Restriction map for pF1KB7428
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BciVI       BclI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BseAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EcoNI       EcoRV       
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        XbaI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1129  GTCTAC               GT^[AC][GT]AC
AcyI          393  GACGCC               G[GA]^CG[CT]C
Aor51HI       615  AGCGCT               AGC^GCT
AspI          774  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          939  GGCACC               G^G[CT][GA]CC
BglI          763  GCCCCTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          637  CTCCAG               CTGGAG(16/14)
BsaHI         393  GACGCC               G[GA]^CG[CT]C
BsaWI         104  ACCGGA               [AT]^CCGG[AT]
BsgI         1193  CTGCAC               GTGCAG(16/14)
BsiEI         551  CGGCCG               CG[GA][CT]^CG
BsiWI         596  CGTACG               C^GTACG
BsmAI         607  GAGAC                GTCTC(1/5)
BspMI         426  GCAGGT               ACCTGC(4/8)
BssSI         286  CACGAG               CACGAG(-5/-1)
BstEII        459  GGTGACC              G^GT[ACGT]ACC
BstPI         459  GGTGACC              G^GT[ACGT]ACC
BstXI         264  CCAGCCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII        1116  GATC                 ^GATC
DsaI         1026  CCGTGG               C^C[GA][CT]GG
EaeI          551  CGGCCG               [CT]^GGCC[GA]
EagI          551  CGGCCG               C^GGCCG
EclXI         551  CGGCCG               C^GGCCG
Eco47III      615  AGCGCT               AGC^GCT
Eco52I        551  CGGCCG               C^GGCCG
EcoO65I       459  GGTGACC              G^GT[ACGT]ACC
EcoRI         875  GAATTC               G^AATTC
EcoT22I       827  ATGCAT               ATGCA^T
HgaI          393  GACGC                GACGC(5/10)
Hin1I         393  GACGCC               G[GA]^CG[CT]C
MboI         1116  GATC                 ^GATC
NdeII        1116  GATC                 ^GATC
NsiI          827  ATGCAT               ATGCA^T
PaeR7I        611  CTCGAG               C^TCGAG
PflFI         774  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          39  CCAGGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           10  CTGCAG               CTGCA^G
Sau3AI       1116  GATC                 ^GATC
SmlI          611  CTCGAG               C^T[CT][GA]AG
Tth111I       774  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         39  CCAGGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          611  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          352  GAATTT               [GA]^AATT[CT]
              875  GAATTC              
AocI          121  CCTGAGG              CC^T[ACGT]AGG
             1041  CCTGAGG             
ApoI          352  GAATTT               [GA]^AATT[CT]
              875  GAATTC              
AvaI          611  CTCGAG               C^[CT]CG[GA]G
              725  CTCGGG              
BcgI          422  CGAAGCAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               21  GCAGGCAGCTCG        
BcnI          197  CCGGG                CC^[GC]GG
              754  CCCGG               
BfaI          260  CTAG                 C^TAG
             1111  CTAG                
BlpI          618  GCTCAGC              GC^T[ACGT]AGC
              835  GCTCAGC             
Bpu1102I      618  GCTCAGC              GC^T[ACGT]AGC
              835  GCTCAGC             
BsoBI         611  CTCGAG               C^[CT]CG[GA]G
              725  CTCGGG              
BsrBI         697  CCGCTC               CCGCTC(-3/-3)
             1051  GAGCGG              
Bsu36I        121  CCTGAGG              CC^T[ACGT]AGG
             1041  CCTGAGG             
CelII         618  GCTCAGC              GC^T[ACGT]AGC
              835  GCTCAGC             
Eco81I        121  CCTGAGG              CC^T[ACGT]AGG
             1041  CCTGAGG             
HaeII         615  AGCGCT               [GA]GCGC^[CT]
              730  GGCGCT              
HphI          459  GGTGA                GGTGA(8/7)
              545  TCACC               
MaeI          260  CTAG                 C^TAG
             1111  CTAG                
MaeIII        460  GTGAC                ^GT[ACGT]AC
              514  GTCAC               
MseI          857  TTAA                 T^TAA
             1070  TTAA                
NciI          197  CCGGG                CC^[GC]GG
              754  CCCGG               
SacI          325  GAGCTC               GAGCT^C
              634  GAGCTC              
SapI         1048  GAAGAGC              GCTCTTC(1/4)
              699  GCTCTTC             
SfcI           10  CTGCAG               C^T[GA][CT]AG
              112  CTACAG              
Tru9I         857  TTAA                 T^TAA
             1070  TTAA                
Tsp45I        460  GTGAC                ^GT[GC]AC
              514  GTCAC               
XcmI          467  CCAAGGGCGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              777  CCAGTCCAGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
AspHI       AvaII       BanII       BbvCI       BbvI        
BmyI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NlaIII      NlaIV       NspI        PleI        PpuMI       
PvuII       RsaI        Sau96I      ScrFI       SfaNI       
SphI        StyI        TaqI        TseI        Tsp509I     
TspRI       TthHB8I     
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