Restriction map for pF1KB7426
 (Linear) (Six-base) MAPSORT of: KIBB7426.seq  Check: 9681  from: 1  to: 1236



KIBB7426.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AfeI AGC'GCT

Cuts at:      0     406    1215    1236 
   Size:        406     809      21

  Fragments arranged by size: 

                 809     406      21
 
AlwNI CAG_nnn'CTG

Cuts at:      0      11    1236 
   Size:         11    1225
 
ApaI G_GGCC'C

Cuts at:      0     665    1236 
   Size:        665     571
 
AvaI C'yCGr_G

Cuts at:      0      31     265     564     664     858    1236 
   Size:         31     234     299     100     194     378

  Fragments arranged by size: 

                 378     299     234     194     100      31
 
BanI G'GyrC_C

Cuts at:      0     114     455     577     636    1236 
   Size:        114     341     122      59     600

  Fragments arranged by size: 

                 600     341     122     114      59
 
BanII G_rGCy'C

Cuts at:      0     665     715     806    1236 
   Size:        665      50      91     430

  Fragments arranged by size: 

                 665     430      91      50
 
BbeI G_GCGC'C

Cuts at:      0     459     640    1236 
   Size:        459     181     596

  Fragments arranged by size: 

                 596     459     181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1231    1236 
   Size:       1231       5
 
BbvCI CC'TCA_GC

Cuts at:      0     787    1236 
   Size:        787     449
 
BglI GCCn_nnn'nGGC

Cuts at:      0      54     575     624     660    1236 
   Size:         54     521      49      36     576

  Fragments arranged by size: 

                 576     521      54      49      36
 
BlpI GC'TnA_GC

Cuts at:      0     394    1236 
   Size:        394     842
 
Bme1580I G_kGCm'C

Cuts at:      0     665    1236 
   Size:        665     571
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1202    1236 
   Size:       1202      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     787    1236 
   Size:        787     449
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     661    1236 
   Size:        661     575
 
BsaAI yAC'GTr

Cuts at:      0     348    1236 
   Size:        348     888
 
BsaBI GATnn'nnATC

Cuts at:      0     184    1236 
   Size:        184    1052
 
BsaHI Gr'CG_yC

Cuts at:      0     456     533     637    1236 
   Size:        456      77     104     599

  Fragments arranged by size: 

                 599     456     104      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67    1178    1208    1236 
   Size:         37      30    1111      30      28

  Fragments arranged by size: 

                1111      37      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     585    1199    1236 
   Size:        585     614      37

  Fragments arranged by size: 

                 614     585      37
 
BseYI C'CCAG_C

Cuts at:      0     723    1236 
   Size:        723     513
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     364    1236 
   Size:        345      19     872

  Fragments arranged by size: 

                 872     345      19
 
BsiEI CG_ry'CG

Cuts at:      0     709     838     854    1236 
   Size:        709     129      16     382

  Fragments arranged by size: 

                 709     382     129      16
 
BsiHKAI G_wGCw'C

Cuts at:      0     485    1236 
   Size:        485     751
 
BsmI GAATG_Cn'

Cuts at:      0     706    1236 
   Size:        706     530
 
Bsp1286I G_dGCh'C

Cuts at:      0     485     665     715     806    1236 
   Size:        485     180      50      91     430

  Fragments arranged by size: 

                 485     430     180      91      50
 
BsrBI CCG'CTC

Cuts at:      0     161     429    1090    1236 
   Size:        161     268     661     146

  Fragments arranged by size: 

                 661     268     161     146
 
BsrFI r'CCGG_y

Cuts at:      0     117     508     704     830     878    1236 
   Size:        117     391     196     126      48     358

  Fragments arranged by size: 

                 391     358     196     126     117      48
 
BssHII G'CGCG_C

Cuts at:      0     467     728    1236 
   Size:        467     261     508

  Fragments arranged by size: 

                 508     467     261
 
BstEII G'GTnAC_C

Cuts at:      0     120     171     447     750    1236 
   Size:        120      51     276     303     486

  Fragments arranged by size: 

                 486     303     276     120      51
 
BtgI C'CryG_G

Cuts at:      0      52     491     671     680    1236 
   Size:         52     439     180       9     556

  Fragments arranged by size: 

                 556     439     180      52       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     459    1236 
   Size:        459     777
 
BtsI GCAGTG_nn'

Cuts at:      0    1154    1236 
   Size:       1154      82
 
DraI TTT'AAA

Cuts at:      0    1101    1236 
   Size:       1101     135
 
EaeI y'GGCC_r

Cuts at:      0     239     413     706     835     851    1236 
   Size:        239     174     293     129      16     385

  Fragments arranged by size: 

                 385     293     239     174     129      16
 
EagI C'GGCC_G

Cuts at:      0     706     835     851    1236 
   Size:        706     129      16     385

  Fragments arranged by size: 

                 706     385     129      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1202    1236 
   Size:       1202      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0     543     661    1236 
   Size:        543     118     575

  Fragments arranged by size: 

                 575     543     118
 
HaeII r_GCGC'y

Cuts at:      0      84     112     195     300     408     459     499     640 
   Size:         84      28      83     105     108      51      40     141

Cuts at:    640     688    1217    1236 
   Size:         48     529      19

  Fragments arranged by size: 

                 529     141     108     105      84      83      51      48
                  40      28      19
 
KasI G'GCGC_C

Cuts at:      0     455     636    1236 
   Size:        455     181     600

  Fragments arranged by size: 

                 600     455     181
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     272    1236 
   Size:        272     964
 
MscI TGG'CCA

Cuts at:      0     415    1236 
   Size:        415     821
 
MslI CAynn'nnrTG

Cuts at:      0     241     478    1236 
   Size:        241     237     758

  Fragments arranged by size: 

                 758     241     237
 
MspA1I CmG'CkG

Cuts at:      0      86     127     324     673     682     723     840    1236 
   Size:         86      41     197     349       9      41     117     396

  Fragments arranged by size: 

                 396     349     197     117      86      41      41       9
            
 
NaeI GCC'GGC

Cuts at:      0     706     832    1236 
   Size:        706     126     404

  Fragments arranged by size: 

                 706     404     126
 
NarI GG'CG_CC

Cuts at:      0     456     637    1236 
   Size:        456     181     599

  Fragments arranged by size: 

                 599     456     181
 
NgoMIV G'CCGG_C

Cuts at:      0     704     830    1236 
   Size:        704     126     406

  Fragments arranged by size: 

                 704     406     126
 
NotI GC'GGCC_GC

Cuts at:      0     835    1236 
   Size:        835     401
 
NspI r_CATG'y

Cuts at:      0     207    1236 
   Size:        207    1029
 
PasI CC'CwG_GG

Cuts at:      0     102     308    1236 
   Size:        102     206     928

  Fragments arranged by size: 

                 928     206     102
 
PpuMI rG'GwC_Cy

Cuts at:      0     543    1236 
   Size:        543     693
 
PsiI TTA'TAA

Cuts at:      0     922    1236 
   Size:        922     314
 
PspOMI G'GGCC_C

Cuts at:      0     661    1236 
   Size:        661     575
 
PvuII CAG'CTG

Cuts at:      0     723    1236 
   Size:        723     513
 
SacII CC_GC'GG

Cuts at:      0     674     683    1236 
   Size:        674       9     553

  Fragments arranged by size: 

                 674     553       9
 
ScaI AGT'ACT

Cuts at:      0    1003    1236 
   Size:       1003     233
 
SfoI GGC'GCC

Cuts at:      0     457     638    1236 
   Size:        457     181     598

  Fragments arranged by size: 

                 598     457     181
 
SmaI CCC'GGG

Cuts at:      0     566    1236 
   Size:        566     670
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     300    1236 
   Size:        300     936
 
TatI w'GTAC_w

Cuts at:      0     989    1001    1082    1236 
   Size:        989      12      81     154

  Fragments arranged by size: 

                 989     154      81      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69    1178    1210    1236 
   Size:         37      32    1109      32      26

  Fragments arranged by size: 

                1109      37      32      32      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     225    1236 
   Size:        225    1011
 
XmaI C'CCGG_G

Cuts at:      0     564    1236 
   Size:        564     672
 

 Enzymes that do cut:

     AfeI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BglI     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BsrBI    BsrFI   BssHII   BstEII     BtgI    BtgZI
     BtsI     DraI     EaeI     EagI  Eco57MI EcoO109I    HaeII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI
     NspI     PasI    PpuMI     PsiI   PspOMI    PvuII    SacII     ScaI
     SfoI     SmaI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaWI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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