Restriction map for pF1KB7512
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MfeI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
XbaI        XcmI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AlwI          121  GATCC                GGATC(4/5)
AspHI          91  GAGCAC               G[AT]GC[AT]^C
AspI          258  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          278  CTCGAG               C^[CT]CG[GA]G
AvaII         443  GGACC                G^G[AT]CC
BanI          668  GGCACC               G^G[CT][GA]CC
BbvCI         477  CCTCAGC              CCTCAGC(-5/-2)
BcnI          693  CCCGG                CC^[GC]GG
BmyI           91  GAGCAC               G[AGT]GC[ACT]^C
BsaBI         115  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI        91  GAGCAC               G[AT]GC[AT]^C
BsmAI         276  GTCTC                GTCTC(1/5)
BsmFI         222  GTCCC                GGGAC(10/14)
BsoBI         278  CTCGAG               C^[CT]CG[GA]G
Bsp1286I       91  GAGCAC               G[AGT]GC[ACT]^C
BspDI         112  ATCGAT               AT^CGAT
BspHI         191  TCATGA               T^CATGA
BspMI          37  ACCTGC               ACCTGC(4/8)
BsrI          526  CCAGT                ACTGG(1/-1)
BstXI         306  CCACAGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         120  AGATCC               [GA]^GATC[CT]
ClaI          112  ATCGAT               AT^CGAT
DpnII         121  GATC                 ^GATC
DraII         659  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          124  CCACGG               C^C[GA][CT]GG
EaeI           77  TGGCCG               [CT]^GGCC[GA]
EcoO109I      659  AGGGCCT              [GA]G^G[ACGT]CC[CT]
FauI          206  CCCGC                CCCGC(4/6)
HapII         694  CCGG                 C^CGG
HpaII         694  CCGG                 C^CGG
HphI          347  TCACC                GGTGA(8/7)
MamI          115  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          121  GATC                 ^GATC
MflI          120  AGATCC               [GA]^GATC[CT]
MspI          694  CCGG                 C^CGG
NciI          693  CCCGG                CC^[GC]GG
NdeII         121  GATC                 ^GATC
NlaIV         668  GGCACC               GG[ACGT]^[ACGT]CC
PaeR7I        278  CTCGAG               C^TCGAG
PflFI         258  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         526  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          191  TCATGA               T^CATGA
Sau3AI        121  GATC                 ^GATC
SmlI          278  CTCGAG               C^T[CT][GA]AG
Tth111I       258  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        526  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          278  CTCGAG               C^TCGAG
XhoII         120  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

Cfr13I        443  GGACC                G^G[ACGT]CC
              660  GGGCC               
HaeIII         78  GGCC                 GG^CC
              661  GGCC                
MwoI          311  GCAGTGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              482  GCTCCATGTGC         
Sau96I        443  GGACC                G^G[ACGT]CC
              660  GGGCC               
Tsp45I        334  GTGAC                ^GT[GC]AC
              590  GTCAC               
Tsp509I       146  AATT                 ^AATT
              682  AATT                
TspRI         272  CAGTG                CA[GC]TG[ACGT][ACGT]^
              312  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AluI        AlwNI       BbvI        BsaJI       
BseRI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       DdeI        EarI        EcoRII      
Fnu4HI      FokI        HinfI       ItaI        Ksp632I     
MaeIII      MboII       MnlI        MslI        MvaI        
NlaIII      PleI        ScrFI       SfaNI       TaqI        
TfiI        TseI        TthHB8I     
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