Restriction map for pF1KB7552
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbrPI       BbsI        BcgI        
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FokI        FseI        FspI        
HgaI        HincII      HindII      HpaI        HphI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MseI        MunI        NaeI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII           5  CGCG                 CG^CG
AccIII        229  TCCGGA               T^CCGGA
AcyI          514  GGCGCC               G[GA]^CG[CT]C
AlwI          138  GATCC                GGATC(4/5)
AlwNI         585  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          428  TGGCCA               TGG^CCA
BanI          514  GGCGCC               G^G[CT][GA]CC
BanII         686  GGGCTC               G[GA]GC[CT]^C
BbeI          514  GGCGCC               GGCGC^C
BbvCI         141  CCTCAGC              CCTCAGC(-5/-2)
BciVI         232  GGATAC               GTATCC(6/5)
BfaI          464  CTAG                 C^TAG
BglII         563  AGATCT               A^GATCT
BmyI          686  GGGCTC               G[AGT]GC[ACT]^C
BpmI          750  CTCCAG               CTGGAG(16/14)
BsaHI         514  GGCGCC               G[GA]^CG[CT]C
BsaWI         229  TCCGGA               [AT]^CCGG[AT]
BseAI         229  TCCGGA               T^CCGGA
BsmAI         759  GTCTC                GTCTC(1/5)
BsmFI         260  GTCCC                GGGAC(10/14)
Bsp1286I      686  GGGCTC               G[AGT]GC[ACT]^C
BspEI         229  TCCGGA               T^CCGGA
BstUI           5  CGCG                 CG^CG
Cac8I         426  GCTGGC               GC[ACGT]^[ACGT]GC
DraIII        168  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          428  TGGCCA               [CT]^GGCC[GA]
EarI          325  GAAGAG               CTCTTC(1/4)
EcoT14I       287  CCAAGG               C^C[AT][AT]GG
EcoT22I       526  ATGCAT               ATGCA^T
HaeII         514  GGCGCC               [GA]GCGC^[CT]
HapII         230  CCGG                 C^CGG
Hin1I         514  GGCGCC               G[GA]^CG[CT]C
HindIII       346  AAGCTT               A^AGCTT
HpaII         230  CCGG                 C^CGG
KasI          514  GGCGCC               G^GCGCC
Ksp632I       325  GAAGAG               CTCTTC(1/4)
MaeI          464  CTAG                 C^TAG
MluNI         428  TGGCCA               TGG^CCA
MroI          229  TCCGGA               T^CCGGA
MscI          428  TGGCCA               TGG^CCA
MspI          230  CCGG                 C^CGG
MvnI            5  CGCG                 CG^CG
NarI          514  GGCGCC               GG^CGCC
NsiI          526  ATGCAT               ATGCA^T
PflMI         584  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          258  GAGTC                GAGTC(4/5)
PshAI         335  GACTACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          694  CTGCAG               CTGCA^G
SfoI          514  GGCGCC               GGC^GCC
StyI          287  CCAAGG               C^C[AT][AT]GG
TfiI          781  GAATC                G^A[AT]TC
Tsp45I        491  GTGAC                ^GT[GC]AC
Tsp509I       391  AATT                 ^AATT
Van91I        584  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          463  TCTAGA               T^CTAGA
XcmI          201  CCAGCTACCCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Asp700I        24  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              376  GAAAAGTTTC          
AvaII         291  GGACC                G^G[AT]CC
              536  GGACC               
BsgI          445  CTGCAC               GTGCAG(16/14)
              766  CTGCAC              
BsiYI         223  CCTCTTTCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              584  CCAGACCCTGG         
BslI          223  CCTCTTTCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              584  CCAGACCCTGG         
BstYI         137  AGATCC               [GA]^GATC[CT]
              563  AGATCT              
CfoI            6  GCGC                 GCG^C
              515  GCGC                
Cfr13I        291  GGACC                G^G[ACGT]CC
              536  GGACC               
DpnII         138  GATC                 ^GATC
              564  GATC                
HaeIII        214  GGCC                 GG^CC
              429  GGCC                
HhaI            6  GCGC                 GCG^C
              515  GCGC                
HinP1I          6  GCGC                 G^CGC
              515  GCGC                
HinfI         258  GAGTC                G^A[ACGT]TC
              781  GAATC               
MaeIII        107  GTTAC                ^GT[ACGT]AC
              491  GTGAC               
MboI          138  GATC                 ^GATC
              564  GATC                
MboII         325  GAAGA                GAAGA(8/7)
              522  GAAGA               
MflI          137  AGATCC               [GA]^GATC[CT]
              563  AGATCT              
NdeII         138  GATC                 ^GATC
              564  GATC                
NlaIV         514  GGCGCC               GG[ACGT]^[ACGT]CC
              536  GGACCC              
PvuII         442  CAGCTG               CAG^CTG
              477  CAGCTG              
Sau3AI        138  GATC                 ^GATC
              564  GATC                
Sau96I        291  GGACC                G^G[ACGT]CC
              536  GGACC               
SfaNI         454  GATGC                GCATC(5/9)
              525  GATGC               
XhoII         137  AGATCC               [GA]^GATC[CT]
              563  AGATCT              
XmnI           24  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              376  GAAAAGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BseRI       
BsrI        BssKI       BstAPI      BstNI       DdeI        
EcoRII      Fnu4HI      ItaI        MnlI        MslI        
MspA1I      MvaI        MwoI        NlaIII      ScrFI       
SfcI        TseI        TspRI       
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