Restriction map for pF1KB7545
 (Linear) (Six-base) MAPSORT of: KIBB7545.seq  Check: 8682  from: 1  to: 552



KIBB7545.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     330     552 
   Size:        330     222
 
AccI GT'mk_AC

Cuts at:      0      47     536     552 
   Size:         47     489      16

  Fragments arranged by size: 

                 489      47      16
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     552 
   Size:        449     103
 
AseI AT'TA_AT

Cuts at:      0     493     552 
   Size:        493      59
 
AvaI C'yCGr_G

Cuts at:      0     202     292     552 
   Size:        202      90     260

  Fragments arranged by size: 

                 260     202      90
 
BanII G_rGCy'C

Cuts at:      0     123     552 
   Size:        123     429
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     437     552 
   Size:        437     115
 
BbvCI CC'TCA_GC

Cuts at:      0      74     552 
   Size:         74     478
 
BglI GCCn_nnn'nGGC

Cuts at:      0     110     271     552 
   Size:        110     161     281

  Fragments arranged by size: 

                 281     161     110
 
Bme1580I G_kGCm'C

Cuts at:      0     392     552 
   Size:        392     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     552 
   Size:        107     445
 
Bpu10I CC'TnA_GC

Cuts at:      0      74     552 
   Size:         74     478
 
BsaAI yAC'GTr

Cuts at:      0     514     552 
   Size:        514      38
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     361     552 
   Size:        271      90     191

  Fragments arranged by size: 

                 271     191      90
 
BseYI C'CCAG_C

Cuts at:      0      78     552 
   Size:         78     474
 
BsiHKAI G_wGCw'C

Cuts at:      0     123     552 
   Size:        123     429
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     392     552 
   Size:        123     269     160

  Fragments arranged by size: 

                 269     160     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     330     552 
   Size:        330     222
 
BsrFI r'CCGG_y

Cuts at:      0     261     552 
   Size:        261     291
 
BssSI C'ACGA_G

Cuts at:      0     166     552 
   Size:        166     386
 
BstZ17I GTA'TAC

Cuts at:      0      48     552 
   Size:         48     504
 
Bsu36I CC'TnA_GG

Cuts at:      0     149     552 
   Size:        149     403
 
BtgI C'CryG_G

Cuts at:      0     275     552 
   Size:        275     277
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     200     552 
   Size:        200     352
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     532     552 
   Size:        532      20
 
EaeI y'GGCC_r

Cuts at:      0     111     272     552 
   Size:        111     161     280

  Fragments arranged by size: 

                 280     161     111
 
EcoICRI GAG'CTC

Cuts at:      0     121     552 
   Size:        121     431
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     449     552 
   Size:        107     342     103

  Fragments arranged by size: 

                 342     107     103
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     552 
   Size:        390     162
 
MscI TGG'CCA

Cuts at:      0     113     552 
   Size:        113     439
 
MslI CAynn'nnrTG

Cuts at:      0     544     552 
   Size:        544       8
 
MspA1I CmG'CkG

Cuts at:      0      78     552 
   Size:         78     474
 
NaeI GCC'GGC

Cuts at:      0     263     552 
   Size:        263     289
 
NcoI C'CATG_G

Cuts at:      0     275     552 
   Size:        275     277
 
NgoMIV G'CCGG_C

Cuts at:      0     261     552 
   Size:        261     291
 
NspI r_CATG'y

Cuts at:      0     303     552 
   Size:        303     249
 
PvuII CAG'CTG

Cuts at:      0      78     552 
   Size:         78     474
 
SacI G_AGCT'C

Cuts at:      0     123     552 
   Size:        123     429
 
SexAI A'CCwGG_T

Cuts at:      0      50     552 
   Size:         50     502
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     110     271     552 
   Size:        110     161     281

  Fragments arranged by size: 

                 281     161     110
 
StuI AGG'CCT

Cuts at:      0     128     552 
   Size:        128     424
 
StyI C'CwwG_G

Cuts at:      0     114     275     552 
   Size:        114     161     277

  Fragments arranged by size: 

                 277     161     114
 
Tth111I GACn'n_nGTC

Cuts at:      0     532     552 
   Size:        532      20
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      75     552 
   Size:         75     477
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AseI     AvaI    BanII     BbsI    BbvCI
     BglI Bme1580I     BpmI   Bpu10I    BsaAI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrFI    BssSI  BstZ17I   Bsu36I     BtgI    BtgZI
     DrdI     EaeI  EcoICRI  Eco57MI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI    PvuII     SacI    SexAI     SfiI
     StuI     StyI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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