Restriction map for
pF1KB7516
(Circular) (Six-base) MAPSORT of: pF1KB7516.seq Check: 8426 from: 1 to: 3851
pF1KB7516 3851 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 189 889 189
Size: 700 3151
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 596 3691 596
Size: 3095 756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 673 1676 2108 2855 3123 189
Size: 484 1003 432 747 268 917
Fragments arranged by size:
1003 917 747 484 432 268
AflIII A'CryG_T
Cuts at: 1203 2307 3284 1203
Size: 1104 977 1770
Fragments arranged by size:
1770 1104 977
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 1156 2723 1156
Size: 1567 2284
ApaLI G'TGCA_C
Cuts at: 171 2621 171
Size: 2450 1401
ApoI r'AATT_y
Cuts at: 855 3204 3460 855
Size: 2349 256 1246
Fragments arranged by size:
2349 1246 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 871 871
Size: 3851
BamHI G'GATC_C
Cuts at: 876 876
Size: 3851
BanI G'GyrC_C
Cuts at: 867 1281 1529 1564 867
Size: 414 248 35 3154
Fragments arranged by size:
3154 414 248 35
BanII G_rGCy'C
Cuts at: 865 1895 865
Size: 1030 2821
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BbsI GAAGACnn'nnnn_
Cuts at: 661 661
Size: 3851
BbvCI CC'TCA_GC
Cuts at: 836 836
Size: 3851
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 1371 1371
Size: 3851
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 147 175 1476 1569 2625 147
Size: 28 1301 93 1056 1373
Fragments arranged by size:
1373 1301 1056 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 3851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 179
Size: 3851
Bpu10I CC'TnA_GC
Cuts at: 626 836 626
Size: 210 3641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2398 2696 1027
Size: 879 492 298 2182
Fragments arranged by size:
2182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 1282 1530 2232 3728 1282
Size: 248 702 1496 1405
Fragments arranged by size:
1496 1405 702 248
BsaWI w'CCGG_w
Cuts at: 1040 1561 2239 2513 2660 3071 1040
Size: 521 678 274 147 411 1820
Fragments arranged by size:
1820 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 853 853
Size: 3851
BseYI C'CCAG_C
Cuts at: 362 1276 2034 2611 3107 362
Size: 914 758 577 496 1106
Fragments arranged by size:
1106 914 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 175 865 1643 1833 2625 175
Size: 690 778 190 792 1401
Fragments arranged by size:
1401 792 778 690 190
Bsp1286I G_dGCh'C
Cuts at: 147 175 865 1476 1569 1643 1833 1895 2625
Size: 28 690 611 93 74 190 62 730
Cuts at: 2625 147
Size: 1373
Fragments arranged by size:
1373 730 690 611 190 93 74 62
28
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 420 901 1115 1417 1798 420
Size: 481 214 302 381 2473
Fragments arranged by size:
2473 481 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1763 1763
Size: 3851
BsrFI r'CCGG_y
Cuts at: 175 1849 2030 2239 175
Size: 1674 181 209 1787
Fragments arranged by size:
1787 1674 209 181
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 1228
Size: 3851
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstYI r'GATC_y
Cuts at: 1 792 876 1343 1366 1701 1947 2959 3045
Size: 791 84 467 23 335 246 1012 86
Cuts at: 3045 3057 3403 1
Size: 12 346 449
Fragments arranged by size:
1012 791 467 449 346 335 246 86
84 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3851
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 556 1094 556
Size: 538 3313
DraI TTT'AAA
Cuts at: 312 459 521 850 312
Size: 147 62 329 3313
Fragments arranged by size:
3313 329 147 62
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2415 1557
Size: 858 2993
EaeI y'GGCC_r
Cuts at: 1436 1610 2001 2028 3373 1436
Size: 174 391 27 1345 1914
Fragments arranged by size:
1914 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 437 1874 2084 437
Size: 1437 210 2204
Fragments arranged by size:
2204 1437 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 436 2059 2369 2515 3661 436
Size: 1623 310 146 1146 626
Fragments arranged by size:
1623 1146 626 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 189 673 1676 2108 2855 3123 179
Size: 10 484 1003 432 747 268 907
Fragments arranged by size:
1003 907 747 484 432 268 10
EcoO109I rG'GnC_Cy
Cuts at: 990 990
Size: 3851
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3851
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 1285 1533 2555 3534 1285
Size: 248 1022 979 1602
Fragments arranged by size:
1602 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1693 1723 1725 1755 1693
Size: 30 2 30 3789
Fragments arranged by size:
3789 30 30 2
HincII GTy'rAC
Cuts at: 190 554 890 190
Size: 364 336 3151
Fragments arranged by size:
3151 364 336
HpaI GTT'AAC
Cuts at: 554 554
Size: 3851
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 349 762 2522 2706 349
Size: 413 1760 184 1494
Fragments arranged by size:
1760 1494 413 184
MscI TGG'CCA
Cuts at: 1612 1612
Size: 3851
MslI CAynn'nnrTG
Cuts at: 1840 1967 1840
Size: 127 3724
MspA1I CmG'CkG
Cuts at: 962 1276 1636 2649 2894 962
Size: 314 360 1013 245 1919
Fragments arranged by size:
1919 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NcoI C'CATG_G
Cuts at: 107 107
Size: 3851
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 129 718 904 1404 2311 129
Size: 589 186 500 907 1669
Fragments arranged by size:
1669 907 589 500 186
PasI CC'CwG_GG
Cuts at: 147 147
Size: 3851
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PsiI TTA'TAA
Cuts at: 593 593
Size: 3851
PstI C_TGCA'G
Cuts at: 898 1583 898
Size: 685 3166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 1276 1636 1276
Size: 360 3491
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 188 888 188
Size: 700 3151
SapI GCTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SfcI C'TryA_G
Cuts at: 33 338 894 1579 2572 2763 33
Size: 305 556 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 305 191
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 873 873
Size: 3851
SmlI C'TyrA_G
Cuts at: 1006 1921 2413 2675 1006
Size: 915 492 262 2182
Fragments arranged by size:
2182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 718 904 1404 718
Size: 186 500 3165
Fragments arranged by size:
3165 500 186
StyI C'CwwG_G
Cuts at: 107 985 1339 107
Size: 878 354 2619
Fragments arranged by size:
2619 878 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1434 1567 2026 2235 1434
Size: 133 459 209 3050
Fragments arranged by size:
3050 459 209 133
TatI w'GTAC_w
Cuts at: 3366 3366
Size: 3851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 3819
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1346 1346
Size: 3851
XmaI C'CCGG_G
Cuts at: 871 871
Size: 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI