Restriction map for pF1KB7428
 (Circular) (Six-base) MAPSORT of: pF1KB7428.seq  Check: 3656  from: 1  to: 4349



pF1KB7428  4349 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    525     525 
   Size:       4349
 
AccI GT'mk_AC

Cuts at:   1238    1387    1238 
   Size:        149    4200
 
Acc65I G'GTAC_C

Cuts at:   1365    1365 
   Size:       4349
 
AclI AA'CG_TT

Cuts at:   4189    4189 
   Size:       4349
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    296    2174    2606    3353    3621     296 
   Size:       1878     432     747     268    1024

  Fragments arranged by size: 

                1878    1024     747     432     268
 
AfeI AGC'GCT

Cuts at:    725     725 
   Size:       4349
 
AflIII A'CryG_T

Cuts at:   1701    2805    3782    1701 
   Size:       1104     977    2268

  Fragments arranged by size: 

                2268    1104     977
 
AgeI A'CCGG_T

Cuts at:   2737    2737 
   Size:       4349
 
AlwNI CAG_nnn'CTG

Cuts at:   1654    3221    1654 
   Size:       1567    2782
 
ApaI G_GGCC'C

Cuts at:    348     355    1325     348 
   Size:          7     970    3372

  Fragments arranged by size: 

                3372     970       7
 
ApaLI G'TGCA_C

Cuts at:   3119    3119 
   Size:       4349
 
ApoI r'AATT_y

Cuts at:    460     983    1353    3702    3958     460 
   Size:        523     370    2349     256     851

  Fragments arranged by size: 

                2349     851     523     370     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4349
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4349
 
AvaI C'yCGr_G

Cuts at:    719     833    1369     719 
   Size:        114     536    3699

  Fragments arranged by size: 

                3699     536     114
 
BamHI G'GATC_C

Cuts at:   1374    1374 
   Size:       4349
 
BanI G'GyrC_C

Cuts at:   1047    1365    1779    2027    2062    1047 
   Size:        318     414     248      35    3334

  Fragments arranged by size: 

                3334     414     318     248      35
 
BanII G_rGCy'C

Cuts at:    348     355     437     746    1061    1325    1363    2393     348 
   Size:          7      82     309     315     264      38    1030    2304

  Fragments arranged by size: 

                2304    1030     315     309     264      82      38       7
            
 
BbeI G_GCGC'C

Cuts at:   1783    2031    1783 
   Size:        248    4101
 
BbvCI CC'TCA_GC

Cuts at:    257     326     634     257 
   Size:         69     308    3972

  Fragments arranged by size: 

                3972     308      69
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    118     152     519     553     118 
   Size:         34     367      34    3914

  Fragments arranged by size: 

                3914     367      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2247    3008    4349    2247 
   Size:        761    1341    2247

  Fragments arranged by size: 

                2247    1341     761
 
BclI T'GATC_A

Cuts at:   1869    1869 
   Size:       4349
 
BfrBI ATG'CAT

Cuts at:    937     937 
   Size:       4349
 
BglI GCCn_nnn'nGGC

Cuts at:    112     877     112 
   Size:        765    3584
 
BglII A'GATC_T

Cuts at:   1864    3901    1864 
   Size:       2037    2312
 
BlpI GC'TnA_GC

Cuts at:    727     944    1461     727 
   Size:        217     517    3615

  Fragments arranged by size: 

                3615     517     217
 
Bme1580I G_kGCm'C

Cuts at:    348     355     521    1325    1974    2067    3123     348 
   Size:          7     166     804     649      93    1056    1574

  Fragments arranged by size: 

                1574    1056     804     649     166      93       7
 
BmrI ACTGGGnnnn_n'

Cuts at:    675     879    1033    1115    1561    1642    1977    4121     675 
   Size:        204     154      82     446      81     335    2144     903

  Fragments arranged by size: 

                2144     903     446     335     204     154      82      81
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2716    2716 
   Size:       4349
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    730     730 
   Size:       4349
 
Bpu10I CC'TnA_GC

Cuts at:    257     326     634     842     257 
   Size:         69     308     208    3764

  Fragments arranged by size: 

                3764     308     208      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1525    2404    2896    3194    1525 
   Size:        879     492     298    2680

  Fragments arranged by size: 

                2680     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4053    4053 
   Size:       4349
 
BsaAI yAC'GTr

Cuts at:   1702    1702 
   Size:       4349
 
BsaBI GATnn'nnATC

Cuts at:      6    1863    3900       6 
   Size:       1857    2037     455

  Fragments arranged by size: 

                2037    1857     455
 
BsaHI Gr'CG_yC

Cuts at:    502    1780    2028    2730    4226     502 
   Size:       1278     248     702    1496     625

  Fragments arranged by size: 

                1496    1278     702     625     248
 
BsaWI w'CCGG_w

Cuts at:    212    1538    2059    2737    3011    3158    3569     212 
   Size:       1326     521     678     274     147     411     992

  Fragments arranged by size: 

                1326     992     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    786     816     786 
   Size:         30    4319
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    301     328     331     343     988    1069     301 
   Size:         27       3      12     645      81    3581

  Fragments arranged by size: 

                3581     645      81      27      12       3
 
BseYI C'CCAG_C

Cuts at:    355     849     900    1774    2532    3109    3605     355 
   Size:        494      51     874     758     577     496    1099

  Fragments arranged by size: 

                1099     874     758     577     496     494      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1286    1286 
   Size:       4349
 
BsiEI CG_ry'CG

Cuts at:    104     662    1891    1937    3145     104 
   Size:        558    1229      46    1208    1308

  Fragments arranged by size: 

                1308    1229    1208     558      46
 
BsiHKAI G_wGCw'C

Cuts at:    437     542     746    1363    2141    2331    3123     437 
   Size:        105     204     617     778     190     792    1663

  Fragments arranged by size: 

                1663     792     778     617     204     190     105
 
BsiWI C'GTAC_G

Cuts at:    704     704 
   Size:       4349
 
Bsp1286I G_dGCh'C

Cuts at:    348     355     437     521     542     746    1061    1325    1363 
   Size:          7      82      84      21     204     315     264      38

Cuts at:   1363    1974    2067    2141    2331    2393    3123     348 
   Size:        611      93      74     190      62     730    1574

  Fragments arranged by size: 

                1574     730     611     315     264     204     190      93
                  84      82      74      62      38      21       7
 
BspEI T'CCGG_A

Cuts at:   3569    3569 
   Size:       4349
 
BspHI T'CATG_A

Cuts at:   3525    3525 
   Size:       4349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    525    1399    1613    1915    2296     525 
   Size:        874     214     302     381    2578

  Fragments arranged by size: 

                2578     874     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     807    1161    2641    2695    4179    4344      50 
   Size:        757     354    1480      54    1484     165      55

  Fragments arranged by size: 

                1484    1480     757     354     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2261    2261 
   Size:       4349
 
BsrFI r'CCGG_y

Cuts at:   2347    2528    2737    2347 
   Size:        181     209    3959

  Fragments arranged by size: 

                3959     209     181
 
BssSI C'ACGA_G

Cuts at:    394    2620    2978     394 
   Size:       2226     358    1765

  Fragments arranged by size: 

                2226    1765     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1726    1726 
   Size:       4349
 
BstBI TT'CG_AA

Cuts at:   2710    2710 
   Size:       4349
 
BstEII G'GTnAC_C

Cuts at:    567     567 
   Size:       4349
 
BstXI CCAn_nnnn'nTGG

Cuts at:    379     379 
   Size:       4349
 
BstYI r'GATC_y

Cuts at:      1    1374    1841    1864    2199    2445    3457    3543    3555 
   Size:       1373     467      23     335     246    1012      86      12

Cuts at:   3555    3901       1 
   Size:        346     449

  Fragments arranged by size: 

                1373    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    230    1150     230 
   Size:        920    3429
 
BtgI C'CryG_G

Cuts at:    107    1134     107 
   Size:       1027    3322
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2299    2480    2643    2299 
   Size:        181     163    4005

  Fragments arranged by size: 

                4005     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1003    1197    1592    1003 
   Size:        194     395    3760

  Fragments arranged by size: 

                3760     395     194
 
DraI TTT'AAA

Cuts at:   1348    1348 
   Size:       4349
 
DrdI GACnn_nn'nnGTC

Cuts at:   2055    2913    2055 
   Size:        858    3491
 
EaeI y'GGCC_r

Cuts at:    659    1934    2108    2499    2526    3871     659 
   Size:       1275     174     391      27    1345    1137

  Fragments arranged by size: 

                1345    1275    1137     391     174      27
 
EagI C'GGCC_G

Cuts at:    659    1934     659 
   Size:       1275    3074
 
EarI CTCTTCn'nnn_

Cuts at:    257     814    1063    1151    2372    2582     257 
   Size:        557     249      88    1221     210    2024

  Fragments arranged by size: 

                2024    1221     557     249     210      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2557    2867    3013    4159    2557 
   Size:        310     146    1146    2747

  Fragments arranged by size: 

                2747    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    435     744    1361     435 
   Size:        309     617    3423

  Fragments arranged by size: 

                3423     617     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    296     730    2174    2606    3353    3621     296 
   Size:        434    1444     432     747     268    1024

  Fragments arranged by size: 

                1444    1024     747     434     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    114     225     236     344     351     352     846     859    1027 
   Size:        111      11     108       7       1     494      13     168

Cuts at:   1027    1087    1321    1322    1488     114 
   Size:         60     234       1     166    2975

  Fragments arranged by size: 

                2975     494     234     168     166     111     108      60
                  13      11       7       1       1
 
EcoRI G'AATT_C

Cuts at:    983    1353     983 
   Size:        370    3979
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2368    2368 
   Size:       4349
 
FspI TGC'GCA

Cuts at:   2130    2130 
   Size:       4349
 
HaeII r_GCGC'y

Cuts at:    727     842    1783    2031    3053    4032     727 
   Size:        115     941     248    1022     979    1044

  Fragments arranged by size: 

                1044    1022     979     941     248     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2191    2221    2223    2253    2191 
   Size:         30       2      30    4287

  Fragments arranged by size: 

                4287      30      30       2
 
HincII GTy'rAC

Cuts at:   1388    1388 
   Size:       4349
 
KasI G'GCGC_C

Cuts at:   1779    2027    1779 
   Size:        248    4101
 
KpnI G_GTAC'C

Cuts at:   1369    1369 
   Size:       4349
 
MluI A'CGCG_T

Cuts at:   3782    3782 
   Size:       4349
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1315    3020    3204    1315 
   Size:       1705     184    2460

  Fragments arranged by size: 

                2460    1705     184
 
MscI TGG'CCA

Cuts at:   2110    2110 
   Size:       4349
 
MslI CAynn'nnrTG

Cuts at:   2338    2465    2338 
   Size:        127    4222
 
MspA1I CmG'CkG

Cuts at:    180     325     426     948    1460    1774    2134    3147    3392 
   Size:        145     101     522     512     314     360    1013     245

Cuts at:   3392     180 
   Size:       1137

  Fragments arranged by size: 

                1137    1013     522     512     360     314     245     145
                 101
 
NaeI GCC'GGC

Cuts at:   2530    2530 
   Size:       4349
 
NarI GG'CG_CC

Cuts at:   1780    2028    1780 
   Size:        248    4101
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4349
 
NgoMIV G'CCGG_C

Cuts at:   2528    2528 
   Size:       4349
 
NsiI A_TGCA'T

Cuts at:    939     939 
   Size:       4349
 
NspI r_CATG'y

Cuts at:    176     937     941    1116    1402    1902    2809     176 
   Size:        761       4     175     286     500     907    1716

  Fragments arranged by size: 

                1716     907     761     500     286     175       4
 
PasI CC'CwG_GG

Cuts at:    147     348     736     906    1284     147 
   Size:        201     388     170     378    3212

  Fragments arranged by size: 

                3212     388     378     201     170
 
PciI A'CATG_T

Cuts at:   2805    2805 
   Size:       4349
 
PflMI CCAn_nnn'nTGG

Cuts at:    153     153 
   Size:       4349
 
PmeI GTTT'AAAC

Cuts at:   1348    1348 
   Size:       4349
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3488    3520    3488 
   Size:         32    4317
 
PpuMI rG'GwC_Cy

Cuts at:    846     859    1027     846 
   Size:         13     168    4168

  Fragments arranged by size: 

                4168     168      13
 
PspOMI G'GGCC_C

Cuts at:    344     351    1321     344 
   Size:          7     970    3372

  Fragments arranged by size: 

                3372     970       7
 
PspXI vC'TCGA_Gb

Cuts at:    719     719 
   Size:       4349
 
PstI C_TGCA'G

Cuts at:    122    1396    2081     122 
   Size:       1274     685    2390

  Fragments arranged by size: 

                2390    1274     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4349
 
PvuII CAG'CTG

Cuts at:    325     426     948    1774    2134     325 
   Size:        101     522     826     360    2540

  Fragments arranged by size: 

                2540     826     522     360     101
 
RsrII CG'GwC_CG

Cuts at:   2544    2544 
   Size:       4349
 
SacI G_AGCT'C

Cuts at:    437     746    1363     437 
   Size:        309     617    3423

  Fragments arranged by size: 

                3423     617     309
 
SalI G'TCGA_C

Cuts at:   1386    1386 
   Size:       4349
 
SanDI GG'GwC_CC

Cuts at:    859     859 
   Size:       4349
 
SapI GCTCTTCn'nnn_

Cuts at:    814    1151    2372    2582     814 
   Size:        337    1221     210    2581

  Fragments arranged by size: 

                2581    1221     337     210
 
SbfI CC_TGCA'GG

Cuts at:   1396    1396 
   Size:       4349
 
ScaI AGT'ACT

Cuts at:   3866    3866 
   Size:       4349
 
SfcI C'TryA_G

Cuts at:     33     118     220    1392    2077    3070    3261      33 
   Size:         85     102    1172     685     993     191    1121

  Fragments arranged by size: 

                1172    1121     993     685     191     102      85
 
SfoI GGC'GCC

Cuts at:   1781    2029    1781 
   Size:        248    4101
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4349
 
SmaI CCC'GGG

Cuts at:   1371    1371 
   Size:       4349
 
SmlI C'TyrA_G

Cuts at:    719    1504    2419    2911    3173     719 
   Size:        785     915     492     262    1895

  Fragments arranged by size: 

                1895     915     785     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4349
 
SphI G_CATG'C

Cuts at:    176     937     941    1116    1402    1902     176 
   Size:        761       4     175     286     500    2623

  Fragments arranged by size: 

                2623     761     500     286     175       4
 
StyI C'CwwG_G

Cuts at:    107     239     575     854    1483    1837     107 
   Size:        132     336     279     629     354    2619

  Fragments arranged by size: 

                2619     629     354     336     279     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    694    1932    2065    2524    2733     694 
   Size:       1238     133     459     209    2310

  Fragments arranged by size: 

                2310    1238     459     209     133
 
TatI w'GTAC_w

Cuts at:    683    3864     683 
   Size:       3181    1168
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    786     818    3866    3898     786 
   Size:         32    3048      32    1237

  Fragments arranged by size: 

                3048    1237      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    885    2146     885 
   Size:       1261    3088
 
XbaI T'CTAG_A

Cuts at:   1380    1380 
   Size:       4349
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    582     892    1844     582 
   Size:        310     952    3087

  Fragments arranged by size: 

                3087     952     310
 
XhoI C'TCGA_G

Cuts at:    719     719 
   Size:       4349
 
XmaI C'CCGG_G

Cuts at:   1369    1369 
   Size:       4349
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AleI     AlfI     AloI     AscI    AvrII
     BaeI     BbsI    BmgBI     BmtI     BsmI    BsmBI    BsrGI   BssHII
  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI
     PfoI     PmlI    PshAI     PsiI     PsrI    SacII    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com