Restriction map for pF1KB7420
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaI        BseAI       BseRI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MaeI        
MaeII       MfeI        MluI        MroI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AlwNI         794  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       166  AGCGCT               AGC^GCT
AscI          149  GGCGCGCC             GG^CGCGCC
AspHI         610  GAGCTC               G[AT]GC[AT]^C
BanII         610  GAGCTC               G[GA]GC[CT]^C
BbeI          629  GGCGCC               GGCGC^C
BbsI          424  GTCTTC               GAAGAC(2/6)
BbvCI         510  GCTGAGG              CCTCAGC(-5/-2)
BglII          36  AGATCT               A^GATCT
BpuAI         424  GTCTTC               GAAGAC(2/6)
BsaBI         762  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          50  TCCGGT               [AT]^CCGG[AT]
BsgI          289  CTGCAC               GTGCAG(16/14)
BsiHKAI       610  GAGCTC               G[AT]GC[AT]^C
BspMI         377  ACCTGC               ACCTGC(4/8)
BsrFI         456  GCCGGC               [GA]^CCGG[CT]
BssHII        150  GCGCGC               G^CGCGC
BstF5I        402  CATCC                GGATG(2/0)
BstXI          15  CCAGTGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        456  GCCGGC               [GA]^CCGG[CT]
DrdI          697  GACTGCCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          434  CCACGG               C^C[GA][CT]GG
EarI          730  CTCTTC               CTCTTC(1/4)
Eco47III      166  AGCGCT               AGC^GCT
EcoT14I       486  CCTTGG               C^C[AT][AT]GG
FokI          402  CATCC                GGATG(9/13)
HgaI          306  GACGC                GACGC(5/10)
HinfI         676  GACTC                G^A[ACGT]TC
KasI          629  GGCGCC               G^GCGCC
Ksp632I       730  CTCTTC               CTCTTC(1/4)
MamI          762  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I         86  CCGCTG               C[AC]G^C[GT]G
NaeI          456  GCCGGC               GCC^GGC
NarI          629  GGCGCC               GG^CGCC
NgoMIV        456  GCCGGC               G^CCGGC
PleI          676  GACTC                GAGTC(4/5)
PshAI         777  GACAGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          610  GAGCTC               GAGCT^C
SapI          729  GCTCTTC              GCTCTTC(1/4)
SfaNI         561  GATGC                GCATC(5/9)
SfoI          629  GGCGCC               GGC^GCC
StyI          486  CCTTGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          306  GACGCC               G[GA]^CG[CT]C
              629  GGCGCC              
AlwI          387  GATCC                GGATC(4/5)
              467  GGATC               
AvaII         572  GGACC                G^G[AT]CC
              783  GGTCC               
BalI          431  TGGCCA               TGG^CCA
              489  TGGCCA              
BsaHI         306  GACGCC               G[GA]^CG[CT]C
              629  GGCGCC              
BstYI          36  AGATCT               [GA]^GATC[CT]
              386  AGATCC              
Cfr13I        572  GGACC                G^G[ACGT]CC
              783  GGTCC               
DraII         571  GGGACCC              [GA]G^G[ACGT]CC[CT]
              782  AGGTCCT             
EaeI          431  TGGCCA               [CT]^GGCC[GA]
              489  TGGCCA              
EcoO109I      571  GGGACCC              [GA]G^G[ACGT]CC[CT]
              782  AGGTCCT             
FauI          451  CCCGC                CCCGC(4/6)
              283  GCGGG               
HaeII         166  AGCGCT               [GA]GCGC^[CT]
              629  GGCGCC              
Hin1I         306  GACGCC               G[GA]^CG[CT]C
              629  GGCGCC              
HincII        112  GTCAAC               GT[CT]^[GA]AC
              397  GTTGAC              
HindII        112  GTCAAC               GT[CT]^[GA]AC
              397  GTTGAC              
MaeIII        157  GTCAC                ^GT[ACGT]AC
              304  GTGAC               
MboII         425  TCTTC                GAAGA(8/7)
              731  TCTTC               
MflI           36  AGATCT               [GA]^GATC[CT]
              386  AGATCC              
MluNI         431  TGGCCA               TGG^CCA
              489  TGGCCA              
MscI          431  TGGCCA               TGG^CCA
              489  TGGCCA              
MslI          435  CACGGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              555  CACCATGATG          
NspI          320  GCATGT               [GA]CATG^[CT]
              440  ACATGC              
PpuMI         571  GGGACCC              [GA]G^G[AT]CC[CT]
              782  AGGTCCT             
Sau96I        572  GGACC                G^G[ACGT]CC
              783  GGTCC               
SmlI          634  CTCAAG               C^T[CT][GA]AG
              664  CTTGAG              
Tsp45I        157  GTCAC                ^GT[GC]AC
              304  GTGAC               
XhoII          36  AGATCT               [GA]^GATC[CT]
              386  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AccII       AciI        AluI        BanI        BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        DdeI        
DpnII       EcoRII      Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MboI        MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      ScrFI       TseI        TspRI       
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