Restriction map for pF1KB7559
 (Linear) (Six-base) MAPSORT of: KIBB7559.seq  Check: 1641  from: 1  to: 618



KIBB7559.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     238     482     618 
   Size:        238     244     136

  Fragments arranged by size: 

                 244     238     136
 
AfeI AGC'GCT

Cuts at:      0     172     618 
   Size:        172     446
 
AhdI GACnn_n'nnGTC

Cuts at:      0     132     618 
   Size:        132     486
 
ApaLI G'TGCA_C

Cuts at:      0     517     618 
   Size:        517     101
 
AvaI C'yCGr_G

Cuts at:      0     552     618 
   Size:        552      66
 
BanI G'GyrC_C

Cuts at:      0     408     618 
   Size:        408     210
 
BanII G_rGCy'C

Cuts at:      0      13     298     618 
   Size:         13     285     320

  Fragments arranged by size: 

                 320     285      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     564     618 
   Size:        564      54
 
BlpI GC'TnA_GC

Cuts at:      0     297     367     618 
   Size:        297      70     251

  Fragments arranged by size: 

                 297     251      70
 
Bme1580I G_kGCm'C

Cuts at:      0     187     354     413     521     618 
   Size:        187     167      59     108      97

  Fragments arranged by size: 

                 187     167     108      97      59
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116     517     618 
   Size:        116     401     101

  Fragments arranged by size: 

                 401     116     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     618 
   Size:        366     252
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     618 
   Size:        373     245
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     555     618 
   Size:        555      63
 
BsaAI yAC'GTr

Cuts at:      0     516     618 
   Size:        516     102
 
BsaWI w'CCGG_w

Cuts at:      0      24     561     618 
   Size:         24     537      57

  Fragments arranged by size: 

                 537      57      24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     157     160     178     181     618 
   Size:         43     114       3      18       3     437

  Fragments arranged by size: 

                 437     114      43      18       3       3
 
BseYI C'CCAG_C

Cuts at:      0     232     618 
   Size:        232     386
 
BsiHKAI G_wGCw'C

Cuts at:      0     521     618 
   Size:        521      97
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     187     298     354     413     521     618 
   Size:         13     174     111      56      59     108      97

  Fragments arranged by size: 

                 174     111     108      97      59      56      13
 
BspEI T'CCGG_A

Cuts at:      0      24     618 
   Size:         24     594
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     238     482     618 
   Size:        238     244     136

  Fragments arranged by size: 

                 244     238     136
 
BsrFI r'CCGG_y

Cuts at:      0     190     618 
   Size:        190     428
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     486     618 
   Size:        486     132
 
BtgI C'CryG_G

Cuts at:      0       5      93     618 
   Size:          5      88     525

  Fragments arranged by size: 

                 525      88       5
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     450     618 
   Size:        450     168
 
DraIII CAC_nnn'GTG

Cuts at:      0     525     618 
   Size:        525      93
 
EaeI y'GGCC_r

Cuts at:      0     287     332     538     618 
   Size:        287      45     206      80

  Fragments arranged by size: 

                 287     206      80      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     618 
   Size:        366     252
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      78     381     618 
   Size:         78     303     237

  Fragments arranged by size: 

                 303     237      78
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     618 
   Size:        150     468
 
HaeII r_GCGC'y

Cuts at:      0     174     618 
   Size:        174     444
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     618 
   Size:        118      32     468

  Fragments arranged by size: 

                 468     118      32
 
MscI TGG'CCA

Cuts at:      0     540     618 
   Size:        540      78
 
MspA1I CmG'CkG

Cuts at:      0      54      95     324     483     618 
   Size:         54      41     229     159     135

  Fragments arranged by size: 

                 229     159     135      54      41
 
NaeI GCC'GGC

Cuts at:      0     192     618 
   Size:        192     426
 
NgoMIV G'CCGG_C

Cuts at:      0     190     618 
   Size:        190     428
 
PasI CC'CwG_GG

Cuts at:      0     404     618 
   Size:        404     214
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     618 
   Size:        150     468
 
PstI C_TGCA'G

Cuts at:      0      53     618 
   Size:         53     565
 
PvuII CAG'CTG

Cuts at:      0     483     618 
   Size:        483     135
 
SacII CC_GC'GG

Cuts at:      0      96     618 
   Size:         96     522
 
SfcI C'TryA_G

Cuts at:      0      49     618 
   Size:         49     569
 
SmlI C'TyrA_G

Cuts at:      0     388     618 
   Size:        388     230
 
StyI C'CwwG_G

Cuts at:      0     290     618 
   Size:        290     328
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     396     536     618 
   Size:        396     140      82

  Fragments arranged by size: 

                 396     140      82
 

 Enzymes that do cut:

     AarI     AfeI     AhdI    ApaLI     AvaI     BanI    BanII     BbsI
     BlpI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaWI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI   BstAPI
     BtgI    BtgZI   DraIII     EaeI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I     MscI   MspA1I     NaeI   NgoMIV     PasI    PpuMI     PstI
    PvuII    SacII     SfcI     SmlI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI   Bpu10I
    BsaBI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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