Restriction map for pF1KB7416
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbrPI       BbsI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaWI       BseAI       
BsiWI       BspDI       BspEI       BspMI       BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        Eam1105I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MaeI        
MamI        MfeI        MroI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         166  GACGTC               GACGT^C
AocI          342  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       705  AGCGCT               AGC^GCT
BalI          380  TGGCCA               TGG^CCA
BbeI          226  GGCGCC               GGCGC^C
BcgI          161  GCAGCGACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1246  GCCCTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1302  TACGTG               [CT]AC^GT[GA]
BsiEI          27  CGGCCG               CG[GA][CT]^CG
BsmI          852  GAATGC               GAATGC(1/-1)
Bsp1407I     1238  TGTACA               T^GTACA
BspLU11I     1235  ACATGT               A^CATGT
BsrBI          67  GAGCGG               CCGCTC(-3/-3)
BsrDI         543  CATTGC               GCAATG(2/0)
BsrGI        1238  TGTACA               T^GTACA
BssSI         790  CTCGTG               CACGAG(-5/-1)
BstEII        132  GGTCACC              G^GT[ACGT]ACC
BstPI         132  GGTCACC              G^GT[ACGT]ACC
Bsu36I        342  CCTGAGG              CC^T[ACGT]AGG
DraIII        148  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          532  GACAGGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           27  CGGCCG               C^GGCCG
EarI         1281  GAAGAG               CTCTTC(1/4)
EclXI          27  CGGCCG               C^GGCCG
Eco47III      705  AGCGCT               AGC^GCT
Eco52I         27  CGGCCG               C^GGCCG
Eco81I        342  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       132  GGTCACC              G^GT[ACGT]ACC
HincII       1099  GTCAAC               GT[CT]^[GA]AC
HindII       1099  GTCAAC               GT[CT]^[GA]AC
HinfI        1207  GACTC                G^A[ACGT]TC
KasI          226  GGCGCC               G^GCGCC
Ksp632I      1281  GAAGAG               CTCTTC(1/4)
MluI         1287  ACGCGT               A^CGCGT
MluNI         380  TGGCCA               TGG^CCA
MscI          380  TGGCCA               TGG^CCA
NarI          226  GGCGCC               GG^CGCC
NcoI         1118  CCATGG               C^CATGG
PaeR7I       1320  CTCGAG               C^TCGAG
PflMI         945  CCACTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1207  GACTC                GAGTC(4/5)
PstI          353  CTGCAG               CTGCA^G
SacI          916  GAGCTC               GAGCT^C
ScaI          692  AGTACT               AGT^ACT
SexAI         473  ACCTGGT              A^CC[AT]GGT
SfoI          226  GGCGCC               GGC^GCC
SphI           83  GCATGC               GCATG^C
SspBI        1238  TGTACA               T^GTACA
Van91I        945  CCACTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1320  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII       1235  ACATGT               A^C[GA][CT]GT
             1287  ACGCGT              
ApaI          593  GGGCCC               GGGCC^C
              941  GGGCCC              
AspHI         649  GTGCTC               G[AT]GC[AT]^C
              916  GAGCTC              
BamHI         628  GGATCC               G^GATCC
             1265  GGATCC              
BanI          226  GGCGCC               G^G[CT][GA]CC
              800  GGTGCC              
BbvCI         987  CCTCAGC              CCTCAGC(-5/-2)
              501  GCTGAGG             
BsiHKAI       649  GTGCTC               G[AT]GC[AT]^C
              916  GAGCTC              
EcoNI        1211  CCTACCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1251  CCTGGCAGAGG         
EcoT14I      1118  CCATGG               C^C[AT][AT]GG
             1216  CCAAGG              
MaeII         167  ACGT                 A^CGT
             1303  ACGT                
MaeIII        133  GTCAC                ^GT[ACGT]AC
              154  GTGAC               
NaeI            7  GCCGGC               GCC^GGC
              348  GCCGGC              
NgoMIV          7  GCCGGC               G^CCGGC
              348  GCCGGC              
PvuII         499  CAGCTG               CAG^CTG
             1177  CAGCTG              
SfcI          306  CTACAG               C^T[GA][CT]AG
              353  CTGCAG              
SmaI           35  CCCGGG               CCC^GGG
              589  CCCGGG              
SmlI          742  CTTGAG               C^T[CT][GA]AG
             1320  CTCGAG              
StyI         1118  CCATGG               C^C[AT][AT]GG
             1216  CCAAGG              
Tsp45I        133  GTCAC                ^GT[GC]AC
              154  GTGAC               
XmaCI          35  CCCGGG               C^CCGGG
              589  CCCGGG              
XmaI           35  CCCGGG               C^CCGGG
              589  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PpuMI       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TspRI       TthHB8I     XhoII       
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