Restriction map for pF1KB7535
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          44  CGCG                 CG^CG
AfaI          213  GTAC                 GT^AC
AluI           86  AGCT                 AG^CT
ApaI          304  GGGCCC               GGGCC^C
Asp700I        84  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          22  GAGCAC               G[AT]GC[AT]^C
BanII         304  GGGCCC               G[GA]GC[CT]^C
BbvI           36  GCTGC                GCAGC(8/12)
BfaI           19  CTAG                 C^TAG
BsiHKAI        22  GAGCAC               G[AT]GC[AT]^C
BspMI         109  ACCTGC               ACCTGC(4/8)
BsrI          205  CCAGT                ACTGG(1/-1)
BssKI         181  CCAGG                ^CC[ACGT]GG
BstNI         181  CCAGG                CC^[AT]GG
BstUI          44  CGCG                 CG^CG
BstYI         425  AGATCC               [GA]^GATC[CT]
CfoI           45  GCGC                 GCG^C
DdeI          269  CTCAG                C^T[ACGT]AG
DraI          238  TTTAAA               TTT^AAA
EaeI          232  CGGCCA               [CT]^GGCC[GA]
EcoRII        181  CCAGG                ^CC[AT]GG
FauI           42  CCCGC                CCCGC(4/6)
Fnu4HI         36  GCTGC                GC^[ACGT]GC
HapII         231  CCGG                 C^CGG
HhaI           45  GCGC                 GCG^C
HinP1I         45  GCGC                 G^CGC
HincII        274  GTCAAC               GT[CT]^[GA]AC
HindII        274  GTCAAC               GT[CT]^[GA]AC
HpaII         231  CCGG                 C^CGG
ItaI           36  GCTGC                GC^[ACGT]GC
MaeI           19  CTAG                 C^TAG
MflI          425  AGATCC               [GA]^GATC[CT]
MslI           93  CACCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          231  CCGG                 C^CGG
MvaI          181  CCAGG                CC^[AT]GG
MvnI           44  CGCG                 CG^CG
PleI          287  GACTC                GAGTC(4/5)
RsaI          213  GTAC                 GT^AC
SapI           87  GCTCTTC              GCTCTTC(1/4)
ScrFI         181  CCAGG                CC^[ACGT]GG
SfaNI         256  GATGC                GCATC(5/9)
SspI          157  AATATT               AAT^ATT
TaqI          327  TCGA                 T^CGA
TfiI          408  GATTC                G^A[AT]TC
TseI           36  GCTGC                G^C[AT]GC
TthHB8I       327  TCGA                 T^CGA
XhoII         425  AGATCC               [GA]^GATC[CT]
XmnI           84  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI           43  CCGC                 CCGC(-3/-1)
              429  CCGC                
AvaII         107  GGACC                G^G[AT]CC
              446  GGACC               
BmyI           22  GAGCAC               G[AGT]GC[ACT]^C
              304  GGGCCC              
BsaJI          95  CCGAGG               C^C[ACGT][ACGT]GG
              186  CCGAGG              
BsgI           37  CTGCAC               GTGCAG(16/14)
              111  CTGCAC              
BsmFI         106  GGGAC                GGGAC(10/14)
              445  GGGAC               
Bsp1286I       22  GAGCAC               G[AGT]GC[ACT]^C
              304  GGGCCC              
DraII         106  GGGACCT              [GA]G^G[ACGT]CC[CT]
              445  GGGACCC             
EarI           88  CTCTTC               CTCTTC(1/4)
              289  CTCTTC              
EcoO109I      106  GGGACCT              [GA]G^G[ACGT]CC[CT]
              445  GGGACCC             
HinfI         287  GACTC                G^A[ACGT]TC
              408  GATTC               
Ksp632I        88  CTCTTC               CTCTTC(1/4)
              289  CTCTTC              
MboII          89  TCTTC                GAAGA(8/7)
              290  TCTTC               
MwoI           36  GCTGCACCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               47  GCTACTTCGGC         
NlaIII        309  CATG                 CATG^
              377  CATG                
PpuMI         106  GGGACCT              [GA]G^G[AT]CC[CT]
              445  GGGACCC             
Tsp45I        142  GTCAC                ^GT[GC]AC
              265  GTCAC               
Tsp509I       135  AATT                 ^AATT
              300  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AlwI        BsiYI       BslI        BstF5I      Cfr13I      
DpnII       FokI        HaeIII      HphI        MaeIII      
MboI        MnlI        MseI        NdeII       NlaIV       
Sau3AI      Sau96I      Tru9I       
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