Restriction map for pF1KB7538
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       CfoI        Cfr10I      
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MspA1I      MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI           69  GAATTC               [GA]^AATT[CT]
AlwI          658  GGATC                GGATC(4/5)
AocI          541  CCTCAGG              CC^T[ACGT]AGG
ApoI           69  GAATTC               [GA]^AATT[CT]
Asp700I       336  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         140  GAGCTC               G[AT]GC[AT]^C
AvaI          180  CTCGAG               C^[CT]CG[GA]G
AvaII         322  GGTCC                G^G[AT]CC
BalI          576  TGGCCA               TGG^CCA
BbsI           15  GAAGAC               GAAGAC(2/6)
BpuAI          15  GAAGAC               GAAGAC(2/6)
BsiHKAI       140  GAGCTC               G[AT]GC[AT]^C
BsmBI         248  CGTCTC               CGTCTC(1/5)
BsoBI         180  CTCGAG               C^[CT]CG[GA]G
BspDI         755  ATCGAT               AT^CGAT
BsrBI         452  CCGCTC               CCGCTC(-3/-3)
BssSI         653  CTCGTG               CACGAG(-5/-1)
BstYI         658  GGATCT               [GA]^GATC[CT]
Bsu36I        541  CCTCAGG              CC^T[ACGT]AGG
Cac8I         409  GCGGGC               GC[ACGT]^[ACGT]GC
ClaI          755  ATCGAT               AT^CGAT
DpnII         659  GATC                 ^GATC
DraII         364  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          576  TGGCCA               [CT]^GGCC[GA]
EarI          604  CTCTTC               CTCTTC(1/4)
Eco81I        541  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      364  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI          69  GAATTC               G^AATTC
Ksp632I       604  CTCTTC               CTCTTC(1/4)
MaeII         492  ACGT                 A^CGT
MboI          659  GATC                 ^GATC
MflI          658  GGATCT               [GA]^GATC[CT]
MluNI         576  TGGCCA               TGG^CCA
MscI          576  TGGCCA               TGG^CCA
MslI          327  CATGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         659  GATC                 ^GATC
PaeR7I        180  CTCGAG               C^TCGAG
PleI          183  GAGTC                GAGTC(4/5)
PstI          763  CTGCAG               CTGCA^G
SacI          140  GAGCTC               GAGCT^C
SapI          603  GCTCTTC              GCTCTTC(1/4)
Sau3AI        659  GATC                 ^GATC
SexAI         461  ACCTGGT              A^CC[AT]GGT
SfcI          763  CTGCAG               C^T[GA][CT]AG
SmlI          180  CTCGAG               C^T[CT][GA]AG
Tsp45I        283  GTCAC                ^GT[GC]AC
XcmI          730  CCACCAAACCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          180  CTCGAG               C^TCGAG
XhoII         658  GGATCT               [GA]^GATC[CT]
XmnI          336  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AfaI          277  GTAC                 GT^AC
              668  GTAC                
BanI          412  GGCACC               G^G[CT][GA]CC
              458  GGCACC              
BcnI           74  CCCGG                CC^[GC]GG
              619  CCCGG               
BfaI          359  CTAG                 C^TAG
              532  CTAG                
BpmI          135  CTCCAG               CTGGAG(16/14)
              514  CTCCAG              
BseRI         399  CTCCTC               GAGGAG(10/8)
              511  CTCCTC              
BspMI         611  ACCTGC               ACCTGC(4/8)
              761  ACCTGC              
BsrI          740  ACTGG                ACTGG(1/-1)
              665  CCAGT               
DsaI          253  CCATGG               C^C[GA][CT]GG
              326  CCATGG              
FauI          451  CCCGC                CCCGC(4/6)
              409  GCGGG               
HphI           89  TCACC                GGTGA(8/7)
              346  TCACC               
MaeI          359  CTAG                 C^TAG
              532  CTAG                
MaeIII        242  GTTAC                ^GT[ACGT]AC
              283  GTCAC               
NciI           74  CCCGG                CC^[GC]GG
              619  CCCGG               
NcoI          253  CCATGG               C^CATGG
              326  CCATGG              
NspI          221  GCATGT               [GA]CATG^[CT]
              312  GCATGT              
RsaI          277  GTAC                 GT^AC
              668  GTAC                
TaqI          181  TCGA                 T^CGA
              756  TCGA                
TfiI          336  GAATC                G^A[AT]TC
              547  GAATC               
Tsp509I        70  AATT                 ^AATT
              170  AATT                
TspRI         235  CAGTG                CA[GC]TG[ACGT][ACGT]^
              739  CACTG               
TthHB8I       181  TCGA                 T^CGA
              756  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       BstXI       
Cfr13I      DdeI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
ItaI        MboII       MnlI        MspI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       StyI        TseI        
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