Restriction map for pF1KB7442
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AciI        
AclI        AflIII      AgeI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsoBI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      CpoI        
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MluI        MroI        MslI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        StuI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         527  GACGTC               GACGT^C
AcsI         1021  AAATTT               [GA]^AATT[CT]
AcyI          527  GACGTC               G[GA]^CG[CT]C
AflII         717  CTTAAG               C^TTAAG
AhdI          522  GACCAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        259  GTGCAC               G^TGCAC
AlwNI         803  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         259  GTGCAC               G^TGCAC
ApoI         1021  AAATTT               [GA]^AATT[CT]
Asp700I       105  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         522  GACCAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          359  TGGCCA               TGG^CCA
BbsI          927  GAAGAC               GAAGAC(2/6)
BfaI          626  CTAG                 C^TAG
BfrI          717  CTTAAG               C^TTAAG
BpuAI         927  GAAGAC               GAAGAC(2/6)
BsaBI         585  GATTCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         527  GACGTC               G[GA]^CG[CT]C
BsaI          839  GGTCTC               GGTCTC(1/5)
BsmBI         529  CGTCTC               CGTCTC(1/5)
BsmI          445  GAATGC               GAATGC(1/-1)
Bsp1407I      290  TGTACA               T^GTACA
BspDI         476  ATCGAT               AT^CGAT
BsrDI         236  GCAATG               GCAATG(2/0)
BsrGI         290  TGTACA               T^GTACA
ClaI          476  ATCGAT               AT^CGAT
DraI           24  TTTAAA               TTT^AAA
EaeI          359  TGGCCA               [CT]^GGCC[GA]
Eam1105I      522  GACCAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       431  CCTTGG               C^C[AT][AT]GG
EcoT22I         1  ATGCAT               ATGCA^T
Hin1I         527  GACGTC               G[GA]^CG[CT]C
HincII        595  GTCAAC               GT[CT]^[GA]AC
HindII        595  GTCAAC               GT[CT]^[GA]AC
MaeI          626  CTAG                 C^TAG
MaeII         528  ACGT                 A^CGT
MamI          585  GATTCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          552  CAATTG               C^AATTG
MluNI         359  TGGCCA               TGG^CCA
MscI          359  TGGCCA               TGG^CCA
MspA1I        806  CAGCTG               C[AC]G^C[GT]G
MunI          552  CAATTG               C^AATTG
NsiI            1  ATGCAT               ATGCA^T
NspI          353  GCATGC               [GA]CATG^[CT]
PvuII         806  CAGCTG               CAG^CTG
SapI          201  GCTCTTC              GCTCTTC(1/4)
ScaI          876  AGTACT               AGT^ACT
SfcI          440  CTACAG               C^T[GA][CT]AG
SmlI          717  CTTAAG               C^T[CT][GA]AG
SphI          353  GCATGC               GCATG^C
SspBI         290  TGTACA               T^GTACA
StyI          431  CCTTGG               C^C[AT][AT]GG
SwaI           23  ATTTAAAT             ATTT^AAAT
TaqI          477  TCGA                 T^CGA
TthHB8I       477  TCGA                 T^CGA
XmnI          105  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AspHI         199  GTGCTC               G[AT]GC[AT]^C
              259  GTGCAC              
AvaII         761  GGTCC                G^G[AT]CC
              795  GGTCC               
BanII         435  GGGCTC               G[GA]GC[CT]^C
              894  GAGCCC              
BbvI          805  GCAGC                GCAGC(8/12)
              401  GCTGC               
BpmI          129  CTCCAG               CTGGAG(16/14)
              409  CTCCAG              
BseRI         190  CTCCTC               GAGGAG(10/8)
              659  CTCCTC              
BsiHKAI       199  GTGCTC               G[AT]GC[AT]^C
              259  GTGCAC              
BsmFI         346  GTCCC                GGGAC(10/14)
              796  GTCCC               
BstF5I        150  CATCC                GGATG(2/0)
              294  CATCC               
BstXI         350  CCAGCATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              539  CCAATCTTGTGG        
BstYI         185  AGATCC               [GA]^GATC[CT]
              622  GGATCT              
Fnu4HI        401  GCTGC                GC^[ACGT]GC
              805  GCAGC               
FokI          150  CATCC                GGATG(9/13)
              294  CATCC               
HphI          335  TCACC                GGTGA(8/7)
              843  TCACC               
ItaI          401  GCTGC                GC^[ACGT]GC
              805  GCAGC               
MflI          185  AGATCC               [GA]^GATC[CT]
              622  GGATCT              
PleI          275  GACTC                GAGTC(4/5)
              936  GACTC               
PpuMI         760  GGGTCCT              [GA]G^G[AT]CC[CT]
              794  GGGTCCC             
TfiI           39  GATTC                G^A[AT]TC
              585  GATTC               
TseI          401  GCTGC                G^C[AT]GC
              805  GCAGC               
XhoII         185  AGATCC               [GA]^GATC[CT]
              622  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AfaI        AluI        AlwI        BmyI        BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
HaeIII      HinfI       Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       Tru9I       Tsp45I      Tsp509I     
TspRI       
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