Restriction map for pF1KB7451
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BbrPI       BbvCI       BciVI       
BclI        BcnI        BfaI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NciI        NdeI        NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          978  AAATTC               [GA]^AATT[CT]
AflII        1048  CTTAAG               C^TTAAG
ApoI          978  AAATTC               [GA]^AATT[CT]
AvaI          540  CTCGGG               C^[CT]CG[GA]G
BbeI            9  GGCGCC               GGCGC^C
BbsI          153  GTCTTC               GAAGAC(2/6)
BcgI          285  GCAGTGCAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1048  CTTAAG               C^TTAAG
BlpI          243  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      243  GCTCAGC              GC^T[ACGT]AGC
BpuAI         153  GTCTTC               GAAGAC(2/6)
BsiEI         197  CGGCCG               CG[GA][CT]^CG
BsmBI         999  GAGACG               CGTCTC(1/5)
BsoBI         540  CTCGGG               C^[CT]CG[GA]G
BspMI         533  ACCTGC               ACCTGC(4/8)
BsrBI        1167  CCGCTC               CCGCTC(-3/-3)
BssSI          14  CACGAG               CACGAG(-5/-1)
BstAPI        465  GCAGTATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         243  GCTCAGC              GC^T[ACGT]AGC
DraII        1104  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          669  CCATGG               C^C[GA][CT]GG
EagI          197  CGGCCG               C^GGCCG
EclXI         197  CGGCCG               C^GGCCG
Eco52I        197  CGGCCG               C^GGCCG
EcoNI         497  CCTTTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1104  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HincII        612  GTCAAC               GT[CT]^[GA]AC
HindII        612  GTCAAC               GT[CT]^[GA]AC
HindIII       546  AAGCTT               A^AGCTT
KasI            9  GGCGCC               G^GCGCC
NaeI          202  GCCGGC               GCC^GGC
NarI            9  GGCGCC               GG^CGCC
NcoI          669  CCATGG               C^CATGG
NgoMIV        202  GCCGGC               G^CCGGC
NotI          196  GCGGCCGC             GC^GGCCGC
NspI         1141  ACATGC               [GA]CATG^[CT]
PflMI         744  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1104  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          859  CTGCAG               CTGCA^G
SapI          787  GAAGAGC              GCTCTTC(1/4)
ScaI         1052  AGTACT               AGT^ACT
SfcI          859  CTGCAG               C^T[GA][CT]AG
SfoI            9  GGCGCC               GGC^GCC
SgrAI         207  CACCGGCG             C[GA]^CCGG[CT]G
TfiI          664  GAATC                G^A[AT]TC
Van91I        744  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          451  GTAGAC               GT^[AC][GT]AC
              943  GTCTAC              
AccII         129  CGCG                 CG^CG
              189  CGCG                
AcyI            9  GGCGCC               G[GA]^CG[CT]C
             1115  GACGCC              
BalI          585  TGGCCA               TGG^CCA
              672  TGGCCA              
BamHI         603  GGATCC               G^GATCC
             1125  GGATCC              
BanI            9  GGCGCC               G^G[CT][GA]CC
              205  GGCACC              
BpmI          238  CTGGAG               CTGGAG(16/14)
              751  CTGGAG              
BsaHI           9  GGCGCC               G[GA]^CG[CT]C
             1115  GACGCC              
BsmAI         999  GAGAC                GTCTC(1/5)
             1099  GAGAC               
BsmFI         132  GTCCC                GGGAC(10/14)
             1106  GTCCC               
BsrFI         202  GCCGGC               [GA]^CCGG[CT]
              208  ACCGGC              
BstUI         129  CGCG                 CG^CG
              189  CGCG                
BstYI         603  GGATCC               [GA]^GATC[CT]
             1125  GGATCC              
Cfr10I        202  GCCGGC               [GA]^CCGG[CT]
              208  ACCGGC              
EarI          700  GAAGAG               CTCTTC(1/4)
              787  GAAGAG              
EcoT14I       669  CCATGG               C^C[AT][AT]GG
             1065  CCTTGG              
HaeII           9  GGCGCC               [GA]GCGC^[CT]
              229  AGCGCC              
HgaI         1115  GACGC                GACGC(5/10)
              130  GCGTC               
Hin1I           9  GGCGCC               G[GA]^CG[CT]C
             1115  GACGCC              
HphI         1177  TCACC                GGTGA(8/7)
             1197  TCACC               
Ksp632I       700  GAAGAG               CTCTTC(1/4)
              787  GAAGAG              
MaeII         599  ACGT                 A^CGT
              658  ACGT                
MflI          603  GGATCC               [GA]^GATC[CT]
             1125  GGATCC              
MluNI         585  TGGCCA               TGG^CCA
              672  TGGCCA              
MscI          585  TGGCCA               TGG^CCA
              672  TGGCCA              
MslI          456  CATAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              813  CATCCCAGTG          
MvnI          129  CGCG                 CG^CG
              189  CGCG                
PvuII         893  CAGCTG               CAG^CTG
              922  CAGCTG              
StyI          669  CCATGG               C^C[AT][AT]GG
             1065  CCTTGG              
Tsp45I        410  GTCAC                ^GT[GC]AC
             1176  GTCAC               
XhoII         603  GGATCC               [GA]^GATC[CT]
             1125  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanII       BbvI        BmyI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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