Restriction map for pF1KB7559
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AlwNI       
AocI        ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BsaBI       BsaHI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MamI        MfeI        
MflI        MluI        MseI        MslI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccIII         24  TCCGGA               T^CCGGA
AfaI           60  GTAC                 GT^AC
AhdI          127  GACAACTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        517  GTGCAC               G^TGCAC
AlwI           22  GATCC                GGATC(4/5)
Aor51HI       170  AGCGCT               AGC^GCT
ApaLI         517  GTGCAC               G^TGCAC
AspEI         127  GACAACTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         517  GTGCAC               G[AT]GC[AT]^C
AvaI          552  CTCGGG               C^[CT]CG[GA]G
BalI          538  TGGCCA               TGG^CCA
BanI          408  GGTGCC               G^G[CT][GA]CC
BbsI          557  GAAGAC               GAAGAC(2/6)
BpmI          381  CTCCAG               CTGGAG(16/14)
BpuAI         557  GAAGAC               GAAGAC(2/6)
BsaAI         514  TACGTG               [CT]AC^GT[GA]
BsaI          549  GGTCTC               GGTCTC(1/5)
BseAI          24  TCCGGA               T^CCGGA
BsiHKAI       517  GTGCAC               G[AT]GC[AT]^C
BsmAI         550  GTCTC                GTCTC(1/5)
BsmFI          67  GTCCC                GGGAC(10/14)
BsoBI         552  CTCGGG               C^[CT]CG[GA]G
BspEI          24  TCCGGA               T^CCGGA
BsrFI         190  GCCGGC               [GA]^CCGG[CT]
BstAPI        480  GCAGCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        356  GGATG                GGATG(2/0)
Cfr10I        190  GCCGGC               [GA]^CCGG[CT]
DpnII          22  GATC                 ^GATC
DraII         149  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        520  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      127  GACAACTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      170  AGCGCT               AGC^GCT
EcoO109I      149  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       290  CCAAGG               C^C[AT][AT]GG
FokI          356  GGATG                GGATG(9/13)
HaeII         170  AGCGCT               [GA]GCGC^[CT]
HinfI         565  GACTC                G^A[ACGT]TC
KspI           93  CCGCGG               CCGC^GG
MaeII         515  ACGT                 A^CGT
MboI           22  GATC                 ^GATC
MboII         557  GAAGA                GAAGA(8/7)
MluNI         538  TGGCCA               TGG^CCA
MroI           24  TCCGGA               T^CCGGA
MscI          538  TGGCCA               TGG^CCA
NaeI          190  GCCGGC               GCC^GGC
NdeII          22  GATC                 ^GATC
NgoMIV        190  GCCGGC               G^CCGGC
NlaIII        536  CATG                 CATG^
PleI          565  GACTC                GAGTC(4/5)
PpuMI         149  AGGACCC              [GA]G^G[AT]CC[CT]
PstI           49  CTGCAG               CTGCA^G
PvuII         481  CAGCTG               CAG^CTG
RsaI           60  GTAC                 GT^AC
SacII          93  CCGCGG               CCGC^GG
Sau3AI         22  GATC                 ^GATC
SfaNI         357  GATGC                GCATC(5/9)
SfcI           49  CTGCAG               C^T[GA][CT]AG
SmlI          388  CTCAAG               C^T[CT][GA]AG
StyI          290  CCAAGG               C^C[AT][AT]GG
Tsp45I        125  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BanII           9  GGGCTC               G[GA]GC[CT]^C
              294  GGGCTC              
BlpI          296  GCTCAGC              GC^T[ACGT]AGC
              366  GCTGAGC             
Bpu1102I      296  GCTCAGC              GC^T[ACGT]AGC
              366  GCTGAGC             
BsaWI          24  TCCGGA               [AT]^CCGG[AT]
              561  ACCGGA              
BspMI         473  ACCTGC               ACCTGC(4/8)
              247  GCAGGT              
BstNI         383  CCAGG                CC^[AT]GG
              404  CCTGG               
CelII         296  GCTCAGC              GC^T[ACGT]AGC
              366  GCTGAGC             
DsaI            5  CCACGG               C^C[GA][CT]GG
               93  CCGCGG              
EcoNI          74  CCTCCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              377  CCTTCTCCAGG         
EcoRII        383  CCAGG                ^CC[AT]GG
              404  CCTGG               
HphI           15  GGTGA                GGTGA(8/7)
              471  TCACC               
MaeIII        125  GTGAC                ^GT[ACGT]AC
              461  GTTAC               
MvaI          383  CCAGG                CC^[AT]GG
              404  CCTGG               
TspRI         123  CAGTG                CA[GC]TG[ACGT][ACGT]^
              524  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        EaeI        
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MnlI        MspA1I      
MspI        MvnI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       TseI        
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