Restriction map for pF1KB7417
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Cac8I       CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AocI          447  CCTGAGG              CC^T[ACGT]AGG
AvaI         1020  CCCGGG               C^[CT]CG[GA]G
BanI          517  GGCACC               G^G[CT][GA]CC
BbsI          679  GTCTTC               GAAGAC(2/6)
BciVI          26  GGATAC               GTATCC(6/5)
BmyI          418  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         679  GTCTTC               GAAGAC(2/6)
BsaI          743  GAGACC               GGTCTC(1/5)
BsmFI         407  GGGAC                GGGAC(10/14)
BsoBI        1020  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      418  GGGCAC               G[AGT]GC[ACT]^C
BspMI         481  GCAGGT               ACCTGC(4/8)
BsrDI        1003  CATTGC               GCAATG(2/0)
BstAPI       1007  GCAACCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1053  CCACGTAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         281  AGATCC               [GA]^GATC[CT]
Bsu36I        447  CCTGAGG              CC^T[ACGT]AGG
CfoI         1080  GCGC                 GCG^C
Eco81I        447  CCTGAGG              CC^T[ACGT]AGG
EcoNI         393  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII        1079  AGCGCC               [GA]GCGC^[CT]
HhaI         1080  GCGC                 GCG^C
HinP1I       1080  GCGC                 G^CGC
HincII         75  GTTGAC               GT[CT]^[GA]AC
HindII         75  GTTGAC               GT[CT]^[GA]AC
MflI          281  AGATCC               [GA]^GATC[CT]
MseI           88  TTAA                 T^TAA
MspA1I        382  CAGCTG               C[AC]G^C[GT]G
NdeI          778  CATATG               CA^TATG
PflMI         726  CCAGTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1042  GACTC                GAGTC(4/5)
PstI          369  CTGCAG               CTGCA^G
PvuII         382  CAGCTG               CAG^CTG
SexAI         524  ACCTGGT              A^CC[AT]GGT
SfcI          369  CTGCAG               C^T[GA][CT]AG
SmaI         1020  CCCGGG               CCC^GGG
SmlI          356  CTCAAG               C^T[CT][GA]AG
SnaBI         363  TACGTA               TAC^GTA
TfiI         1033  GATTC                G^A[AT]TC
Tru9I          88  TTAA                 T^TAA
Van91I        726  CCAGTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          467  CCACAGCACTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         281  AGATCC               [GA]^GATC[CT]
XmaCI        1020  CCCGGG               C^CCGGG
XmaI         1020  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Asp700I       174  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              821  GAAATATTTC          
AvaII         408  GGACC                G^G[AT]CC
              866  GGTCC               
BbvI         1077  GCAGC                GCAGC(8/12)
              573  GCTGC               
BfaI          271  CTAG                 C^TAG
              812  CTAG                
BsaAI         363  TACGTA               [CT]AC^GT[GA]
             1054  CACGTA              
BsgI          981  GTGCAG               GTGCAG(16/14)
             1075  GTGCAG              
EcoT14I       168  CCAAGG               C^C[AT][AT]GG
              624  CCAAGG              
Fnu4HI        573  GCTGC                GC^[ACGT]GC
             1077  GCAGC               
HapII         925  CCGG                 C^CGG
             1021  CCGG                
HinfI        1033  GATTC                G^A[ACGT]TC
             1042  GACTC               
HpaII         925  CCGG                 C^CGG
             1021  CCGG                
ItaI          573  GCTGC                GC^[ACGT]GC
             1077  GCAGC               
MaeI          271  CTAG                 C^TAG
              812  CTAG                
MaeII         364  ACGT                 A^CGT
             1055  ACGT                
MaeIII        499  GTGAC                ^GT[ACGT]AC
              966  GTCAC               
MspI          925  CCGG                 C^CGG
             1021  CCGG                
SspI          188  AATATT               AAT^ATT
              823  AATATT              
StuI          769  AGGCCT               AGG^CCT
              791  AGGCCT              
StyI          168  CCAAGG               C^C[AT][AT]GG
              624  CCAAGG              
TaqI           57  TCGA                 T^CGA
              948  TCGA                
TseI          573  GCTGC                G^C[AT]GC
             1077  GCAGC               
Tsp45I        499  GTGAC                ^GT[GC]AC
              966  GTCAC               
Tsp509I        62  AATT                 ^AATT
               90  AATT                
TthHB8I        57  TCGA                 T^CGA
              948  TCGA                
XmnI          174  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              821  GAAATATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AfaI        AluI        AlwI        BcnI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       EcoRII      FokI        HaeIII      HphI        
MboI        MboII       MnlI        MslI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       TspRI       
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