATG'y

Cuts at:      0      18    1101 
   Size:         18    1083
 
PasI CC'CwG_GG

Cuts at:      0      53    1101 
   Size:         53    1048
 
PfoI T'CCnGG_A

Cuts at:      0     221     499    1101 
   Size:        221     278     602

  Fragments arranged by size: 

                 602     278     221
 
PpuMI rG'GwC_Cy

Cuts at:      0     363     528    1101 
   Size:        363     165     573

  Fragments arranged by size: 

                 573     363     165
 
PspOMI G'GGCC_C

Cuts at:      0     667     733    1101 
   Size:        667      66     368

  Fragments arranged by size: 

                 667     368      66
 
PstI C_TGCA'G

Cuts at:      0     872    1101 
   Size:        872     229
 
PvuII CAG'CTG

Cuts at:      0     654     867     883    1101 
   Size:        654     213      16     218

  Fragments arranged by size: 

                 654     218     213      16
 
SanDI GG'GwC_CC

Cuts at:      0     528    1101 
   Size:        528     573
 
ScaI AGT'ACT

Cuts at:      0     701    1101 
   Size:        701     400
 
SfcI C'TryA_G

Cuts at:      0     805     868    1101 
   Size:        805      63     233

  Fragments arranged by size: 

                 805     233      63
 
SmaI CCC'GGG

Cuts at:      0     441    1101 
   Size:        441     660
 
SmlI C'TyrA_G

Cuts at:      0     160    1101 
   Size:        160     941
 
StuI AGG'CCT

Cuts at:      0     817    1101 
   Size:        817     284
 
StyI C'CwwG_G

Cuts at:      0     141     408     523     729    1101 
   Size:        141     267     115     206     372

  Fragments arranged by size: 

                 372     267     206     141     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     710    1101 
   Size:        710     391
 
TatI w'GTAC_w

Cuts at:      0     699    1101 
   Size:        699     402
 
XmaI C'CCGG_G

Cuts at:      0     439    1101 
   Size:        439     662
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AleI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BssSI    BstXI    BstYI     BtgI     BtsI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MslI   MspA1I
     NcoI     NspI     PasI     PfoI    PpuMI   PspOMI     PstI    PvuII
    SanDI     ScaI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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