Restriction map for pF1KB7586
 (Linear) (Six-base) MAPSORT of: KIBB7586.seq  Check: 1063  from: 1  to: 864



KIBB7586.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0      56     864 
   Size:         56     808
 
AclI AA'CG_TT

Cuts at:      0     308     864 
   Size:        308     556
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     864 
   Size:        128     736
 
AlwNI CAG_nnn'CTG

Cuts at:      0     444     864 
   Size:        444     420
 
ApoI r'AATT_y

Cuts at:      0     811     864 
   Size:        811      53
 
AseI AT'TA_AT

Cuts at:      0     586     864 
   Size:        586     278
 
AvaI C'yCGr_G

Cuts at:      0     280     864 
   Size:        280     584
 
BanI G'GyrC_C

Cuts at:      0     244     286     367     660     864 
   Size:        244      42      81     293     204

  Fragments arranged by size: 

                 293     244     204      81      42
 
BanII G_rGCy'C

Cuts at:      0     195     864 
   Size:        195     669
 
BbeI G_GCGC'C

Cuts at:      0     248     371     864 
   Size:        248     123     493

  Fragments arranged by size: 

                 493     248     123
 
BbvCI CC'TCA_GC

Cuts at:      0     647     864 
   Size:        647     217
 
BglI GCCn_nnn'nGGC

Cuts at:      0     365     658     864 
   Size:        365     293     206

  Fragments arranged by size: 

                 365     293     206
 
BlpI GC'TnA_GC

Cuts at:      0      43     864 
   Size:         43     821
 
Bme1580I G_kGCm'C

Cuts at:      0     289     864 
   Size:        289     575
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     470     864 
   Size:        470     394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     466     864 
   Size:        403      63     398

  Fragments arranged by size: 

                 403     398      63
 
Bpu10I CC'TnA_GC

Cuts at:      0     143     149     647     864 
   Size:        143       6     498     217

  Fragments arranged by size: 

                 498     217     143       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     269     864 
   Size:        269     595
 
BsaHI Gr'CG_yC

Cuts at:      0     245     368     864 
   Size:        245     123     496

  Fragments arranged by size: 

                 496     245     123
 
BsaWI w'CCGG_w

Cuts at:      0       9     864 
   Size:          9     855
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     582     740     864 
   Size:        136     446     158     124

  Fragments arranged by size: 

                 446     158     136     124
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     864 
   Size:        553     311
 
BsmI GAATG_Cn'

Cuts at:      0      90     864 
   Size:         90     774
 
Bsp1286I G_dGCh'C

Cuts at:      0     195     289     864 
   Size:        195      94     575

  Fragments arranged by size: 

                 575     195      94
 
BspEI T'CCGG_A

Cuts at:      0       9     864 
   Size:          9     855
 
BsrDI GCAATG_nn'

Cuts at:      0     427     864 
   Size:        427     437
 
BstEII G'GTnAC_C

Cuts at:      0     636     864 
   Size:        636     228
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     314     864 
   Size:        314     550
 
BtsI GCAGTG_nn'

Cuts at:      0     536     623     864 
   Size:        536      87     241

  Fragments arranged by size: 

                 536     241      87
 
EaeI y'GGCC_r

Cuts at:      0     258     801     864 
   Size:        258     543      63

  Fragments arranged by size: 

                 543     258      63
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     122     549     864 
   Size:        104      18     427     315

  Fragments arranged by size: 

                 427     315     104      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     215     236     864 
   Size:        215      21     628

  Fragments arranged by size: 

                 628     215      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     403     466     864 
   Size:        128     275      63     398

  Fragments arranged by size: 

                 398     275     128      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     152     864 
   Size:        152     712
 
HaeII r_GCGC'y

Cuts at:      0     248     371     864 
   Size:        248     123     493

  Fragments arranged by size: 

                 493     248     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     558     590     864 
   Size:        558      32     274

  Fragments arranged by size: 

                 558     274      32
 
HincII GTy'rAC

Cuts at:      0      57     864 
   Size:         57     807
 
KasI G'GCGC_C

Cuts at:      0     244     367     864 
   Size:        244     123     497

  Fragments arranged by size: 

                 497     244     123
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     483     864 
   Size:        483     381
 
MscI TGG'CCA

Cuts at:      0     260     803     864 
   Size:        260     543      61

  Fragments arranged by size: 

                 543     260      61
 
MslI CAynn'nnrTG

Cuts at:      0     514     616     864 
   Size:        514     102     248

  Fragments arranged by size: 

                 514     248     102
 
MspA1I CmG'CkG

Cuts at:      0     293     435     646     677     799     864 
   Size:        293     142     211      31     122      65

  Fragments arranged by size: 

                 293     211     142     122      65      31
 
NarI GG'CG_CC

Cuts at:      0     245     368     864 
   Size:        245     123     496

  Fragments arranged by size: 

                 496     245     123
 
NspI r_CATG'y

Cuts at:      0     603     864 
   Size:        603     261
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     799     864 
   Size:        799      65
 
PspXI vC'TCGA_Gb

Cuts at:      0     280     864 
   Size:        280     584
 
PstI C_TGCA'G

Cuts at:      0      54     440     864 
   Size:         54     386     424

  Fragments arranged by size: 

                 424     386      54
 
PvuII CAG'CTG

Cuts at:      0     435     646     864 
   Size:        435     211     218

  Fragments arranged by size: 

                 435     218     211
 
SalI G'TCGA_C

Cuts at:      0      55     864 
   Size:         55     809
 
SapI GCTCTTCn'nnn_

Cuts at:      0     549     864 
   Size:        549     315
 
SfcI C'TryA_G

Cuts at:      0      50      73     436     864 
   Size:         50      23     363     428

  Fragments arranged by size: 

                 428     363      50      23
 
SfoI GGC'GCC

Cuts at:      0     246     369     864 
   Size:        246     123     495

  Fragments arranged by size: 

                 495     246     123
 
SmlI C'TyrA_G

Cuts at:      0     280     864 
   Size:        280     584
 
StyI C'CwwG_G

Cuts at:      0     195     864 
   Size:        195     669
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     697     729     864 
   Size:        697      32     135

  Fragments arranged by size: 

                 697     135      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     811     864 
   Size:        811      53
 
XhoI C'TCGA_G

Cuts at:      0     280     864 
   Size:        280     584
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AlwNI     ApoI     AseI     AvaI     BanI
    BanII     BbeI    BbvCI     BglI     BlpI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI    BseRI     BsgI     BsmI Bsp1286I
    BspEI    BsrDI   BstEII    BtgZI     BtsI     EaeI     EarI     EciI
  Eco57MI    EcoNI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NspI    PflMI    PspXI     PstI    PvuII
     SalI     SapI     SfcI     SfoI     SmlI     StyI     TstI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaXI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XmaI     XmnI     ZraI
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