Restriction map for pF1KB7421
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AlwNI       Aor51HI     ApaI        AscI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BspDI       
BspEI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MflI        MluI        MroI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TaqI        TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          481  GAATTC               [GA]^AATT[CT]
AflIII         72  ACATGT               A^C[GA][CT]GT
Alw44I        438  GTGCAC               G^TGCAC
AocI          218  CCTCAGG              CC^T[ACGT]AGG
ApaLI         438  GTGCAC               G^TGCAC
ApoI          481  GAATTC               [GA]^AATT[CT]
AseI           64  ATTAAT               AT^TAAT
AsnI           64  ATTAAT               AT^TAAT
AspI          961  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          662  CCCGGG               C^[CT]CG[GA]G
BanI          690  GGCACC               G^G[CT][GA]CC
BbvCI         336  CCTCAGC              CCTCAGC(-5/-2)
BpmI         1043  CTGGAG               CTGGAG(16/14)
BseRI         334  CTCCTC               GAGGAG(10/8)
BsgI          716  GTGCAG               GTGCAG(16/14)
BsmAI         736  GAGAC                GTCTC(1/5)
BsmI           97  GAATGC               GAATGC(1/-1)
BsoBI         662  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      917  TGTACA               T^GTACA
BspHI         554  TCATGA               T^CATGA
BspLU11I       72  ACATGT               A^CATGT
BsrGI         917  TGTACA               T^GTACA
BsrI         1042  ACTGG                ACTGG(1/-1)
Bsu36I        218  CCTCAGG              CC^T[ACGT]AGG
Cac8I         951  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI          309  GCGC                 GCG^C
DrdI          885  GACTGTGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        218  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1061  CCTTGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         481  GAATTC               G^AATTC
EcoT14I      1061  CCTTGG               C^C[AT][AT]GG
HhaI          309  GCGC                 GCG^C
HinP1I        309  GCGC                 G^CGC
MaeIII       1039  GTGAC                ^GT[ACGT]AC
PflFI         961  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1077  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI          64  ATTAAT               AT^TAAT
RcaI          554  TCATGA               T^CATGA
SmaI          662  CCCGGG               CCC^GGG
SspBI         917  TGTACA               T^GTACA
StyI         1061  CCTTGG               C^C[AT][AT]GG
Tsp45I       1039  GTGAC                ^GT[GC]AC
Tth111I       961  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          422  CCATTTCCTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         662  CCCGGG               C^CCGGG
XmaI          662  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaII         419  GGACC                G^G[AT]CC
             1078  GGTCC               
BalI          269  TGGCCA               TGG^CCA
              521  TGGCCA              
BspMI         404  ACCTGC               ACCTGC(4/8)
              586  GCAGGT              
DraI           21  TTTAAA               TTT^AAA
               42  TTTAAA              
DraII        1057  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1077  GGGTCCT             
EaeI          269  TGGCCA               [CT]^GGCC[GA]
              521  TGGCCA              
EarI          157  GAAGAG               CTCTTC(1/4)
             1053  GAAGAG              
EcoO109I     1057  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1077  GGGTCCT             
HapII         564  CCGG                 C^CGG
              663  CCGG                
HinfI         774  GATTC                G^A[ACGT]TC
              809  GAATC               
HpaII         564  CCGG                 C^CGG
              663  CCGG                
HphI          257  TCACC                GGTGA(8/7)
              635  TCACC               
Ksp632I       157  GAAGAG               CTCTTC(1/4)
             1053  GAAGAG              
MluNI         269  TGGCCA               TGG^CCA
              521  TGGCCA              
MscI          269  TGGCCA               TGG^CCA
              521  TGGCCA              
MspI          564  CCGG                 C^CGG
              663  CCGG                
SacI          834  GAGCTC               GAGCT^C
             1046  GAGCTC              
SfaNI         390  GATGC                GCATC(5/9)
              765  GATGC               
TfiI          774  GATTC                G^A[AT]TC
              809  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      ItaI        
MboI        MboII       MnlI        MseI        MslI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        Tru9I       TseI        Tsp509I     
TspRI       
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