Restriction map for pF1KB7563
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HapII       HincII      HindII      HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
KspI        MamI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       StuI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcsI          436  AAATTC               [GA]^AATT[CT]
AcyI           91  GACGCC               G[GA]^CG[CT]C
ApoI          436  AAATTC               [GA]^AATT[CT]
AvaI           48  CTCGGG               C^[CT]CG[GA]G
AvaII         559  GGACC                G^G[AT]CC
BsaHI          91  GACGCC               G[GA]^CG[CT]C
BseRI         236  GAGGAG               GAGGAG(10/8)
BsiWI          11  CGTACG               C^GTACG
BsmAI         445  GAGAC                GTCTC(1/5)
BsmFI         185  GGGAC                GGGAC(10/14)
BsoBI          48  CTCGGG               C^[CT]CG[GA]G
BssKI         354  CCTGG                ^CC[ACGT]GG
BssSI         497  CACGAG               CACGAG(-5/-1)
BstAPI         59  GCAAGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         354  CCTGG                CC^[AT]GG
CfoI          452  GCGC                 GCG^C
Cfr13I        559  GGACC                G^G[ACGT]CC
DdeI           84  CTCAG                C^T[ACGT]AG
DraI          133  TTTAAA               TTT^AAA
DsaI          242  CCATGG               C^C[GA][CT]GG
EcoRII        354  CCTGG                ^CC[AT]GG
EcoT14I       242  CCATGG               C^C[AT][AT]GG
FauI          193  GCGGG                CCCGC(4/6)
HgaI           91  GACGC                GACGC(5/10)
HhaI          452  GCGC                 GCG^C
Hin1I          91  GACGCC               G[GA]^CG[CT]C
HinP1I        452  GCGC                 G^CGC
HindIII       172  AAGCTT               A^AGCTT
MaeII          10  ACGT                 A^CGT
MaeIII        359  GTAAC                ^GT[ACGT]AC
MfeI          419  CAATTG               C^AATTG
MspA1I        191  CAGCGG               C[AC]G^C[GT]G
MunI          419  CAATTG               C^AATTG
MvaI          354  CCTGG                CC^[AT]GG
MwoI           59  GCAAGACCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          242  CCATGG               C^CATGG
NheI          414  GCTAGC               G^CTAGC
NlaIV         238  GGAGCC               GG[ACGT]^[ACGT]CC
NspI          324  GCATGC               [GA]CATG^[CT]
PleI           46  GACTC                GAGTC(4/5)
PshAI         386  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          319  GAAGAGC              GCTCTTC(1/4)
Sau96I        559  GGACC                G^G[ACGT]CC
ScrFI         354  CCTGG                CC^[ACGT]GG
SfcI          231  CTACAG               C^T[GA][CT]AG
SphI          324  GCATGC               GCATG^C
SspI          292  AATATT               AAT^ATT
StyI          242  CCATGG               C^C[AT][AT]GG
TaqI          341  TCGA                 T^CGA
TfiI          206  GATTC                G^A[AT]TC
TthHB8I       341  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           78  CCGC                 CCGC(-3/-1)
              193  GCGG                
AfaI           12  GTAC                 GT^AC
              228  GTAC                
AlwI          351  GATCC                GGATC(4/5)
              305  GGATC               
BbsI            6  GAAGAC               GAAGAC(2/6)
              591  GAAGAC              
BfaI          154  CTAG                 C^TAG
              415  CTAG                
BpuAI           6  GAAGAC               GAAGAC(2/6)
              591  GAAGAC              
BsaJI         242  CCATGG               C^C[ACGT][ACGT]GG
              354  CCTGGG              
BspMI          64  ACCTGC               ACCTGC(4/8)
              553  GCAGGT              
HinfI          46  GACTC                G^A[ACGT]TC
              206  GATTC               
MaeI          154  CTAG                 C^TAG
              415  CTAG                
NlaIII        243  CATG                 CATG^
              325  CATG                
RsaI           12  GTAC                 GT^AC
              228  GTAC                
TspRI         462  CAGTG                CA[GC]TG[ACGT][ACGT]^
              563  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BsrI        Cac8I       DpnII       EarI        
Ksp632I     MboI        MboII       MnlI        MseI        
NdeII       Sau3AI      Tru9I       Tsp509I     
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