Restriction map for pF1KB7435
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        NarI        NciI        NcoI        
NdeI        NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AcsI           74  GAATTC               [GA]^AATT[CT]
AflIII        229  ACATGT               A^C[GA][CT]GT
ApoI           74  GAATTC               [GA]^AATT[CT]
AspHI          31  GAGCTC               G[AT]GC[AT]^C
BanII          31  GAGCTC               G[GA]GC[CT]^C
BbvI          357  GCAGC                GCAGC(8/12)
BfaI          142  CTAG                 C^TAG
BglI          124  GCCACCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           31  GAGCTC               G[AGT]GC[ACT]^C
BsaBI          82  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI          28  GAGGAG               GAGGAG(10/8)
BsgI           58  GTGCAG               GTGCAG(16/14)
BsiHKAI        31  GAGCTC               G[AT]GC[AT]^C
BsmAI         367  GTCTC                GTCTC(1/5)
BsmI          271  GCATTC               GAATGC(1/-1)
Bsp1286I       31  GAGCTC               G[AGT]GC[ACT]^C
BspLU11I      229  ACATGT               A^CATGT
BsrFI          15  GCCGGC               [GA]^CCGG[CT]
BssHII         68  GCGCGC               G^CGCGC
BstAPI        299  GCATCCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        300  CATCC                GGATG(2/0)
BstXI         198  CCAGGTCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         15  GCCGGC               [GA]^CCGG[CT]
DpnII         148  GATC                 ^GATC
DraIII        176  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EarI          288  GAAGAG               CTCTTC(1/4)
EcoNI         330  CCTGAGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          74  GAATTC               G^AATTC
FokI          300  CATCC                GGATG(9/13)
HapII          16  CCGG                 C^CGG
HincII        381  GTTGAC               GT[CT]^[GA]AC
HindII        381  GTTGAC               GT[CT]^[GA]AC
HinfI         333  GAGTC                G^A[ACGT]TC
HpaII          16  CCGG                 C^CGG
Ksp632I       288  GAAGAG               CTCTTC(1/4)
MaeI          142  CTAG                 C^TAG
MaeIII        293  GTGAC                ^GT[ACGT]AC
MamI           82  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          148  GATC                 ^GATC
MseI          166  TTAA                 T^TAA
MslI          224  CATACACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           16  CCGG                 C^CGG
NaeI           15  GCCGGC               GCC^GGC
NdeII         148  GATC                 ^GATC
NgoMIV         15  GCCGGC               G^CCGGC
NspI          229  ACATGT               [GA]CATG^[CT]
PflMI         129  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          333  GAGTC                GAGTC(4/5)
SacI           31  GAGCTC               GAGCT^C
Sau3AI        148  GATC                 ^GATC
SexAI         197  ACCAGGT              A^CC[AT]GGT
StuI          131  AGGCCT               AGG^CCT
TaqI          150  TCGA                 T^CGA
Tru9I         166  TTAA                 T^TAA
TseI          357  GCAGC                G^C[AT]GC
Tsp45I        293  GTGAC                ^GT[GC]AC
Tsp509I        75  AATT                 ^AATT
TthHB8I       150  TCGA                 T^CGA
Van91I        129  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          125  CCACCCAGGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          69  CGCG                 CG^CG
               71  CGCG                
AluI           32  AGCT                 AG^CT
              359  AGCT                
BsrI          179  CCAGT                ACTGG(1/-1)
              215  CCAGT               
BssKI         129  CCAGG                ^CC[ACGT]GG
              198  CCAGG               
BstNI         129  CCAGG                CC^[AT]GG
              198  CCAGG               
BstUI          69  CGCG                 CG^CG
               71  CGCG                
CfoI           68  GCGC                 GCG^C
               70  GCGC                
DraII         200  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              346  AGGACCT             
EcoO109I      200  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              346  AGGACCT             
EcoRII        129  CCAGG                ^CC[AT]GG
              198  CCAGG               
EcoT14I       134  CCTTGG               C^C[AT][AT]GG
              204  CCTTGG              
HaeIII          3  GGCC                 GG^CC
              132  GGCC                
HhaI           68  GCGC                 GCG^C
               70  GCGC                
HinP1I         68  GCGC                 G^CGC
               70  GCGC                
HphI          161  TCACC                GGTGA(8/7)
              175  TCACC               
MboII         288  GAAGA                GAAGA(8/7)
              405  GAAGA               
MnlI           28  GAGG                 CCTC(7/6)
              345  GAGG                
MvaI          129  CCAGG                CC^[AT]GG
              198  CCAGG               
MvnI           69  CGCG                 CG^CG
               71  CGCG                
MwoI          124  GCCACCCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              299  GCATCCTGTGC         
NlaIII        230  CATG                 CATG^
              257  CATG                
PpuMI         200  AGGTCCT              [GA]G^G[AT]CC[CT]
              346  AGGACCT             
ScrFI         129  CCAGG                CC^[ACGT]GG
              198  CCAGG               
SfaNI         299  GCATC                GCATC(5/9)
              312  GCATC               
StyI          134  CCTTGG               C^C[AT][AT]GG
              204  CCTTGG              
TspRI         180  CAGTG                CA[GC]TG[ACGT][ACGT]^
              278  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AvaII       BsaJI       BsiYI       BslI        
Cac8I       Cfr13I      DdeI        Fnu4HI      ItaI        
Sau96I      
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