Restriction map for pF1KB7611
 (Linear) (Six-base) MAPSORT of: KIBB7611.seq  Check: 7862  from: 1  to: 906



KIBB7611.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     813     906 
   Size:        813      93
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61     634     651     906 
   Size:         61     573      17     255

  Fragments arranged by size: 

                 573     255      61      17
 
ApaI G_GGCC'C

Cuts at:      0     642     664     906 
   Size:        642      22     242

  Fragments arranged by size: 

                 642     242      22
 
ApaLI G'TGCA_C

Cuts at:      0     844     906 
   Size:        844      62
 
AscI GG'CGCG_CC

Cuts at:      0     339     906 
   Size:        339     567
 
AvaI C'yCGr_G

Cuts at:      0     409     615     667     783     906 
   Size:        409     206      52     116     123

  Fragments arranged by size: 

                 409     206     123     116      52
 
BanI G'GyrC_C

Cuts at:      0     402     420     594     619     906 
   Size:        402      18     174      25     287

  Fragments arranged by size: 

                 402     287     174      25      18
 
BanII G_rGCy'C

Cuts at:      0      40     642     664     906 
   Size:         40     602      22     242

  Fragments arranged by size: 

                 602     242      40      22
 
BbeI G_GCGC'C

Cuts at:      0     406     906 
   Size:        406     500
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     552     906 
   Size:        552     354
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     265     299     906 
   Size:        265      34     607

  Fragments arranged by size: 

                 607     265      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     739     906 
   Size:        739     167
 
BlpI GC'TnA_GC

Cuts at:      0      16     906 
   Size:         16     890
 
Bme1580I G_kGCm'C

Cuts at:      0     599     622     642     664     848     906 
   Size:        599      23      20      22     184      58

  Fragments arranged by size: 

                 599     184      58      23      22      20
 
BsaHI Gr'CG_yC

Cuts at:      0     403     906 
   Size:        403     503
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     292     448     906 
   Size:        292     156     458

  Fragments arranged by size: 

                 458     292     156
 
BseYI C'CCAG_C

Cuts at:      0      77     571     906 
   Size:         77     494     335

  Fragments arranged by size: 

                 494     335      77
 
BsiEI CG_ry'CG

Cuts at:      0     683     725     906 
   Size:        683      42     181

  Fragments arranged by size: 

                 683     181      42
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     113     271     848     906 
   Size:         92      21     158     577      58

  Fragments arranged by size: 

                 577     158      92      58      21
 
BsiWI C'GTAC_G

Cuts at:      0     455     906 
   Size:        455     451
 
Bsp1286I G_dGCh'C

Cuts at:      0      40      92     113     271     599     622     642     664 
   Size:         40      52      21     158     328      23      20      22

Cuts at:    664     848     906 
   Size:        184      58

  Fragments arranged by size: 

                 328     184     158      58      52      40      23      22
                  21      20
 
BsrBI CCG'CTC

Cuts at:      0     408     492     906 
   Size:        408      84     414

  Fragments arranged by size: 

                 414     408      84
 
BsrFI r'CCGG_y

Cuts at:      0     335     906 
   Size:        335     571
 
BssHII G'CGCG_C

Cuts at:      0     339     906 
   Size:        339     567
 
BtgI C'CryG_G

Cuts at:      0     590     605     737     890     906 
   Size:        590      15     132     153      16

  Fragments arranged by size: 

                 590     153     132      16      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     564     906 
   Size:        564     342
 
EaeI y'GGCC_r

Cuts at:      0     466     680     722     791     906 
   Size:        466     214      42      69     115

  Fragments arranged by size: 

                 466     214     115      69      42
 
EagI C'GGCC_G

Cuts at:      0     680     722     906 
   Size:        680      42     184

  Fragments arranged by size: 

                 680     184      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     756     906 
   Size:        756     150
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     366     591     906 
   Size:        366     225     315

  Fragments arranged by size: 

                 366     315     225
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     813     906 
   Size:        813      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     243     304     906 
   Size:        243      61     602

  Fragments arranged by size: 

                 602     243      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     538     631     638     639     648     660     661     771 
   Size:        538      93       7       1       9      12       1     110

Cuts at:    771     906 
   Size:        135

  Fragments arranged by size: 

                 538     135     110      93      12       9       7       1
                   1
 
HaeII r_GCGC'y

Cuts at:      0     406     906 
   Size:        406     500
 
HincII GTy'rAC

Cuts at:      0     531     906 
   Size:        531     375
 
KasI G'GCGC_C

Cuts at:      0     402     906 
   Size:        402     504
 
MscI TGG'CCA

Cuts at:      0     793     906 
   Size:        793     113
 
MspA1I CmG'CkG

Cuts at:      0     186     607     739     841     906 
   Size:        186     421     132     102      65

  Fragments arranged by size: 

                 421     186     132     102      65
 
NarI GG'CG_CC

Cuts at:      0     403     906 
   Size:        403     503
 
NcoI C'CATG_G

Cuts at:      0     590     906 
   Size:        590     316
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     634     906 
   Size:        634     272
 
PpuMI rG'GwC_Cy

Cuts at:      0     771     906 
   Size:        771     135
 
PspOMI G'GGCC_C

Cuts at:      0     638     660     906 
   Size:        638      22     246

  Fragments arranged by size: 

                 638     246      22
 
PspXI vC'TCGA_Gb

Cuts at:      0     783     906 
   Size:        783     123
 
PstI C_TGCA'G

Cuts at:      0     647     906 
   Size:        647     259
 
SacII CC_GC'GG

Cuts at:      0     608     740     906 
   Size:        608     132     166

  Fragments arranged by size: 

                 608     166     132
 
SanDI GG'GwC_CC

Cuts at:      0     771     906 
   Size:        771     135
 
SbfI CC_TGCA'GG

Cuts at:      0     647     906 
   Size:        647     259
 
SfcI C'TryA_G

Cuts at:      0     220     643     906 
   Size:        220     423     263

  Fragments arranged by size: 

                 423     263     220
 
SfoI GGC'GCC

Cuts at:      0     404     906 
   Size:        404     502
 
SmaI CCC'GGG

Cuts at:      0     617     669     906 
   Size:        617      52     237

  Fragments arranged by size: 

                 617     237      52
 
SmlI C'TyrA_G

Cuts at:      0     783     906 
   Size:        783     123
 
StuI AGG'CCT

Cuts at:      0     130     906 
   Size:        130     776
 
StyI C'CwwG_G

Cuts at:      0     473     590     906 
   Size:        473     117     316

  Fragments arranged by size: 

                 473     316     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     516     585     906 
   Size:        516      69     321

  Fragments arranged by size: 

                 516     321      69
 
XhoI C'TCGA_G

Cuts at:      0     783     906 
   Size:        783     123
 
XmaI C'CCGG_G

Cuts at:      0     615     667     906 
   Size:        615      52     239

  Fragments arranged by size: 

                 615     239      52
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI    ApaLI     AscI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BglI     BlpI Bme1580I    BsaHI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BsrBI    BsrFI   BssHII
     BtgI    BtgZI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII   HincII     KasI     MscI   MspA1I     NarI     NcoI
    PflMI    PpuMI   PspOMI    PspXI     PstI    SacII    SanDI     SbfI
     SfcI     SfoI     SmaI     SmlI     StuI     StyI    TaqII     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI     BsgI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com