Restriction map for pF1KB7515
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraII       DraIII      DsaI        Eam1105I    EarI        
Eco47III    EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          48  CGCG                 CG^CG
AcyI           51  GGCGCC               G[GA]^CG[CT]C
AocI           94  CCTGAGG              CC^T[ACGT]AGG
AspHI         289  GAGCAC               G[AT]GC[AT]^C
AvaI           55  CCCGGG               C^[CT]CG[GA]G
BanI           51  GGCGCC               G^G[CT][GA]CC
BbeI           51  GGCGCC               GGCGC^C
BglI          342  GCCTCCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          208  CTGGAG               CTGGAG(16/14)
BsaHI          51  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       289  GAGCAC               G[AT]GC[AT]^C
BsoBI          55  CCCGGG               C^[CT]CG[GA]G
BsrI          337  CCAGT                ACTGG(1/-1)
BssSI         292  CACGAG               CACGAG(-5/-1)
BstF5I         99  GGATG                GGATG(2/0)
BstNI         207  CCTGG                CC^[AT]GG
BstUI          48  CGCG                 CG^CG
Bsu36I         94  CCTGAGG              CC^T[ACGT]AGG
CfoI           52  GCGC                 GCG^C
DpnII         240  GATC                 ^GATC
DrdI          106  GACAGGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         94  CCTGAGG              CC^T[ACGT]AGG
EcoNI         270  CCTGACAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        207  CCTGG                ^CC[AT]GG
FauI          306  CCCGC                CCCGC(4/6)
FokI           99  GGATG                GGATG(9/13)
HaeII          51  GGCGCC               [GA]GCGC^[CT]
HhaI           52  GCGC                 GCG^C
Hin1I          51  GGCGCC               G[GA]^CG[CT]C
HinP1I         52  GCGC                 G^CGC
KasI           51  GGCGCC               G^GCGCC
MboI          240  GATC                 ^GATC
MspA1I        307  CCGCTG               C[AC]G^C[GT]G
MvaI          207  CCTGG                CC^[AT]GG
MvnI           48  CGCG                 CG^CG
NarI           51  GGCGCC               GG^CGCC
NdeII         240  GATC                 ^GATC
PstI           34  CTGCAG               CTGCA^G
Sau3AI        240  GATC                 ^GATC
SfcI           34  CTGCAG               C^T[GA][CT]AG
SfoI           51  GGCGCC               GGC^GCC
SmaI           55  CCCGGG               CCC^GGG
XmaCI          55  CCCGGG               C^CCGGG
XmaI           55  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaII         114  GGTCC                G^G[AT]CC
              354  GGACC               
BanII          10  GGGCTC               G[GA]GC[CT]^C
               90  GAGCCC              
BsaJI          55  CCCGGG               C^C[ACGT][ACGT]GG
              346  CCCCGG              
BseRI          39  GAGGAG               GAGGAG(10/8)
              256  GAGGAG              
BsiEI          17  CGGCCG               CG[GA][CT]^CG
              349  CGGCCG              
BsrFI          22  GCCGGC               [GA]^CCGG[CT]
              364  GCCGGC              
Cfr10I         22  GCCGGC               [GA]^CCGG[CT]
              364  GCCGGC              
Cfr13I        114  GGTCC                G^G[ACGT]CC
              354  GGACC               
DdeI           95  CTGAG                C^T[ACGT]AG
              146  CTCAG               
EagI           17  CGGCCG               C^GGCCG
              349  CGGCCG              
EclXI          17  CGGCCG               C^GGCCG
              349  CGGCCG              
Eco52I         17  CGGCCG               C^GGCCG
              349  CGGCCG              
HgaI           29  GCGTC                GACGC(5/10)
               44  GCGTC               
MboII         159  GAAGA                GAAGA(8/7)
              297  GAAGA               
NaeI           22  GCCGGC               GCC^GGC
              364  GCCGGC              
NgoMIV         22  GCCGGC               G^CCGGC
              364  GCCGGC              
NlaIII        190  CATG                 CATG^
              225  CATG                
Sau96I        114  GGTCC                G^G[ACGT]CC
              354  GGACC               
TspRI         281  CACTG                CA[GC]TG[ACGT][ACGT]^
              338  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BmyI        
BsiYI       BslI        Bsp1286I    BssKI       Cac8I       
EaeI        Fnu4HI      HaeIII      HapII       HpaII       
ItaI        MnlI        MspI        MwoI        NciI        
NlaIV       ScrFI       TseI        
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