Restriction map for pF1KB7437
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmAI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrI        BssHII      BssSI       BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        KasI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvaI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I         20  GGTACC               G^GTACC
AcsI          451  AAATTT               [GA]^AATT[CT]
Alw44I         82  GTGCAC               G^TGCAC
AlwI          235  GGATC                GGATC(4/5)
ApaLI          82  GTGCAC               G^TGCAC
ApoI          451  AAATTT               [GA]^AATT[CT]
Asp700I       438  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        20  GGTACC               G^GTACC
AspHI          82  GTGCAC               G[AT]GC[AT]^C
BanI           20  GGTACC               G^G[CT][GA]CC
BanII         557  GAGCCC               G[GA]GC[CT]^C
BciVI         279  GTATCC               GTATCC(6/5)
BcnI           17  CCCGG                CC^[GC]GG
BsaAI         628  TACGTA               [CT]AC^GT[GA]
BseRI          30  CTCCTC               GAGGAG(10/8)
BsgI          676  CTGCAC               GTGCAG(16/14)
BsiEI          35  CGATCG               CG[GA][CT]^CG
BsiHKAI        82  GTGCAC               G[AT]GC[AT]^C
BsmI          119  GCATTC               GAATGC(1/-1)
Bsp1407I      195  TGTACA               T^GTACA
BspMI         132  GCAGGT               ACCTGC(4/8)
BsrGI         195  TGTACA               T^GTACA
BssKI          17  CCCGG                ^CC[ACGT]GG
Bst1107I      420  GTATAC               GTA^TAC
BstF5I         87  CATCC                GGATG(2/0)
BstYI         235  GGATCT               [GA]^GATC[CT]
BstZ17I       420  GTATAC               GTA^TAC
Cac8I         375  GCAAGC               GC[ACGT]^[ACGT]GC
DraI          264  TTTAAA               TTT^AAA
EarI          622  GAAGAG               CTCTTC(1/4)
EcoRV         658  GATATC               GAT^ATC
EcoT22I       604  ATGCAT               ATGCA^T
FokI           87  CATCC                GGATG(9/13)
HapII          18  CCGG                 C^CGG
HindIII       377  AAGCTT               A^AGCTT
HpaII          18  CCGG                 C^CGG
HphI          616  TCACC                GGTGA(8/7)
KpnI           20  GGTACC               GGTAC^C
Ksp632I       622  GAAGAG               CTCTTC(1/4)
MaeII         629  ACGT                 A^CGT
MboII         622  GAAGA                GAAGA(8/7)
MflI          235  GGATCT               [GA]^GATC[CT]
MslI           87  CATCCGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        548  CCGCTG               C[AC]G^C[GT]G
MspI           18  CCGG                 C^CGG
MwoI          550  GCTGCAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           17  CCCGG                CC^[GC]GG
NlaIII        199  CATG                 CATG^
NsiI          604  ATGCAT               ATGCA^T
PvuI           35  CGATCG               CGAT^CG
ScrFI          17  CCCGG                CC^[ACGT]GG
SfaNI         603  GATGC                GCATC(5/9)
SnaBI         628  TACGTA               TAC^GTA
SspBI         195  TGTACA               T^GTACA
XhoII         235  GGATCT               [GA]^GATC[CT]
XmnI          438  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI          112  GTCTAC               GT^[AC][GT]AC
              420  GTATAC              
AciI           74  CCGC                 CCGC(-3/-1)
              548  CCGC                
BmyI           82  GTGCAC               G[AGT]GC[ACT]^C
              557  GAGCCC              
BsaJI          16  CCCCGG               C^C[ACGT][ACGT]GG
              231  CCTCGG              
BsiYI          11  CCGACCCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              648  CCTCTACTTGG         
BslI           11  CCGACCCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              648  CCTCTACTTGG         
Bsp1286I       82  GTGCAC               G[AGT]GC[ACT]^C
              557  GAGCCC              
DpnII          36  GATC                 ^GATC
              236  GATC                
HaeIII         69  GGCC                 GG^CC
               96  GGCC                
MboI           36  GATC                 ^GATC
              236  GATC                
NdeII          36  GATC                 ^GATC
              236  GATC                
NlaIV          20  GGTACC               GG[ACGT]^[ACGT]CC
              556  GGAGCC              
PleI          178  GAGTC                GAGTC(4/5)
              204  GAGTC               
PstI          130  CTGCAG               CTGCA^G
              551  CTGCAG              
Sau3AI         36  GATC                 ^GATC
              236  GATC                
TaqI           34  TCGA                 T^CGA
               38  TCGA                
TfiI          500  GATTC                G^A[AT]TC
              575  GATTC               
Tsp45I        151  GTGAC                ^GT[GC]AC
              615  GTCAC               
Tsp509I       216  AATT                 ^AATT
              452  AATT                
TspRI         149  CAGTG                CA[GC]TG[ACGT][ACGT]^
              599  CACTG               
TthHB8I        34  TCGA                 T^CGA
               38  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AfaI        AluI        BbvI        DdeI        Fnu4HI      
HinfI       ItaI        MaeIII      MnlI        MseI        
RsaI        SfcI        Tru9I       TseI        
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