Restriction map for pF1KB7451
 (Linear) (Six-base) MAPSORT of: KIBB7451.seq  Check: 3014  from: 1  to: 1248



KIBB7451.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     452     944    1248 
   Size:        452     492     304

  Fragments arranged by size: 

                 492     452     304
 
AflII C'TTAA_G

Cuts at:      0    1048    1248 
   Size:       1048     200
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     835    1248 
   Size:        835     413
 
ApoI r'AATT_y

Cuts at:      0     978    1248 
   Size:        978     270
 
AvaI C'yCGr_G

Cuts at:      0     540    1248 
   Size:        540     708
 
BamHI G'GATC_C

Cuts at:      0     603    1125    1248 
   Size:        603     522     123

  Fragments arranged by size: 

                 603     522     123
 
BanI G'GyrC_C

Cuts at:      0       9     205    1248 
   Size:          9     196    1043

  Fragments arranged by size: 

                1043     196       9
 
BanII G_rGCy'C

Cuts at:      0      21     245     794    1248 
   Size:         21     224     549     454

  Fragments arranged by size: 

                 549     454     224      21
 
BbeI G_GCGC'C

Cuts at:      0      13    1248 
   Size:         13    1235
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146    1248 
   Size:        146    1102
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     274     308    1248 
   Size:        274      34     940

  Fragments arranged by size: 

                 940     274      34
 
BlpI GC'TnA_GC

Cuts at:      0     244    1248 
   Size:        244    1004
 
Bme1580I G_kGCm'C

Cuts at:      0     824    1248 
   Size:        824     424
 
BmgBI CAC'GTC

Cuts at:      0     600    1248 
   Size:        600     648
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     811    1248 
   Size:        811     437
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     343    1248 
   Size:        343     905
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     772    1248 
   Size:        259     513     476

  Fragments arranged by size: 

                 513     476     259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     366    1248 
   Size:        123     243     882

  Fragments arranged by size: 

                 882     243     123
 
BsaHI Gr'CG_yC

Cuts at:      0      10    1116    1248 
   Size:         10    1106     132

  Fragments arranged by size: 

                1106     132      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     135     718     721     724     727    1248 
   Size:        135     583       3       3       3     521

  Fragments arranged by size: 

                 583     521     135       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     281     651    1248 
   Size:        281     370     597

  Fragments arranged by size: 

                 597     370     281
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     309    1108    1248 
   Size:         60     249     799     140

  Fragments arranged by size: 

                 799     249     140      60
 
BsiEI CG_ry'CG

Cuts at:      0     200    1248 
   Size:        200    1048
 
BsiHKAI G_wGCw'C

Cuts at:      0      21     245     407     794    1248 
   Size:         21     224     162     387     454

  Fragments arranged by size: 

                 454     387     224     162      21
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     993    1248 
   Size:        993     255
 
Bsp1286I G_dGCh'C

Cuts at:      0      21     245     407     794     824    1248 
   Size:         21     224     162     387      30     424

  Fragments arranged by size: 

                 424     387     224     162      30      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     542    1248 
   Size:        542     706
 
BsrBI CCG'CTC

Cuts at:      0    1169    1248 
   Size:       1169      79
 
BsrFI r'CCGG_y

Cuts at:      0     202     208    1248 
   Size:        202       6    1040

  Fragments arranged by size: 

                1040     202       6
 
BssSI C'ACGA_G

Cuts at:      0      14    1248 
   Size:         14    1234
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     471    1248 
   Size:        471     777
 
BstYI r'GATC_y

Cuts at:      0     603    1125    1248 
   Size:        603     522     123

  Fragments arranged by size: 

                 603     522     123
 
BtgI C'CryG_G

Cuts at:      0     669    1248 
   Size:        669     579
 
BtsI GCAGTG_nn'

Cuts at:      0     292     691     769    1248 
   Size:        292     399      78     479

  Fragments arranged by size: 

                 479     399     292      78
 
EaeI y'GGCC_r

Cuts at:      0     197     585     593     672    1248 
   Size:        197     388       8      79     576

  Fragments arranged by size: 

                 576     388     197      79       8
 
EagI C'GGCC_G

Cuts at:      0     197    1248 
   Size:        197    1051
 
EarI CTCTTCn'nnn_

Cuts at:      0     695     782    1248 
   Size:        695      87     466

  Fragments arranged by size: 

                 695     466      87
 
EcoICRI GAG'CTC

Cuts at:      0      19     243     792    1248 
   Size:         19     224     549     456

  Fragments arranged by size: 

                 549     456     224      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     772    1248 
   Size:        259     513     476

  Fragments arranged by size: 

                 513     476     259
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     501    1248 
   Size:        501     747
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1105    1248 
   Size:       1105     143
 
HaeII r_GCGC'y

Cuts at:      0      13     233    1248 
   Size:         13     220    1015

  Fragments arranged by size: 

                1015     220      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     595     627    1004    1036    1248 
   Size:        595      32     377      32     212

  Fragments arranged by size: 

                 595     377     212      32      32
 
HincII GTy'rAC

Cuts at:      0     614    1248 
   Size:        614     634
 
HindIII A'AGCT_T

Cuts at:      0     546    1248 
   Size:        546     702
 
KasI G'GCGC_C

Cuts at:      0       9    1248 
   Size:          9    1239
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     577    1248 
   Size:        362     215     671

  Fragments arranged by size: 

                 671     362     215
 
MscI TGG'CCA

Cuts at:      0     587     674    1248 
   Size:        587      87     574

  Fragments arranged by size: 

                 587     574      87
 
MslI CAynn'nnrTG

Cuts at:      0     460     817    1248 
   Size:        460     357     431

  Fragments arranged by size: 

                 460     431     357
 
MspA1I CmG'CkG

Cuts at:      0     166     895     924    1248 
   Size:        166     729      29     324

  Fragments arranged by size: 

                 729     324     166      29
 
NaeI GCC'GGC

Cuts at:      0     204    1248 
   Size:        204    1044
 
NarI GG'CG_CC

Cuts at:      0      10    1248 
   Size:         10    1238
 
NcoI C'CATG_G

Cuts at:      0     669    1248 
   Size:        669     579
 
NgoMIV G'CCGG_C

Cuts at:      0     202    1248 
   Size:        202    1046
 
NotI GC'GGCC_GC

Cuts at:      0     197    1248 
   Size:        197    1051
 
NspI r_CATG'y

Cuts at:      0    1145    1248 
   Size:       1145     103
 
PasI CC'CwG_GG

Cuts at:      0      93    1248 
   Size:         93    1155
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     750    1248 
   Size:        750     498
 
PfoI T'CCnGG_A

Cuts at:      0     329    1248 
   Size:        329     919
 
PpuMI rG'GwC_Cy

Cuts at:      0    1105    1248 
   Size:       1105     143
 
PstI C_TGCA'G

Cuts at:      0     863    1248 
   Size:        863     385
 
PvuII CAG'CTG

Cuts at:      0     895     924    1248 
   Size:        895      29     324

  Fragments arranged by size: 

                 895     324      29
 
SacI G_AGCT'C

Cuts at:      0      21     245     794    1248 
   Size:         21     224     549     454

  Fragments arranged by size: 

                 549     454     224      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0     782    1248 
   Size:        782     466
 
ScaI AGT'ACT

Cuts at:      0    1054    1248 
   Size:       1054     194
 
SfcI C'TryA_G

Cuts at:      0     859    1248 
   Size:        859     389
 
SfoI GGC'GCC

Cuts at:      0      11    1248 
   Size:         11    1237
 
SgrAI Cr'CCGG_yG

Cuts at:      0     208    1248 
   Size:        208    1040
 
SmlI C'TyrA_G

Cuts at:      0     102     381    1048    1248 
   Size:        102     279     667     200

  Fragments arranged by size: 

                 667     279     200     102
 
StyI C'CwwG_G

Cuts at:      0     669    1065    1248 
   Size:        669     396     183

  Fragments arranged by size: 

                 669     396     183
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     692    1248 
   Size:        692     556
 
TatI w'GTAC_w

Cuts at:      0    1052    1248 
   Size:       1052     196
 

 Enzymes that do cut:

     AccI    AflII     AlfI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstYI
     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NspI
     PasI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI     SapI
     ScaI     SfcI     SfoI    SgrAI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SgfI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com