Restriction map for pF1KB7428
 (Linear) (Six-base) MAPSORT of: KIBB7428.seq  Check: 5315  from: 1  to: 1236



KIBB7428.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     417    1236 
   Size:        417     819
 
AccI GT'mk_AC

Cuts at:      0    1130    1236 
   Size:       1130     106
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188    1236 
   Size:        188    1048
 
AfeI AGC'GCT

Cuts at:      0     617    1236 
   Size:        617     619
 
ApaI G_GGCC'C

Cuts at:      0     240     247    1217    1236 
   Size:        240       7     970      19

  Fragments arranged by size: 

                 970     240      19       7
 
ApoI r'AATT_y

Cuts at:      0     352     875    1236 
   Size:        352     523     361

  Fragments arranged by size: 

                 523     361     352
 
AvaI C'yCGr_G

Cuts at:      0     611     725    1236 
   Size:        611     114     511

  Fragments arranged by size: 

                 611     511     114
 
BanI G'GyrC_C

Cuts at:      0     939    1236 
   Size:        939     297
 
BanII G_rGCy'C

Cuts at:      0     240     247     329     638     953    1217    1236 
   Size:        240       7      82     309     315     264      19

  Fragments arranged by size: 

                 315     309     264     240      82      19       7
 
BbvCI CC'TCA_GC

Cuts at:      0     149     218     526    1236 
   Size:        149      69     308     710

  Fragments arranged by size: 

                 710     308     149      69
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      10      44     411     445    1236 
   Size:         10      34     367      34     791

  Fragments arranged by size: 

                 791     367      34      34      10
 
BfrBI ATG'CAT

Cuts at:      0     829    1236 
   Size:        829     407
 
BglI GCCn_nnn'nGGC

Cuts at:      0     769    1236 
   Size:        769     467
 
BlpI GC'TnA_GC

Cuts at:      0     619     836    1236 
   Size:        619     217     400

  Fragments arranged by size: 

                 619     400     217
 
Bme1580I G_kGCm'C

Cuts at:      0     240     247     413    1217    1236 
   Size:        240       7     166     804      19

  Fragments arranged by size: 

                 804     240     166      19       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     567     771     925    1007    1236 
   Size:        567     204     154      82     229

  Fragments arranged by size: 

                 567     229     204     154      82
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622    1236 
   Size:        622     614
 
Bpu10I CC'TnA_GC

Cuts at:      0     149     218     526     734    1236 
   Size:        149      69     308     208     502

  Fragments arranged by size: 

                 502     308     208     149      69
 
BsaHI Gr'CG_yC

Cuts at:      0     394    1236 
   Size:        394     842
 
BsaWI w'CCGG_w

Cuts at:      0     104    1236 
   Size:        104    1132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     678     708    1236 
   Size:        678      30     528

  Fragments arranged by size: 

                 678     528      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     193     220     223     235     880     961    1236 
   Size:        193      27       3      12     645      81     275

  Fragments arranged by size: 

                 645     275     193      81      27      12       3
 
BseYI C'CCAG_C

Cuts at:      0     247     741     792    1236 
   Size:        247     494      51     444

  Fragments arranged by size: 

                 494     444     247      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1178    1236 
   Size:       1178      58
 
BsiEI CG_ry'CG

Cuts at:      0     554    1236 
   Size:        554     682
 
BsiHKAI G_wGCw'C

Cuts at:      0     329     434     638    1236 
   Size:        329     105     204     598

  Fragments arranged by size: 

                 598     329     204     105
 
BsiWI C'GTAC_G

Cuts at:      0     596    1236 
   Size:        596     640
 
Bsp1286I G_dGCh'C

Cuts at:      0     240     247     329     413     434     638     953    1217 
   Size:        240       7      82      84      21     204     315     264

Cuts at:   1217    1236 
   Size:         19

  Fragments arranged by size: 

                 315     264     240     204      84      82      21      19
                   7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     417    1236 
   Size:        417     819
 
BsrBI CCG'CTC

Cuts at:      0     699    1053    1236 
   Size:        699     354     183

  Fragments arranged by size: 

                 699     354     183
 
BssSI C'ACGA_G

Cuts at:      0     286    1236 
   Size:        286     950
 
BstEII G'GTnAC_C

Cuts at:      0     459    1236 
   Size:        459     777
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     271    1236 
   Size:        271     965
 
Bsu36I CC'TnA_GG

Cuts at:      0     122    1042    1236 
   Size:        122     920     194

  Fragments arranged by size: 

                 920     194     122
 
BtgI C'CryG_G

Cuts at:      0    1026    1236 
   Size:       1026     210
 
BtsI GCAGTG_nn'

Cuts at:      0     895    1089    1236 
   Size:        895     194     147

  Fragments arranged by size: 

                 895     194     147
 
EaeI y'GGCC_r

Cuts at:      0     551    1236 
   Size:        551     685
 
EagI C'GGCC_G

Cuts at:      0     551    1236 
   Size:        551     685
 
EarI CTCTTCn'nnn_

Cuts at:      0     149     706     955    1043    1236 
   Size:        149     557     249      88     193

  Fragments arranged by size: 

                 557     249     193     149      88
 
EcoICRI GAG'CTC

Cuts at:      0     327     636    1236 
   Size:        327     309     600

  Fragments arranged by size: 

                 600     327     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     622    1236 
   Size:        188     434     614

  Fragments arranged by size: 

                 614     434     188
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     117     128     236     243     244     738     751 
   Size:          6     111      11     108       7       1     494      13

Cuts at:    751     919     979    1213    1214    1236 
   Size:        168      60     234       1      22

  Fragments arranged by size: 

                 494     234     168     111     108      60      22      13
                  11       7       6       1       1
 
EcoRI G'AATT_C

Cuts at:      0     875    1236 
   Size:        875     361
 
HaeII r_GCGC'y

Cuts at:      0     619     734    1236 
   Size:        619     115     502

  Fragments arranged by size: 

                 619     502     115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1207    1236 
   Size:       1207      29
 
MspA1I CmG'CkG

Cuts at:      0      72     217     318     840    1236 
   Size:         72     145     101     522     396

  Fragments arranged by size: 

                 522     396     145     101      72
 
NsiI A_TGCA'T

Cuts at:      0     831    1236 
   Size:        831     405
 
NspI r_CATG'y

Cuts at:      0      68     829     833    1008    1236 
   Size:         68     761       4     175     228

  Fragments arranged by size: 

                 761     228     175      68       4
 
PasI CC'CwG_GG

Cuts at:      0      39     240     628     798    1176    1236 
   Size:         39     201     388     170     378      60

  Fragments arranged by size: 

                 388     378     201     170      60      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      45    1236 
   Size:         45    1191
 
PpuMI rG'GwC_Cy

Cuts at:      0     738     751     919    1236 
   Size:        738      13     168     317

  Fragments arranged by size: 

                 738     317     168      13
 
PspOMI G'GGCC_C

Cuts at:      0     236     243    1213    1236 
   Size:        236       7     970      23

  Fragments arranged by size: 

                 970     236      23       7
 
PspXI vC'TCGA_Gb

Cuts at:      0     611    1236 
   Size:        611     625
 
PstI C_TGCA'G

Cuts at:      0      14    1236 
   Size:         14    1222
 
PvuII CAG'CTG

Cuts at:      0     217     318     840    1236 
   Size:        217     101     522     396

  Fragments arranged by size: 

                 522     396     217     101
 
SacI G_AGCT'C

Cuts at:      0     329     638    1236 
   Size:        329     309     598

  Fragments arranged by size: 

                 598     329     309
 
SanDI GG'GwC_CC

Cuts at:      0     751    1236 
   Size:        751     485
 
SapI GCTCTTCn'nnn_

Cuts at:      0     706    1043    1236 
   Size:        706     337     193

  Fragments arranged by size: 

                 706     337     193
 
SfcI C'TryA_G

Cuts at:      0      10     112    1236 
   Size:         10     102    1124

  Fragments arranged by size: 

                1124     102      10
 
SmlI C'TyrA_G

Cuts at:      0     611    1236 
   Size:        611     625
 
SphI G_CATG'C

Cuts at:      0      68     829     833    1008    1236 
   Size:         68     761       4     175     228

  Fragments arranged by size: 

                 761     228     175      68       4
 
StyI C'CwwG_G

Cuts at:      0     131     467     746    1236 
   Size:        131     336     279     490

  Fragments arranged by size: 

                 490     336     279     131
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     586    1236 
   Size:        586     650
 
TatI w'GTAC_w

Cuts at:      0     575    1236 
   Size:        575     661
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     678     710    1236 
   Size:        678      32     526

  Fragments arranged by size: 

                 678     526      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     777    1236 
   Size:        777     459
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     474     784    1236 
   Size:        474     310     452

  Fragments arranged by size: 

                 474     452     310
 
XhoI C'TCGA_G

Cuts at:      0     611    1236 
   Size:        611     625
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     ApaI     ApoI     AvaI     BanI
    BanII    BbvCI     BcgI    BfrBI     BglI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspMI    BsrBI    BssSI   BstEII
    BstXI   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI    HaeII     MmeI   MspA1I     NsiI     NspI
     PasI    PflMI    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI
    SanDI     SapI     SfcI     SmlI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BciVI     BclI    BglII    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EciI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     XbaI     XmaI     XmnI     ZraI
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