Restriction map for pF1KB7429
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BcgI        BclI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     ClaI        CpoI        DpnII       DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        HphI        KpnI        MboI        MfeI        
MflI        MluI        MroI        MunI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tsp509I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI          586  GTAC                 GT^AC
AlwNI         191  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       770  AGCGCT               AGC^GCT
AspHI         207  GAGCAC               G[AT]GC[AT]^C
AspI          880  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          641  TGGCCA               TGG^CCA
BciVI         865  GGATAC               GTATCC(6/5)
BlpI          105  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      105  GCTTAGC              GC^T[ACGT]AGC
BsaBI         507  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          706  GAGACC               GGTCTC(1/5)
BseRI         138  GAGGAG               GAGGAG(10/8)
BsiHKAI       207  GAGCAC               G[AT]GC[AT]^C
BssHII        635  GCGCGC               G^CGCGC
BstAPI        875  GCACCGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         105  GCTTAGC              GC^T[ACGT]AGC
Eco47III      770  AGCGCT               AGC^GCT
EcoNI         152  CCTGGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI         718  GATTC                G^A[ACGT]TC
KspI          212  CCGCGG               CCGC^GG
MaeIII        310  GTGAC                ^GT[ACGT]AC
MamI          507  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         641  TGGCCA               TGG^CCA
MscI          641  TGGCCA               TGG^CCA
MseI          554  TTAA                 T^TAA
MspA1I        212  CCGCGG               C[AC]G^C[GT]G
PflFI         880  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         801  CCACTTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         482  GGGACCT              [GA]G^G[AT]CC[CT]
PstI           70  CTGCAG               CTGCA^G
RsaI          586  GTAC                 GT^AC
SacII         212  CCGCGG               CCGC^GG
SfcI           70  CTGCAG               C^T[GA][CT]AG
SmlI          649  CTCAAG               C^T[CT][GA]AG
StuI          149  AGGCCT               AGG^CCT
TfiI          718  GATTC                G^A[AT]TC
Tru9I         554  TTAA                 T^TAA
Tsp45I        310  GTGAC                ^GT[GC]AC
Tth111I       880  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        801  CCACTTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          455  CCACACGCTCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AocI           54  CCTCAGG              CC^T[ACGT]AGG
              689  CCTCAGG             
ApaI          591  GGGCCC               GGGCC^C
              621  GGGCCC              
AvaII         468  GGACC                G^G[AT]CC
              483  GGACC               
BanII         591  GGGCCC               G[GA]GC[CT]^C
              621  GGGCCC              
BfaI          172  CTAG                 C^TAG
              296  CTAG                
BpmI          229  CTGGAG               CTGGAG(16/14)
              244  CTGGAG              
BsgI          627  CTGCAC               GTGCAG(16/14)
              544  GTGCAG              
BsmAI         706  GAGAC                GTCTC(1/5)
              753  GTCTC               
BsmFI         482  GGGAC                GGGAC(10/14)
              698  GGGAC               
BsrI          588  ACTGG                ACTGG(1/-1)
              495  CCAGT               
BssSI         274  CTCGTG               CACGAG(-5/-1)
              670  CTCGTG              
Bsu36I         54  CCTCAGG              CC^T[ACGT]AGG
              689  CCTCAGG             
DraIII        492  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              802  CACTTCGTG           
DsaI          212  CCGCGG               C^C[GA][CT]GG
              302  CCGTGG              
EarI           40  GAAGAG               CTCTTC(1/4)
               95  GAAGAG              
Eco81I         54  CCTCAGG              CC^T[ACGT]AGG
              689  CCTCAGG             
EcoT14I        62  CCTTGG               C^C[AT][AT]GG
              314  CCAAGG              
HgaI          700  GACGC                GACGC(5/10)
              884  GCGTC               
Ksp632I        40  GAAGAG               CTCTTC(1/4)
               95  GAAGAG              
MaeI          172  CTAG                 C^TAG
              296  CTAG                
MaeII         743  ACGT                 A^CGT
              833  ACGT                
MslI          292  CATGCTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              537  CACTGAGGTG          
NaeI           13  GCCGGC               GCC^GGC
              811  GCCGGC              
NgoMIV         13  GCCGGC               G^CCGGC
              811  GCCGGC              
SapI           40  GAAGAGC              GCTCTTC(1/4)
               95  GAAGAGC             
SfaNI         580  GATGC                GCATC(5/9)
              477  GCATC               
StyI           62  CCTTGG               C^C[AT][AT]GG
              314  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        BanI        
BbeI        BbvI        BcnI        BmyI        BsaHI       
BsaJI       BsiYI       BslI        Bsp1286I    BspMI       
BsrBI       BsrFI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
ItaI        KasI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NlaIII      NlaIV       Sau96I      ScrFI       SfoI        
TseI        TspRI       
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