Restriction map for pF1KB7444
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaI        
HpaII       KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          962  GTCGAC               GT^[AC][GT]AC
Alw44I       1205  GTGCAC               G^TGCAC
ApaLI        1205  GTGCAC               G^TGCAC
Asp700I       308  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          383  GGCACC               G^G[CT][GA]CC
BbvI          190  GCTGC                GCAGC(8/12)
BglII         328  AGATCT               A^GATCT
BpmI          606  CTGGAG               CTGGAG(16/14)
BsaBI         655  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          282  GAGACC               GGTCTC(1/5)
BseRI         482  CTCCTC               GAGGAG(10/8)
BsmI          186  GAATGC               GAATGC(1/-1)
BspHI        1107  TCATGA               T^CATGA
BsrI          445  CCAGT                ACTGG(1/-1)
BstAPI       1207  GCACCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         328  AGATCT               [GA]^GATC[CT]
Cac8I          23  GCAAGC               GC[ACGT]^[ACGT]GC
DsaI          933  CCATGG               C^C[GA][CT]GG
EarI          560  GAAGAG               CTCTTC(1/4)
EcoNI        1213  CCTGCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI        190  GCTGC                GC^[ACGT]GC
HindIII        25  AAGCTT               A^AGCTT
ItaI          190  GCTGC                GC^[ACGT]GC
Ksp632I       560  GAAGAG               CTCTTC(1/4)
MaeII         211  ACGT                 A^CGT
MamI          655  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          328  AGATCT               [GA]^GATC[CT]
NcoI          933  CCATGG               C^CATGG
NdeI         1409  CATATG               CA^TATG
PstI          173  CTGCAG               CTGCA^G
RcaI         1107  TCATGA               T^CATGA
SalI          962  GTCGAC               G^TCGAC
TfiI         1365  GAATC                G^A[AT]TC
TseI          190  GCTGC                G^C[AT]GC
Tsp45I        552  GTCAC                ^GT[GC]AC
Tsp509I       568  AATT                 ^AATT
TspRI         345  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         328  AGATCT               [GA]^GATC[CT]
XmnI          308  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI         1369  CCGC                 CCGC(-3/-1)
              279  GCGG                
AlwI         1396  GATCC                GGATC(4/5)
              123  GGATC               
AspHI        1205  GTGCAC               G[AT]GC[AT]^C
             1387  GTGCTC              
BsiHKAI      1205  GTGCAC               G[AT]GC[AT]^C
             1387  GTGCTC              
BspMI        1212  ACCTGC               ACCTGC(4/8)
             1249  ACCTGC              
BssSI        1271  CACGAG               CACGAG(-5/-1)
             1400  CTCGTG              
HincII        624  GTCAAC               GT[CT]^[GA]AC
              962  GTCGAC              
HindII        624  GTCAAC               GT[CT]^[GA]AC
              962  GTCGAC              
HphI         1120  GGTGA                GGTGA(8/7)
             1315  GGTGA               
MaeIII        552  GTCAC                ^GT[ACGT]AC
             1301  GTTAC               
MseI           71  TTAA                 T^TAA
              449  TTAA                
NspI          620  GCATGT               [GA]CATG^[CT]
             1341  GCATGT              
PaeR7I       1305  CTCGAG               C^TCGAG
             1476  CTCGAG              
SfcI          173  CTGCAG               C^T[GA][CT]AG
             1436  CTATAG              
Tru9I          71  TTAA                 T^TAA
              449  TTAA                
XhoI         1305  CTCGAG               C^TCGAG
             1476  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AluI        AvaI        AvaII       BciVI       BfaI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     EcoT22I     FokI        
HaeIII      HinfI       MaeI        MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NsiI        PleI        PpuMI       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TthHB8I     XbaI        
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