Restriction map for pF1KB7605
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AseI           43  ATTAAT               AT^TAAT
AsnI           43  ATTAAT               AT^TAAT
BanII         214  GAGCTC               G[GA]GC[CT]^C
BclI          582  TGATCA               T^GATCA
BfaI          553  CTAG                 C^TAG
BglII         858  AGATCT               A^GATCT
BpmI          706  CTGGAG               CTGGAG(16/14)
BseRI         709  GAGGAG               GAGGAG(10/8)
BsgI          760  CTGCAC               GTGCAG(16/14)
BsiYI         447  CCTAAACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          447  CCTAAACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstF5I        537  GGATG                GGATG(2/0)
BstYI         858  AGATCT               [GA]^GATC[CT]
Cac8I         125  GCAGGC               GC[ACGT]^[ACGT]GC
EarI          475  GAAGAG               CTCTTC(1/4)
EcoNI         447  CCTAAACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          582  TGATCA               T^GATCA
FokI          537  GGATG                GGATG(9/13)
HphI          146  TCACC                GGTGA(8/7)
Ksp632I       475  GAAGAG               CTCTTC(1/4)
MaeI          553  CTAG                 C^TAG
MflI          858  AGATCT               [GA]^GATC[CT]
MslI           28  CATCAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          523  GCTACAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI           83  GACTC                GAGTC(4/5)
PshBI          43  ATTAAT               AT^TAAT
SacI          214  GAGCTC               GAGCT^C
TaqI          813  TCGA                 T^CGA
TthHB8I       813  TCGA                 T^CGA
XhoII         858  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflII         286  CTTAAG               C^TTAAG
              621  CTTAAG              
AspHI          22  GAGCAC               G[AT]GC[AT]^C
              214  GAGCTC              
BbsI          504  GAAGAC               GAAGAC(2/6)
              669  GAAGAC              
BfrI          286  CTTAAG               C^TTAAG
              621  CTTAAG              
BmyI           22  GAGCAC               G[AGT]GC[ACT]^C
              214  GAGCTC              
BpuAI         504  GAAGAC               GAAGAC(2/6)
              669  GAAGAC              
BsiHKAI        22  GAGCAC               G[AT]GC[AT]^C
              214  GAGCTC              
BsmAI          81  GAGAC                GTCTC(1/5)
              160  GTCTC               
Bsp1286I       22  GAGCAC               G[AGT]GC[ACT]^C
              214  GAGCTC              
Cfr13I         12  GGGCC                G^G[ACGT]CC
              612  GGCCC               
HaeIII         13  GGCC                 GG^CC
              612  GGCC                
MspA1I        256  CAGCTG               C[AC]G^C[GT]G
              757  CAGCTG              
NlaIII        765  CATG                 CATG^
              819  CATG                
PvuII         256  CAGCTG               CAG^CTG
              757  CAGCTG              
Sau96I         12  GGGCC                G^G[ACGT]CC
              612  GGCCC               
SfcI           92  CTGTAG               C^T[GA][CT]AG
              292  CTACAG              
Tsp45I        397  GTCAC                ^GT[GC]AC
              702  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BsrI        
DdeI        DpnII       Fnu4HI      HinfI       ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
NdeII       Sau3AI      SmlI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       
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