Restriction map for pF1KB7545
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       CelII       ClaI        CpoI        DraI        
DraII       DraIII      EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MflI        
MluI        MroI        MunI        NarI        NciI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfcI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        TspRI       TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AlwI          198  GATCC                GGATC(4/5)
AocI          148  CCTGAGG              CC^T[ACGT]AGG
AseI          492  ATTAAT               AT^TAAT
AsnI          492  ATTAAT               AT^TAAT
AspHI         119  GAGCTC               G[AT]GC[AT]^C
AspI          529  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         283  GGTCC                G^G[AT]CC
BalI          111  TGGCCA               TGG^CCA
BanII         119  GAGCTC               G[GA]GC[CT]^C
BbsI          430  GAAGAC               GAAGAC(2/6)
BbvCI          73  CCTCAGC              CCTCAGC(-5/-2)
BpmI          122  CTCCAG               CTGGAG(16/14)
BpuAI         430  GAAGAC               GAAGAC(2/6)
BsaAI         512  TACGTG               [CT]AC^GT[GA]
BsiHKAI       119  GAGCTC               G[AT]GC[AT]^C
BspMI         339  GCAGGT               ACCTGC(4/8)
BsrFI         261  GCCGGC               [GA]^CCGG[CT]
BssSI         166  CACGAG               CACGAG(-5/-1)
Bst1107I       46  GTATAC               GTA^TAC
BstZ17I        46  GTATAC               GTA^TAC
Bsu36I        148  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        261  GCCGGC               [GA]^CCGG[CT]
DpnII         198  GATC                 ^GATC
DrdI          526  GACGACTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          275  CCATGG               C^C[GA][CT]GG
Eco81I        148  CCTGAGG              CC^T[ACGT]AGG
MaeII         513  ACGT                 A^CGT
MaeIII        424  GTGAC                ^GT[ACGT]AC
MboI          198  GATC                 ^GATC
MboII         430  GAAGA                GAAGA(8/7)
MluNI         111  TGGCCA               TGG^CCA
MscI          111  TGGCCA               TGG^CCA
MslI          540  CATCGTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         76  CAGCTG               C[AC]G^C[GT]G
NaeI          261  GCCGGC               GCC^GGC
NcoI          275  CCATGG               C^CATGG
NdeII         198  GATC                 ^GATC
NgoMIV        261  GCCGGC               G^CCGGC
NspI          299  ACATGC               [GA]CATG^[CT]
PflFI         529  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         492  ATTAAT               AT^TAAT
PvuII          76  CAGCTG               CAG^CTG
SacI          119  GAGCTC               GAGCT^C
Sau3AI        198  GATC                 ^GATC
SexAI          50  ACCAGGT              A^CC[AT]GGT
SfaNI          88  GCATC                GCATC(5/9)
StuI          126  AGGCCT               AGG^CCT
Tsp45I        424  GTGAC                ^GT[GC]AC
Tsp509I       491  AATT                 ^AATT
Tth111I       529  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           68  CCACACCTCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI           46  GTATAC               GT^[AC][GT]AC
              535  GTCTAC              
AccII           5  CGCG                 CG^CG
              184  CGCG                
AvaI          202  CCCGAG               C^[CT]CG[GA]G
              292  CCCGAG              
BglI          104  GCCATGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              265  GCCCAGGCGGC         
BmyI          119  GAGCTC               G[AGT]GC[ACT]^C
              388  GTGCCC              
BseRI         256  GAGGAG               GAGGAG(10/8)
              346  GAGGAG              
BsoBI         202  CCCGAG               C^[CT]CG[GA]G
              292  CCCGAG              
Bsp1286I      119  GAGCTC               G[AGT]GC[ACT]^C
              388  GTGCCC              
BstUI           5  CGCG                 CG^CG
              184  CGCG                
Cac8I         261  GCCGGC               GC[ACGT]^[ACGT]GC
              498  GCTCGC              
EaeI          111  TGGCCA               [CT]^GGCC[GA]
              272  CGGCCA              
EcoT14I       114  CCAAGG               C^C[AT][AT]GG
              275  CCATGG              
FauI          249  CCCGC                CCCGC(4/6)
              441  GCGGG               
HapII         253  CCGG                 C^CGG
              262  CCGG                
HpaII         253  CCGG                 C^CGG
              262  CCGG                
MseI          452  TTAA                 T^TAA
              493  TTAA                
MspI          253  CCGG                 C^CGG
              262  CCGG                
MvnI            5  CGCG                 CG^CG
              184  CGCG                
SfiI          103  GGCCATGTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              264  GGCCCAGGCGGCC       
StyI          114  CCAAGG               C^C[AT][AT]GG
              275  CCATGG              
Tru9I         452  TTAA                 T^TAA
              493  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstNI       CfoI        Cfr13I      
DdeI        EcoRII      Fnu4HI      HaeIII      HhaI        
HinP1I      ItaI        MnlI        MvaI        MwoI        
NlaIII      NlaIV       Sau96I      ScrFI       TseI        
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