Restriction map for pF1KB7421
 (Linear) (Six-base) MAPSORT of: KIBB7421.seq  Check: 8216  from: 1  to: 1107



KIBB7421.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1072    1107 
   Size:        724     348      35

  Fragments arranged by size: 

                 724     348      35
 
AflIII A'CryG_T

Cuts at:      0      72    1107 
   Size:         72    1035
 
AleI CACnn'nnGTG

Cuts at:      0     379    1107 
   Size:        379     728
 
ApaLI G'TGCA_C

Cuts at:      0     438    1107 
   Size:        438     669
 
ApoI r'AATT_y

Cuts at:      0     481    1107 
   Size:        481     626
 
AseI AT'TA_AT

Cuts at:      0      65    1107 
   Size:         65    1042
 
AvaI C'yCGr_G

Cuts at:      0     662    1107 
   Size:        662     445
 
BanI G'GyrC_C

Cuts at:      0     690    1107 
   Size:        690     417
 
BanII G_rGCy'C

Cuts at:      0     607     838    1050    1107 
   Size:        607     231     212      57

  Fragments arranged by size: 

                 607     231     212      57
 
BbvCI CC'TCA_GC

Cuts at:      0     337    1107 
   Size:        337     770
 
Bme1580I G_kGCm'C

Cuts at:      0     442    1107 
   Size:        442     665
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    1107 
   Size:       1064      43
 
Bpu10I CC'TnA_GC

Cuts at:      0     337    1107 
   Size:        337     770
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     325    1107 
   Size:        325     782
 
BseYI C'CCAG_C

Cuts at:      0     492    1107 
   Size:        492     615
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    1107 
   Size:        737     370
 
BsiHKAI G_wGCw'C

Cuts at:      0     442     838    1050    1107 
   Size:        442     396     212      57

  Fragments arranged by size: 

                 442     396     212      57
 
BsmI GAATG_Cn'

Cuts at:      0     103    1107 
   Size:        103    1004
 
Bsp1286I G_dGCh'C

Cuts at:      0     442     607     838    1050    1107 
   Size:        442     165     231     212      57

  Fragments arranged by size: 

                 442     231     212     165      57
 
BspHI T'CATG_A

Cuts at:      0     554    1107 
   Size:        554     553
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     413     577    1107 
   Size:        413     164     530

  Fragments arranged by size: 

                 530     413     164
 
BsrGI T'GTAC_A

Cuts at:      0     917    1107 
   Size:        917     190
 
Bsu36I CC'TnA_GG

Cuts at:      0     219    1107 
   Size:        219     888
 
DraI TTT'AAA

Cuts at:      0      23      44    1107 
   Size:         23      21    1063

  Fragments arranged by size: 

                1063      23      21
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     891    1107 
   Size:        891     216
 
EaeI y'GGCC_r

Cuts at:      0     269     521    1107 
   Size:        269     252     586

  Fragments arranged by size: 

                 586     269     252
 
EarI CTCTTCn'nnn_

Cuts at:      0     152    1048    1107 
   Size:        152     896      59

  Fragments arranged by size: 

                 896     152      59
 
EcoICRI GAG'CTC

Cuts at:      0     836    1048    1107 
   Size:        836     212      59

  Fragments arranged by size: 

                 836     212      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1064    1072    1107 
   Size:        724     340       8      35

  Fragments arranged by size: 

                 724     340      35       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1065    1107 
   Size:       1065      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1058    1078    1107 
   Size:       1058      20      29

  Fragments arranged by size: 

                1058      29      20
 
EcoRI G'AATT_C

Cuts at:      0     481    1107 
   Size:        481     626
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1077    1107 
   Size:       1077      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     533     565    1032    1064    1107 
   Size:        533      32     467      32      43

  Fragments arranged by size: 

                 533     467      43      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     553    1107 
   Size:        553     554
 
MscI TGG'CCA

Cuts at:      0     271     523    1107 
   Size:        271     252     584

  Fragments arranged by size: 

                 584     271     252
 
MslI CAynn'nnrTG

Cuts at:      0     190     379     637     886     925     997    1107 
   Size:        190     189     258     249      39      72     110

  Fragments arranged by size: 

                 258     249     190     189     110      72      39
 
NspI r_CATG'y

Cuts at:      0      76     716     930    1107 
   Size:         76     640     214     177

  Fragments arranged by size: 

                 640     214     177      76
 
PasI CC'CwG_GG

Cuts at:      0      92    1107 
   Size:         92    1015
 
PciI A'CATG_T

Cuts at:      0      72    1107 
   Size:         72    1035
 
PpuMI rG'GwC_Cy

Cuts at:      0    1078    1107 
   Size:       1078      29
 
SacI G_AGCT'C

Cuts at:      0     838    1050    1107 
   Size:        838     212      57

  Fragments arranged by size: 

                 838     212      57
 
SfcI C'TryA_G

Cuts at:      0     143     176     785     818    1017    1107 
   Size:        143      33     609      33     199      90

  Fragments arranged by size: 

                 609     199     143      90      33      33
 
SmaI CCC'GGG

Cuts at:      0     664    1107 
   Size:        664     443
 
StyI C'CwwG_G

Cuts at:      0    1061    1107 
   Size:       1061      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     336    1011    1107 
   Size:        336     675      96

  Fragments arranged by size: 

                 675     336      96
 
TatI w'GTAC_w

Cuts at:      0     161     326     917    1107 
   Size:        161     165     591     190

  Fragments arranged by size: 

                 591     190     165     161
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     683     715    1107 
   Size:        683      32     392

  Fragments arranged by size: 

                 683     392      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     964    1107 
   Size:        964     143
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     429    1107 
   Size:        429     678
 
XmaI C'CCGG_G

Cuts at:      0     662    1107 
   Size:        662     445
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    ApaLI     ApoI     AseI     AvaI     BanI
    BanII    BbvCI Bme1580I     BpmI   Bpu10I    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrGI   Bsu36I     DraI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    Hin4I     MmeI     MscI     MslI     NspI     PasI     PciI
    PpuMI     SacI     SfcI     SmaI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     EagI     EciI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI     XmnI
     ZraI
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