Restriction map for pF1KB7564
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AlwNI       AocI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BalI        BamHI       BanI        
BbeI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        SapI        SbfI        ScaI        SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         183  CGCG                 CG^CG
AcyI          256  GACGCC               G[GA]^CG[CT]C
AfaI          687  GTAC                 GT^AC
Alw44I        444  GTGCAC               G^TGCAC
Aor51HI       557  AGCGCT               AGC^GCT
ApaLI         444  GTGCAC               G^TGCAC
AvrII         105  CCTAGG               C^CTAGG
BbsI           10  GTCTTC               GAAGAC(2/6)
BfaI          106  CTAG                 C^TAG
BlnI          105  CCTAGG               C^CTAGG
BpuAI          10  GTCTTC               GAAGAC(2/6)
BsaHI         256  GACGCC               G[GA]^CG[CT]C
BsaI           39  GGTCTC               GGTCTC(1/5)
BsiEI         299  CGGTCG               CG[GA][CT]^CG
BsmAI          40  GTCTC                GTCTC(1/5)
BsmI          512  GCATTC               GAATGC(1/-1)
BspMI         689  ACCTGC               ACCTGC(4/8)
BstUI         183  CGCG                 CG^CG
CpoI           44  CGGTCCG              CG^G[AT]CCG
DdeI          802  CTGAG                C^T[ACGT]AG
DpnII         308  GATC                 ^GATC
DsaI          591  CCATGG               C^C[GA][CT]GG
Eco47III      557  AGCGCT               AGC^GCT
HgaI          256  GACGC                GACGC(5/10)
Hin1I         256  GACGCC               G[GA]^CG[CT]C
MaeI          106  CTAG                 C^TAG
MboI          308  GATC                 ^GATC
MvnI          183  CGCG                 CG^CG
NcoI          591  CCATGG               C^CATGG
NdeII         308  GATC                 ^GATC
NspI          395  GCATGC               [GA]CATG^[CT]
PpuMI         680  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI          687  GTAC                 GT^AC
RsrII          44  CGGTCCG              CG^G[AT]CCG
SacI           61  GAGCTC               GAGCT^C
Sau3AI        308  GATC                 ^GATC
SexAI         425  ACCAGGT              A^CC[AT]GGT
SmlI          412  CTCAAG               C^T[CT][GA]AG
SphI          395  GCATGC               GCATG^C
XcmI            5  CCAAAGTCTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

ApaI          264  GGGCCC               GGGCC^C
              666  GGGCCC              
AspHI          61  GAGCTC               G[AT]GC[AT]^C
              444  GTGCAC              
AvaII          45  GGTCC                G^G[AT]CC
              681  GGACC               
BbrPI         333  CACGTG               CAC^GTG
              694  CACGTG              
BpmI          194  CTGGAG               CTGGAG(16/14)
              568  CTGGAG              
BsaAI         333  CACGTG               [CT]AC^GT[GA]
              694  CACGTG              
BsiHKAI        61  GAGCTC               G[AT]GC[AT]^C
              444  GTGCAC              
BsrI          747  ACTGG                ACTGG(1/-1)
               97  CCAGT               
EarI           21  GAAGAG               CTCTTC(1/4)
              486  GAAGAG              
EcoNI         756  CCTTCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              801  CCTGAGTGAGG         
EcoT14I       105  CCTAGG               C^C[AT][AT]GG
              591  CCATGG              
HaeII         293  AGCGCC               [GA]GCGC^[CT]
              557  AGCGCT              
HinfI         343  GACTC                G^A[ACGT]TC
              571  GAGTC               
Ksp632I        21  GAAGAG               CTCTTC(1/4)
              486  GAAGAG              
MaeIII        362  GTGAC                ^GT[ACGT]AC
              407  GTCAC               
MslI           93  CATCCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              355  CACAGCTGTG          
NlaIII        396  CATG                 CATG^
              592  CATG                
PleI          343  GACTC                GAGTC(4/5)
              571  GAGTC               
PmaCI         333  CACGTG               CAC^GTG
              694  CACGTG              
PmlI          333  CACGTG               CAC^GTG
              694  CACGTG              
PstI          388  CTGCAG               CTGCA^G
              760  CTGCAG              
PvuII          51  CAGCTG               CAG^CTG
              357  CAGCTG              
SfcI          388  CTGCAG               C^T[GA][CT]AG
              760  CTGCAG              
StyI          105  CCTAGG               C^C[AT][AT]GG
              591  CCATGG              
TaqI          310  TCGA                 T^CGA
              479  TCGA                
Tsp45I        362  GTGAC                ^GT[GC]AC
              407  GTCAC               
TspRI          98  CAGTG                CA[GC]TG[ACGT][ACGT]^
              702  CAGTG               
TthHB8I       310  TCGA                 T^CGA
              479  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AvaI        BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeII       MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIV       Sau96I      
ScrFI       TseI        
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