Restriction map for pF1KB7586
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstYI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HinfI       HpaI        KpnI        KspI        MaeI        
MamI        MboI        MfeI        MflI        MluI        
MunI        NaeI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PvuI        RcaI        RsaI        RsrII       
SacI        SacII       Sau3AI      SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tth111I     
XbaI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI           55  GTCGAC               GT^[AC][GT]AC
AccIII          9  TCCGGA               T^CCGGA
AclI          307  AACGTT               AA^CGTT
AcsI          811  GAATTT               [GA]^AATT[CT]
AlwNI         439  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          811  GAATTT               [GA]^AATT[CT]
AseI          585  ATTAAT               AT^TAAT
AsnI          585  ATTAAT               AT^TAAT
AvaI          280  CTCGAG               C^[CT]CG[GA]G
BanII         191  GAGCCC               G[GA]GC[CT]^C
BbvCI         646  GCTGAGG              CCTCAGC(-5/-2)
BlpI           42  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       42  GCTCAGC              GC^T[ACGT]AGC
BsaI          275  GAGACC               GGTCTC(1/5)
BsaWI           9  TCCGGA               [AT]^CCGG[AT]
BseAI           9  TCCGGA               T^CCGGA
BsgI          532  GTGCAG               GTGCAG(16/14)
BsmFI         701  GGGAC                GGGAC(10/14)
BsmI           84  GAATGC               GAATGC(1/-1)
BsoBI         280  CTCGAG               C^[CT]CG[GA]G
BspEI           9  TCCGGA               T^CCGGA
BsrDI         428  CATTGC               GCAATG(2/0)
BstEII        636  GGTAACC              G^GT[ACGT]ACC
BstPI         636  GGTAACC              G^GT[ACGT]ACC
CelII          42  GCTCAGC              GC^T[ACGT]AGC
EcoNI         148  CCTGAGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       636  GGTAACC              G^GT[ACGT]ACC
EcoT14I       195  CCAAGG               C^C[AT][AT]GG
HincII         55  GTCGAC               GT[CT]^[GA]AC
HindII         55  GTCGAC               GT[CT]^[GA]AC
HphI          422  TCACC                GGTGA(8/7)
MroI            9  TCCGGA               T^CCGGA
NspI          599  ACATGC               [GA]CATG^[CT]
PaeR7I        280  CTCGAG               C^TCGAG
PflMI         793  CCACCCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         585  ATTAAT               AT^TAAT
Psp1406I      307  AACGTT               AA^CGTT
SalI           55  GTCGAC               G^TCGAC
SapI          554  GAAGAGC              GCTCTTC(1/4)
SfaNI         133  GATGC                GCATC(5/9)
SmlI          280  CTCGAG               C^T[CT][GA]AG
StyI          195  CCAAGG               C^C[AT][AT]GG
Tsp45I        517  GTGAC                ^GT[GC]AC
Tsp509I       812  AATT                 ^AATT
Van91I        793  CCACCCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          804  CCAGGCTGAATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          280  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI          244  GGCGCC               G[GA]^CG[CT]C
              367  GGCGCC              
AvaII          12  GGACC                G^G[AT]CC
              702  GGACC               
BalI          258  TGGCCA               TGG^CCA
              801  TGGCCA              
BbeI          244  GGCGCC               GGCGC^C
              367  GGCGCC              
BglI          359  GCCCACAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              652  GCCATAACGGC         
BmyI          191  GAGCCC               G[AGT]GC[ACT]^C
              285  GGGCAC              
BpmI          382  CTGGAG               CTGGAG(16/14)
              445  CTGGAG              
BsaHI         244  GGCGCC               G[GA]^CG[CT]C
              367  GGCGCC              
BsaJI         195  CCAAGG               C^C[ACGT][ACGT]GG
              340  CCTGGG              
BsmAI         275  GAGAC                GTCTC(1/5)
              570  GAGAC               
Bsp1286I      191  GAGCCC               G[AGT]GC[ACT]^C
              285  GGGCAC              
BstF5I        132  GGATG                GGATG(2/0)
              680  GGATG               
EaeI          258  TGGCCA               [CT]^GGCC[GA]
              801  TGGCCA              
FauI          796  CCCGC                CCCGC(4/6)
              677  GCGGG               
FokI          132  GGATG                GGATG(9/13)
              680  GGATG               
HaeII         244  GGCGCC               [GA]GCGC^[CT]
              367  GGCGCC              
HapII          10  CCGG                 C^CGG
              248  CCGG                
Hin1I         244  GGCGCC               G[GA]^CG[CT]C
              367  GGCGCC              
HpaII          10  CCGG                 C^CGG
              248  CCGG                
KasI          244  GGCGCC               G^GCGCC
              367  GGCGCC              
MaeII         308  ACGT                 A^CGT
              410  ACGT                
MaeIII        517  GTGAC                ^GT[ACGT]AC
              637  GTAAC               
MluNI         258  TGGCCA               TGG^CCA
              801  TGGCCA              
MscI          258  TGGCCA               TGG^CCA
              801  TGGCCA              
MseI          178  TTAA                 T^TAA
              586  TTAA                
MslI          510  CATGAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              612  CATGGCAGTG          
MspI           10  CCGG                 C^CGG
              248  CCGG                
NarI          244  GGCGCC               GG^CGCC
              367  GGCGCC              
PstI           50  CTGCAG               CTGCA^G
              436  CTGCAG              
PvuII         433  CAGCTG               CAG^CTG
              644  CAGCTG              
SfoI          244  GGCGCC               GGC^GCC
              367  GGCGCC              
TaqI           56  TCGA                 T^CGA
              281  TCGA                
Tru9I         178  TTAA                 T^TAA
              586  TTAA                
TthHB8I        56  TCGA                 T^CGA
              281  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AccII       AciI        AluI        BanI        BbvI        
BseRI       BsiYI       BslI        BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        EarI        EcoRII      Fnu4HI      HaeIII      
HhaI        HinP1I      ItaI        Ksp632I     MboII       
MnlI        MspA1I      MvaI        MvnI        MwoI        
NlaIII      NlaIV       Sau96I      ScrFI       SfcI        
TseI        TspRI       
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