Restriction map for pF1KB7444
 (Linear) (Six-base) MAPSORT of: KIBB7444.seq  Check: 7812  from: 1  to: 1488



KIBB7444.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1221    1258    1488 
   Size:       1221      37     230

  Fragments arranged by size: 

                1221     230      37
 
AccI GT'mk_AC

Cuts at:      0     963    1488 
   Size:        963     525
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299    1165    1488 
   Size:        299     866     323

  Fragments arranged by size: 

                 866     323     299
 
ApaLI G'TGCA_C

Cuts at:      0    1205    1488 
   Size:       1205     283
 
AvaI C'yCGr_G

Cuts at:      0     785     896    1007    1305    1476    1488 
   Size:        785     111     111     298     171      12

  Fragments arranged by size: 

                 785     298     171     111     111      12
 
BanI G'GyrC_C

Cuts at:      0     383    1488 
   Size:        383    1105
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     394     868     931    1488 
   Size:        394     474      63     557

  Fragments arranged by size: 

                 557     474     394      63
 
BfrBI ATG'CAT

Cuts at:      0     715     826    1048    1488 
   Size:        715     111     222     440

  Fragments arranged by size: 

                 715     440     222     111
 
BglII A'GATC_T

Cuts at:      0     328    1488 
   Size:        328    1160
 
Bme1580I G_kGCm'C

Cuts at:      0     386    1209    1488 
   Size:        386     823     279

  Fragments arranged by size: 

                 823     386     279
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1320    1488 
   Size:       1320     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     627    1488 
   Size:        627     861
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400    1404    1488 
   Size:        400    1004      84

  Fragments arranged by size: 

                1004     400      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     276    1488 
   Size:        276    1212
 
BsaBI GATnn'nnATC

Cuts at:      0     659    1488 
   Size:        659     829
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     473    1488 
   Size:        473    1015
 
BsiHKAI G_wGCw'C

Cuts at:      0    1209    1391    1488 
   Size:       1209     182      97

  Fragments arranged by size: 

                1209     182      97
 
BsmI GAATG_Cn'

Cuts at:      0     192    1488 
   Size:        192    1296
 
Bsp1286I G_dGCh'C

Cuts at:      0     386    1209    1391    1488 
   Size:        386     823     182      97

  Fragments arranged by size: 

                 823     386     182      97
 
BspHI T'CATG_A

Cuts at:      0    1107    1488 
   Size:       1107     381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1221    1258    1488 
   Size:       1221      37     230

  Fragments arranged by size: 

                1221     230      37
 
BssSI C'ACGA_G

Cuts at:      0    1271    1400    1488 
   Size:       1271     129      88

  Fragments arranged by size: 

                1271     129      88
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1213    1488 
   Size:       1213     275
 
BstYI r'GATC_y

Cuts at:      0     328    1488 
   Size:        328    1160
 
BtgI C'CryG_G

Cuts at:      0     933    1488 
   Size:        933     555
 
BtsI GCAGTG_nn'

Cuts at:      0     351    1488 
   Size:        351    1137
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1409    1444    1488 
   Size:       1409      35      44

  Fragments arranged by size: 

                1409      44      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     555    1488 
   Size:        555     933
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     294    1358    1488 
   Size:        294    1064     130

  Fragments arranged by size: 

                1064     294     130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     627    1165    1488 
   Size:        299     328     538     323

  Fragments arranged by size: 

                 538     328     323     299
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1217    1488 
   Size:       1217     271
 
EcoO109I rG'GnC_Cy

Cuts at:      0     478     544    1125    1193    1222    1488 
   Size:        478      66     581      68      29     266

  Fragments arranged by size: 

                 581     478     266      68      66      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140     600     607     632     639    1444    1476 
   Size:        108      32     460       7      25       7     805      32

Cuts at:   1476    1488 
   Size:         12

  Fragments arranged by size: 

                 805     460     108      32      32      25      12       7
                   7
 
HincII GTy'rAC

Cuts at:      0     626     964    1488 
   Size:        626     338     524

  Fragments arranged by size: 

                 626     524     338
 
HindIII A'AGCT_T

Cuts at:      0      25    1488 
   Size:         25    1463
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     508    1488 
   Size:        508     980
 
MslI CAynn'nnrTG

Cuts at:      0     389    1154    1328    1488 
   Size:        389     765     174     160

  Fragments arranged by size: 

                 765     389     174     160
 
NcoI C'CATG_G

Cuts at:      0     933    1488 
   Size:        933     555
 
NdeI CA'TA_TG

Cuts at:      0    1410    1488 
   Size:       1410      78
 
NsiI A_TGCA'T

Cuts at:      0     717     828    1050    1488 
   Size:        717     111     222     438

  Fragments arranged by size: 

                 717     438     222     111
 
NspI r_CATG'y

Cuts at:      0     624    1345    1488 
   Size:        624     721     143

  Fragments arranged by size: 

                 721     624     143
 
PfoI T'CCnGG_A

Cuts at:      0     604    1488 
   Size:        604     884
 
PpuMI rG'GwC_Cy

Cuts at:      0     478     544    1193    1488 
   Size:        478      66     649     295

  Fragments arranged by size: 

                 649     478     295      66
 
PspXI vC'TCGA_Gb

Cuts at:      0    1305    1476    1488 
   Size:       1305     171      12

  Fragments arranged by size: 

                1305     171      12
 
PstI C_TGCA'G

Cuts at:      0     177    1488 
   Size:        177    1311
 
SalI G'TCGA_C

Cuts at:      0     962    1488 
   Size:        962     526
 
SfcI C'TryA_G

Cuts at:      0     173    1436    1488 
   Size:        173    1263      52

  Fragments arranged by size: 

                1263     173      52
 
SmlI C'TyrA_G

Cuts at:      0     415    1305    1419    1476    1488 
   Size:        415     890     114      57      12

  Fragments arranged by size: 

                 890     415     114      57      12
 
StyI C'CwwG_G

Cuts at:      0     540     933    1128    1488 
   Size:        540     393     195     360

  Fragments arranged by size: 

                 540     393     360     195
 
XbaI T'CTAG_A

Cuts at:      0     703     757     814     868     925     979    1090    1488 
   Size:        703      54      57      54      57      54     111     398

  Fragments arranged by size: 

                 703     398     111      57      57      54      54      54
            
 
XhoI C'TCGA_G

Cuts at:      0    1305    1476    1488 
   Size:       1305     171      12

  Fragments arranged by size: 

                1305     171      12
 
XmnI GAAnn'nnTTC

Cuts at:      0     312    1488 
   Size:        312    1176
 

 Enzymes that do cut:

     AarI     AccI     AcuI    ApaLI     AvaI     BanI    BciVI    BfrBI
    BglII Bme1580I     BplI     BpmI    BpuEI     BsaI    BsaBI    BseRI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BssSI   BstAPI    BstYI
     BtgI     BtsI    CspCI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    Hin4I   HincII  HindIII     MmeI     MslI     NcoI     NdeI     NsiI
     NspI     PfoI    PpuMI    PspXI     PstI     SalI     SfcI     SmlI
     StyI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI     BlpI    BmgBI     BmrI     BmtI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     ZraI
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