Restriction map for pF1KB7442
 (Linear) (Six-base) MAPSORT of: KIBB7442.seq  Check: 9743  from: 1  to: 1035



KIBB7442.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AatII G_ACGT'C

Cuts at:      0     531    1035 
   Size:        531     504
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785    1035 
   Size:        785     250
 
AflII C'TTAA_G

Cuts at:      0     717    1035 
   Size:        717     318
 
AhdI GACnn_n'nnGTC

Cuts at:      0     527    1035 
   Size:        527     508
 
AlwNI CAG_nnn'CTG

Cuts at:      0     808    1035 
   Size:        808     227
 
ApaLI G'TGCA_C

Cuts at:      0     259    1035 
   Size:        259     776
 
ApoI r'AATT_y

Cuts at:      0    1021    1035 
   Size:       1021      14
 
BanII G_rGCy'C

Cuts at:      0     439     898    1035 
   Size:        439     459     137

  Fragments arranged by size: 

                 459     439     137
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     934    1035 
   Size:        934     101
 
BfrBI ATG'CAT

Cuts at:      0       3    1035 
   Size:          3    1032
 
Bme1580I G_kGCm'C

Cuts at:      0     263    1035 
   Size:        263     772
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     273    1035 
   Size:        273     762
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1028    1035 
   Size:       1028       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     394    1035 
   Size:        114     280     641

  Fragments arranged by size: 

                 641     280     114
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     845    1035 
   Size:        845     190
 
BsaBI GATnn'nnATC

Cuts at:      0     589    1035 
   Size:        589     446
 
BsaHI Gr'CG_yC

Cuts at:      0     528    1035 
   Size:        528     507
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181     650    1035 
   Size:        181     469     385

  Fragments arranged by size: 

                 469     385     181
 
BseYI C'CCAG_C

Cuts at:      0     121     349     557     654     808    1035 
   Size:        121     228     208      97     154     227

  Fragments arranged by size: 

                 228     227     208     154     121      97
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     263    1035 
   Size:        203      60     772

  Fragments arranged by size: 

                 772     203      60
 
BsmI GAATG_Cn'

Cuts at:      0     451    1035 
   Size:        451     584
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     535    1035 
   Size:        535     500
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     263     439     898    1035 
   Size:        203      60     176     459     137

  Fragments arranged by size: 

                 459     203     176     137      60
 
BsrDI GCAATG_nn'

Cuts at:      0     243    1035 
   Size:        243     792
 
BsrGI T'GTAC_A

Cuts at:      0     290    1035 
   Size:        290     745
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     357     546    1035 
   Size:        357     189     489

  Fragments arranged by size: 

                 489     357     189
 
BstYI r'GATC_y

Cuts at:      0     185     622    1035 
   Size:        185     437     413

  Fragments arranged by size: 

                 437     413     185
 
BtsI GCAGTG_nn'

Cuts at:      0     961    1035 
   Size:        961      74
 
ClaI AT'CG_AT

Cuts at:      0     477    1035 
   Size:        477     558
 
DraI TTT'AAA

Cuts at:      0      26    1035 
   Size:         26    1009
 
EaeI y'GGCC_r

Cuts at:      0     359    1035 
   Size:        359     676
 
EarI CTCTTCn'nnn_

Cuts at:      0     208     668     754    1035 
   Size:        208     460      86     281

  Fragments arranged by size: 

                 460     281     208      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     394     785    1035 
   Size:        114     280     391     250

  Fragments arranged by size: 

                 391     280     250     114
 
EcoO109I rG'GnC_Cy

Cuts at:      0     688     761     795    1035 
   Size:        688      73      34     240

  Fragments arranged by size: 

                 688     240      73      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     839    1035 
   Size:        839     196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30      62     513     545     921     953     969    1001 
   Size:         30      32     451      32     376      32      16      32

Cuts at:   1001    1035 
   Size:         34

  Fragments arranged by size: 

                 451     376      34      32      32      32      32      30
                  16
 
HincII GTy'rAC

Cuts at:      0     597    1035 
   Size:        597     438
 
MfeI C'AATT_G

Cuts at:      0     552    1035 
   Size:        552     483
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     535    1035 
   Size:        535     500
 
MscI TGG'CCA

Cuts at:      0     361    1035 
   Size:        361     674
 
MspA1I CmG'CkG

Cuts at:      0     808    1035 
   Size:        808     227
 
NsiI A_TGCA'T

Cuts at:      0       5    1035 
   Size:          5    1030
 
NspI r_CATG'y

Cuts at:      0     357    1035 
   Size:        357     678
 
PasI CC'CwG_GG

Cuts at:      0     173    1035 
   Size:        173     862
 
PpuMI rG'GwC_Cy

Cuts at:      0     761     795    1035 
   Size:        761      34     240

  Fragments arranged by size: 

                 761     240      34
 
PvuII CAG'CTG

Cuts at:      0     808    1035 
   Size:        808     227
 
SanDI GG'GwC_CC

Cuts at:      0     795    1035 
   Size:        795     240
 
SapI GCTCTTCn'nnn_

Cuts at:      0     208    1035 
   Size:        208     827
 
ScaI AGT'ACT

Cuts at:      0     878    1035 
   Size:        878     157
 
SfcI C'TryA_G

Cuts at:      0     440    1035 
   Size:        440     595
 
SmlI C'TyrA_G

Cuts at:      0     717    1035 
   Size:        717     318
 
SphI G_CATG'C

Cuts at:      0     357    1035 
   Size:        357     678
 
StyI C'CwwG_G

Cuts at:      0     431    1035 
   Size:        431     604
 
SwaI ATTT'AAAT

Cuts at:      0      26    1035 
   Size:         26    1009
 
TatI w'GTAC_w

Cuts at:      0     290     876    1035 
   Size:        290     586     159

  Fragments arranged by size: 

                 586     290     159
 
XmnI GAAnn'nnTTC

Cuts at:      0     109    1035 
   Size:        109     926
 
ZraI GAC'GTC

Cuts at:      0     529    1035 
   Size:        529     506
 

 Enzymes that do cut:

    AatII     AcuI    AflII     AhdI    AlwNI    ApaLI     ApoI    BanII
     BbsI    BfrBI Bme1580I     BmrI     BplI     BpmI     BsaI    BsaBI
    BsaHI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrDI
    BsrGI    BstXI    BstYI     BtsI     ClaI     DraI     EaeI     EarI
  Eco57MI EcoO109I     FalI    Hin4I   HincII     MfeI     MmeI     MscI
   MspA1I     NsiI     NspI     PasI    PpuMI    PvuII    SanDI     SapI
     ScaI     SfcI     SmlI     SphI     StyI     SwaI     TatI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AleI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI
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