Restriction map for pF1KB7557
 (Linear) (Six-base) MAPSORT of: KIBB7557.seq  Check: 1793  from: 1  to: 606



KIBB7557.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     559     606 
   Size:        559      47
 
AclI AA'CG_TT

Cuts at:      0     145     606 
   Size:        145     461
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     413     606 
   Size:         91     322     193

  Fragments arranged by size: 

                 322     193      91
 
AleI CACnn'nnGTG

Cuts at:      0      49     606 
   Size:         49     557
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     422     606 
   Size:        422     184
 
ApaI G_GGCC'C

Cuts at:      0     487     606 
   Size:        487     119
 
ApoI r'AATT_y

Cuts at:      0     346     606 
   Size:        346     260
 
BanI G'GyrC_C

Cuts at:      0      43     606 
   Size:         43     563
 
BanII G_rGCy'C

Cuts at:      0     487     606 
   Size:        487     119
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     292     606 
   Size:        292     314
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     129     163     606 
   Size:        129      34     443

  Fragments arranged by size: 

                 443     129      34
 
BglII A'GATC_T

Cuts at:      0     112     606 
   Size:        112     494
 
Bme1580I G_kGCm'C

Cuts at:      0     487     606 
   Size:        487     119
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     451     606 
   Size:        451     155
 
Bpu10I CC'TnA_GC

Cuts at:      0      59     606 
   Size:         59     547
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     441     606 
   Size:        101     340     165

  Fragments arranged by size: 

                 340     165     101
 
BsaBI GATnn'nnATC

Cuts at:      0     329     606 
   Size:        329     277
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466     606 
   Size:        466     140
 
BseYI C'CCAG_C

Cuts at:      0      75     480     606 
   Size:         75     405     126

  Fragments arranged by size: 

                 405     126      75
 
BsiHKAI G_wGCw'C

Cuts at:      0     401     606 
   Size:        401     205
 
BsmI GAATG_Cn'

Cuts at:      0     551     606 
   Size:        551      55
 
Bsp1286I G_dGCh'C

Cuts at:      0     401     487     606 
   Size:        401      86     119

  Fragments arranged by size: 

                 401     119      86
 
BspHI T'CATG_A

Cuts at:      0     437     606 
   Size:        437     169
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     395     606 
   Size:        395     211
 
BstYI r'GATC_y

Cuts at:      0     112     606 
   Size:        112     494
 
BtgI C'CryG_G

Cuts at:      0     517     606 
   Size:        517      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     389     606 
   Size:        389     217
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     413     606 
   Size:         91     322     193

  Fragments arranged by size: 

                 322     193      91
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     448     606 
   Size:        448     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0     235     484     606 
   Size:        235     249     122

  Fragments arranged by size: 

                 249     235     122
 
FspI TGC'GCA

Cuts at:      0     410     606 
   Size:        410     196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      77      97     109     129     495     527     606 
   Size:         77      20      12      20     366      32      79

  Fragments arranged by size: 

                 366      79      77      32      20      20      12
 
HincII GTy'rAC

Cuts at:      0     201     560     606 
   Size:        201     359      46

  Fragments arranged by size: 

                 359     201      46
 
HindIII A'AGCT_T

Cuts at:      0     258     606 
   Size:        258     348
 
MslI CAynn'nnrTG

Cuts at:      0      49     606 
   Size:         49     557
 
MspA1I CmG'CkG

Cuts at:      0     480     606 
   Size:        480     126
 
NcoI C'CATG_G

Cuts at:      0     517     606 
   Size:        517      89
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     136     606 
   Size:        136     470
 
PpuMI rG'GwC_Cy

Cuts at:      0     235     606 
   Size:        235     371
 
PspOMI G'GGCC_C

Cuts at:      0     483     606 
   Size:        483     123
 
PvuII CAG'CTG

Cuts at:      0     480     606 
   Size:        480     126
 
SalI G'TCGA_C

Cuts at:      0     558     606 
   Size:        558      48
 
SanDI GG'GwC_CC

Cuts at:      0     235     606 
   Size:        235     371
 
SapI GCTCTTCn'nnn_

Cuts at:      0     389     606 
   Size:        389     217
 
SfcI C'TryA_G

Cuts at:      0     209     606 
   Size:        209     397
 
SmlI C'TyrA_G

Cuts at:      0     116     420     606 
   Size:        116     304     186

  Fragments arranged by size: 

                 304     186     116
 
StyI C'CwwG_G

Cuts at:      0     230     517     606 
   Size:        230     287      89

  Fragments arranged by size: 

                 287     230      89
 
XbaI T'CTAG_A

Cuts at:      0     366     606 
   Size:        366     240
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AleI     AlfI     ApaI     ApoI     BanI
    BanII     BbsI     BcgI    BglII Bme1580I     BplI   Bpu10I    BpuEI
    BsaBI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BstYI     BtgI     EarI  Eco57MI    EcoNI EcoO109I     FspI    Hin4I
   HincII  HindIII     MslI   MspA1I     NcoI    PflMI    PpuMI   PspOMI
    PvuII     SalI    SanDI     SapI     SfcI     SmlI     StyI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BpmI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI
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