Restriction map for pF1KB7440
 (Linear) (Six-base) MAPSORT of: KIBB7440.seq  Check: 3795  from: 1  to: 456



KIBB7440.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     456 
   Size:        331     125
 
AflIII A'CryG_T

Cuts at:      0     368     456 
   Size:        368      88
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     416     456 
   Size:        416      40
 
AvaI C'yCGr_G

Cuts at:      0     146     456 
   Size:        146     310
 
BanI G'GyrC_C

Cuts at:      0     142     456 
   Size:        142     314
 
BanII G_rGCy'C

Cuts at:      0     389     456 
   Size:        389      67
 
BbeI G_GCGC'C

Cuts at:      0     146     456 
   Size:        146     310
 
Bme1580I G_kGCm'C

Cuts at:      0     441     456 
   Size:        441      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     456 
   Size:        265     191
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     456 
   Size:         64     392
 
BsaAI yAC'GTr

Cuts at:      0     442     456 
   Size:        442      14
 
BsaHI Gr'CG_yC

Cuts at:      0     143     456 
   Size:        143     313
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     274     304     456 
   Size:        274      30     152

  Fragments arranged by size: 

                 274     152      30
 
BseYI C'CCAG_C

Cuts at:      0     151     168     456 
   Size:        151      17     288

  Fragments arranged by size: 

                 288     151      17
 
BsiHKAI G_wGCw'C

Cuts at:      0     389     456 
   Size:        389      67
 
BsiWI C'GTAC_G

Cuts at:      0      68     456 
   Size:         68     388
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     441     456 
   Size:        389      52      15

  Fragments arranged by size: 

                 389      52      15
 
BsrFI r'CCGG_y

Cuts at:      0     139     456 
   Size:        139     317
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     434     456 
   Size:        434      22
 
BtgI C'CryG_G

Cuts at:      0     197     456 
   Size:        197     259
 
EaeI y'GGCC_r

Cuts at:      0     137     320     456 
   Size:        137     183     136

  Fragments arranged by size: 

                 183     137     136
 
EarI CTCTTCn'nnn_

Cuts at:      0     377     456 
   Size:        377      79
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      65     100     265     456 
   Size:         65      35     165     191

  Fragments arranged by size: 

                 191     165      65      35
 
EcoICRI GAG'CTC

Cuts at:      0     387     456 
   Size:        387      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     331     456 
   Size:        265      66     125

  Fragments arranged by size: 

                 265     125      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      78     456 
   Size:         78     378
 
EcoO109I rG'GnC_Cy

Cuts at:      0     240     456 
   Size:        240     216
 
HaeII r_GCGC'y

Cuts at:      0     146     212     456 
   Size:        146      66     244

  Fragments arranged by size: 

                 244     146      66
 
HincII GTy'rAC

Cuts at:      0      65     456 
   Size:         65     391
 
KasI G'GCGC_C

Cuts at:      0     142     456 
   Size:        142     314
 
MluI A'CGCG_T

Cuts at:      0     368     456 
   Size:        368      88
 
MslI CAynn'nnrTG

Cuts at:      0     398     456 
   Size:        398      58
 
MspA1I CmG'CkG

Cuts at:      0      26     103     185     237     342     456 
   Size:         26      77      82      52     105     114

  Fragments arranged by size: 

                 114     105      82      77      52      26
 
NaeI GCC'GGC

Cuts at:      0     141     456 
   Size:        141     315
 
NarI GG'CG_CC

Cuts at:      0     143     456 
   Size:        143     313
 
NcoI C'CATG_G

Cuts at:      0     197     456 
   Size:        197     259
 
NgoMIV G'CCGG_C

Cuts at:      0     139     456 
   Size:        139     317
 
NspI r_CATG'y

Cuts at:      0     259     456 
   Size:        259     197
 
PmlI CAC'GTG

Cuts at:      0     442     456 
   Size:        442      14
 
PpuMI rG'GwC_Cy

Cuts at:      0     240     456 
   Size:        240     216
 
PstI C_TGCA'G

Cuts at:      0     236     456 
   Size:        236     220
 
PvuII CAG'CTG

Cuts at:      0     185     456 
   Size:        185     271
 
SacI G_AGCT'C

Cuts at:      0     389     456 
   Size:        389      67
 
SapI GCTCTTCn'nnn_

Cuts at:      0     377     456 
   Size:        377      79
 
ScaI AGT'ACT

Cuts at:      0      57     456 
   Size:         57     399
 
SfcI C'TryA_G

Cuts at:      0     232     294     456 
   Size:        232      62     162

  Fragments arranged by size: 

                 232     162      62
 
SfoI GGC'GCC

Cuts at:      0     144     456 
   Size:        144     312
 
SmlI C'TyrA_G

Cuts at:      0      43     456 
   Size:         43     413
 
StyI C'CwwG_G

Cuts at:      0     197     456 
   Size:        197     259
 
TatI w'GTAC_w

Cuts at:      0      55     456 
   Size:         55     401
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     AvaI     BanI    BanII     BbeI Bme1580I
     BpmI    BpuEI    BsaAI    BsaHI    BsaXI    BseYI  BsiHKAI    BsiWI
 Bsp1286I    BsrFI    BstXI     BtgI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII   HincII     KasI     MluI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PmlI    PpuMI
     PstI    PvuII     SacI     SapI     ScaI     SfcI     SfoI     SmlI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaWI    BseRI     BsgI
    BsiEI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
  HindIII     HpaI     KpnI     MfeI     MmeI     MscI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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