Restriction map for pF1KB7441
 (Linear) (Six-base) MAPSORT of: KIBB7441.seq  Check: 6201  from: 1  to: 645



KIBB7441.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AatII G_ACGT'C

Cuts at:      0     599     645 
   Size:        599      46
 
AccI GT'mk_AC

Cuts at:      0     173     645 
   Size:        173     472
 
AflIII A'CryG_T

Cuts at:      0      54     645 
   Size:         54     591
 
AleI CACnn'nnGTG

Cuts at:      0      55     645 
   Size:         55     590
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      47     645 
   Size:         47     598
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     364     396     645 
   Size:        364      32     249

  Fragments arranged by size: 

                 364     249      32
 
ApaLI G'TGCA_C

Cuts at:      0       3      58     645 
   Size:          3      55     587

  Fragments arranged by size: 

                 587      55       3
 
AseI AT'TA_AT

Cuts at:      0     614     645 
   Size:        614      31
 
BanII G_rGCy'C

Cuts at:      0     100     593     645 
   Size:        100     493      52

  Fragments arranged by size: 

                 493     100      52
 
BclI T'GATC_A

Cuts at:      0     546     645 
   Size:        546      99
 
BglII A'GATC_T

Cuts at:      0      28     645 
   Size:         28     617
 
Bme1580I G_kGCm'C

Cuts at:      0       7      62     645 
   Size:          7      55     583

  Fragments arranged by size: 

                 583      55       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     645 
   Size:        574      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     263     645 
   Size:        263     382
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     435     645 
   Size:        435     210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     190     645 
   Size:        190     455
 
BsaAI yAC'GTr

Cuts at:      0     369     645 
   Size:        369     276
 
BsaHI Gr'CG_yC

Cuts at:      0     596     645 
   Size:        596      49
 
BsiHKAI G_wGCw'C

Cuts at:      0       7      62     169     593     645 
   Size:          7      55     107     424      52

  Fragments arranged by size: 

                 424     107      55      52       7
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      62     100     169     593     645 
   Size:          7      55      38      69     424      52

  Fragments arranged by size: 

                 424      69      55      52      38       7
 
BspHI T'CATG_A

Cuts at:      0     624     645 
   Size:        624      21
 
BsrDI GCAATG_nn'

Cuts at:      0     431     478     645 
   Size:        431      47     167

  Fragments arranged by size: 

                 431     167      47
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      92     645 
   Size:         92     553
 
BstYI r'GATC_y

Cuts at:      0      28     645 
   Size:         28     617
 
BtgI C'CryG_G

Cuts at:      0     106     180     645 
   Size:        106      74     465

  Fragments arranged by size: 

                 465     106      74
 
BtsI GCAGTG_nn'

Cuts at:      0     495     496     645 
   Size:        495       1     149

  Fragments arranged by size: 

                 495     149       1
 
EarI CTCTTCn'nnn_

Cuts at:      0     221     645 
   Size:        221     424
 
EcoICRI GAG'CTC

Cuts at:      0     591     645 
   Size:        591      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     645 
   Size:        574      71
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     288     645 
   Size:        288     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     477     509     645 
   Size:        477      32     136

  Fragments arranged by size: 

                 477     136      32
 
MslI CAynn'nnrTG

Cuts at:      0      55      71      77     211     645 
   Size:         55      16       6     134     434

  Fragments arranged by size: 

                 434     134      55      16       6
 
MspA1I CmG'CkG

Cuts at:      0     552     645 
   Size:        552      93
 
NspI r_CATG'y

Cuts at:      0      58     216     456     645 
   Size:         58     158     240     189

  Fragments arranged by size: 

                 240     189     158      58
 
PasI CC'CwG_GG

Cuts at:      0     582     645 
   Size:        582      63
 
PciI A'CATG_T

Cuts at:      0      54     645 
   Size:         54     591
 
PstI C_TGCA'G

Cuts at:      0     314     502     645 
   Size:        314     188     143

  Fragments arranged by size: 

                 314     188     143
 
PvuII CAG'CTG

Cuts at:      0     552     645 
   Size:        552      93
 
SacI G_AGCT'C

Cuts at:      0     593     645 
   Size:        593      52
 
SfcI C'TryA_G

Cuts at:      0     310     498     645 
   Size:        310     188     147

  Fragments arranged by size: 

                 310     188     147
 
SmlI C'TyrA_G

Cuts at:      0     414     645 
   Size:        414     231
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     354     579     645 
   Size:        354     225      66

  Fragments arranged by size: 

                 354     225      66
 
ZraI GAC'GTC

Cuts at:      0     597     645 
   Size:        597      48
 

 Enzymes that do cut:

    AatII     AccI   AflIII     AleI     AlfI     AloI    ApaLI     AseI
    BanII     BclI    BglII Bme1580I     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI  BsiHKAI Bsp1286I    BspHI    BsrDI    BstXI    BstYI
     BtgI     BtsI     EarI  EcoICRI  Eco57MI     FalI    Hin4I     MslI
   MspA1I     NspI     PasI     PciI     PstI    PvuII     SacI     SfcI
     SmlI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
    AlwNI     ApaI     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com