Restriction map for pF1KB7541
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaI        BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HindIII     HphI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII          21  CGCG                 CG^CG
AfaI          135  GTAC                 GT^AC
AgeI          218  ACCGGT               A^CCGGT
AlwNI         157  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          162  CCTGAGG              CC^T[ACGT]AGG
BamHI         318  GGATCC               G^GATCC
BanI           33  GGCGCC               G^G[CT][GA]CC
BbeI           33  GGCGCC               GGCGC^C
BfaI          404  CTAG                 C^TAG
BglI          514  GCCATGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         149  AGATCT               A^GATCT
BlpI           61  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       61  GCTCAGC              GC^T[ACGT]AGC
BsaWI         218  ACCGGT               [AT]^CCGG[AT]
BsgI          121  GTGCAG               GTGCAG(16/14)
BsmAI         115  GAGAC                GTCTC(1/5)
BsrFI         218  ACCGGT               [GA]^CCGG[CT]
BstNI          57  CCAGG                CC^[AT]GG
BstUI          21  CGCG                 CG^CG
Bsu36I        162  CCTGAGG              CC^T[ACGT]AGG
CelII          61  GCTCAGC              GC^T[ACGT]AGC
CfoI           34  GCGC                 GCG^C
Cfr10I        218  ACCGGT               [GA]^CCGG[CT]
DsaI          143  CCATGG               C^C[GA][CT]GG
EaeI           17  TGGCCG               [CT]^GGCC[GA]
Eco81I        162  CCTGAGG              CC^T[ACGT]AGG
EcoRII         57  CCAGG                ^CC[AT]GG
EcoT14I       143  CCATGG               C^C[AT][AT]GG
HaeII          33  GGCGCC               [GA]GCGC^[CT]
HhaI           34  GCGC                 GCG^C
HinP1I         34  GCGC                 G^CGC
HinfI         456  GAATC                G^A[ACGT]TC
HpaI          222  GTTAAC               GTT^AAC
KasI           33  GGCGCC               G^GCGCC
MaeI          404  CTAG                 C^TAG
MaeIII        190  GTCAC                ^GT[ACGT]AC
MseI          223  TTAA                 T^TAA
MvaI           57  CCAGG                CC^[AT]GG
MvnI           21  CGCG                 CG^CG
NarI           33  GGCGCC               GG^CGCC
NcoI          143  CCATGG               C^CATGG
NdeI          347  CATATG               CA^TATG
NspI          170  ACATGC               [GA]CATG^[CT]
PinAI         218  ACCGGT               A^CCGGT
RsaI          135  GTAC                 GT^AC
SfaNI         104  GCATC                GCATC(5/9)
SfcI          232  CTACAG               C^T[GA][CT]AG
SfoI           33  GGCGCC               GGC^GCC
StyI          143  CCATGG               C^C[AT][AT]GG
TaqI          332  TCGA                 T^CGA
TfiI          456  GAATC                G^A[AT]TC
Tru9I         223  TTAA                 T^TAA
Tsp45I        190  GTCAC                ^GT[GC]AC
TthHB8I       332  TCGA                 T^CGA
XcmI            5  CCACCCCTCCGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI           33  GGCGCC               G[GA]^CG[CT]C
               90  GGCGTC              
ApaI           40  GGGCCC               GGGCC^C
               52  GGGCCC              
AvaI           37  CCCGGG               C^[CT]CG[GA]G
               74  CCCGGG              
BanII          40  GGGCCC               G[GA]GC[CT]^C
               52  GGGCCC              
BbvI          174  GCAGC                GCAGC(8/12)
              177  GCTGC               
BmyI           40  GGGCCC               G[AGT]GC[ACT]^C
               52  GGGCCC              
BsaHI          33  GGCGCC               G[GA]^CG[CT]C
               90  GGCGTC              
BsoBI          37  CCCGGG               C^[CT]CG[GA]G
               74  CCCGGG              
Bsp1286I       40  GGGCCC               G[AGT]GC[ACT]^C
               52  GGGCCC              
BsrDI         296  GCAATG               GCAATG(2/0)
              523  GCAATG              
BsrI          202  ACTGG                ACTGG(1/-1)
              328  CCAGT               
BstF5I         29  GGATG                GGATG(2/0)
              314  GGATG               
BstYI         149  AGATCT               [GA]^GATC[CT]
              318  GGATCC              
Cac8I          66  GCAGGC               GC[ACGT]^[ACGT]GC
              385  GCTGGC              
DraII          40  GGGCCCT              [GA]G^G[ACGT]CC[CT]
               52  GGGCCCC             
EarI          262  CTCTTC               CTCTTC(1/4)
              409  GAAGAG              
EcoO109I       40  GGGCCCT              [GA]G^G[ACGT]CC[CT]
               52  GGGCCCC             
FokI           29  GGATG                GGATG(9/13)
              314  GGATG               
HgaI           22  GCGTC                GACGC(5/10)
               91  GCGTC               
Hin1I          33  GGCGCC               G[GA]^CG[CT]C
               90  GGCGTC              
HincII         82  GTCAAC               GT[CT]^[GA]AC
              222  GTTAAC              
HindII         82  GTCAAC               GT[CT]^[GA]AC
              222  GTTAAC              
Ksp632I       262  CTCTTC               CTCTTC(1/4)
              409  GAAGAG              
MflI          149  AGATCT               [GA]^GATC[CT]
              318  GGATCC              
MspA1I        175  CAGCTG               C[AC]G^C[GT]G
              463  CAGCTG              
MwoI           61  GCTCAGCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              514  GCCATGTTGGC         
PvuII         175  CAGCTG               CAG^CTG
              463  CAGCTG              
SmaI           37  CCCGGG               CCC^GGG
               74  CCCGGG              
TseI          174  GCAGC                G^C[AT]GC
              177  GCTGC               
TspRI         119  CAGTG                CA[GC]TG[ACGT][ACGT]^
              201  CACTG               
XhoII         149  AGATCT               [GA]^GATC[CT]
              318  GGATCC              
XmaCI          37  CCCGGG               C^CCGGG
               74  CCCGGG              
XmaI           37  CCCGGG               C^CCGGG
               74  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AluI        AlwI        BcnI        BsaJI       
BsiYI       BslI        BssKI       Cfr13I      DdeI        
DpnII       Fnu4HI      HaeIII      HapII       HpaII       
ItaI        MboI        MboII       MnlI        MspI        
NciI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       Tsp509I     
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